Antibody labelling of resilin in energy stores for jumping in plant sucking insects.

The rubbery protein resilin appears to form an integral part of the energy storage structures that enable many insects to jump by using a catapult mechanism. In plant sucking bugs that jump (Hemiptera, Auchenorrhyncha), the energy generated by the slow contractions of huge thoracic jumping muscles i...

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Autores principales:	Malcolm Burrows, Jolanta A Borycz, Stephen R Shaw, Christopher M Elvin, Ian A Meinertzhagen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d425c19c0a549658650bbd71066ef8c
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spelling	oai:doaj.org-article:9d425c19c0a549658650bbd71066ef8c2021-11-18T07:32:50ZAntibody labelling of resilin in energy stores for jumping in plant sucking insects.1932-620310.1371/journal.pone.0028456https://doaj.org/article/9d425c19c0a549658650bbd71066ef8c2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22163306/?tool=EBIhttps://doaj.org/toc/1932-6203The rubbery protein resilin appears to form an integral part of the energy storage structures that enable many insects to jump by using a catapult mechanism. In plant sucking bugs that jump (Hemiptera, Auchenorrhyncha), the energy generated by the slow contractions of huge thoracic jumping muscles is stored by bending composite bow-shaped parts of the internal thoracic skeleton. Sudden recoil of these bows powers the rapid and simultaneous movements of both hind legs that in turn propel a jump. Until now, identification of resilin at these storage sites has depended exclusively upon characteristics that may not be specific: its fluorescence when illuminated with specific wavelengths of ultraviolet (UV) light and extinction of that fluorescence at low pH. To consolidate identification we have labelled the cuticular structures involved with an antibody raised against a product of the Drosophila CG15920 gene. This encodes pro-resilin, the first exon of which was expressed in E. coli and used to raise the antibody. We show that in frozen sections from two species, the antibody labels precisely those parts of the metathoracic energy stores that fluoresce under UV illumination. The presence of resilin in these insects is thus now further supported by a molecular criterion that is immunohistochemically specific.Malcolm BurrowsJolanta A BoryczStephen R ShawChristopher M ElvinIan A MeinertzhagenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28456 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Malcolm Burrows Jolanta A Borycz Stephen R Shaw Christopher M Elvin Ian A Meinertzhagen Antibody labelling of resilin in energy stores for jumping in plant sucking insects.
description	The rubbery protein resilin appears to form an integral part of the energy storage structures that enable many insects to jump by using a catapult mechanism. In plant sucking bugs that jump (Hemiptera, Auchenorrhyncha), the energy generated by the slow contractions of huge thoracic jumping muscles is stored by bending composite bow-shaped parts of the internal thoracic skeleton. Sudden recoil of these bows powers the rapid and simultaneous movements of both hind legs that in turn propel a jump. Until now, identification of resilin at these storage sites has depended exclusively upon characteristics that may not be specific: its fluorescence when illuminated with specific wavelengths of ultraviolet (UV) light and extinction of that fluorescence at low pH. To consolidate identification we have labelled the cuticular structures involved with an antibody raised against a product of the Drosophila CG15920 gene. This encodes pro-resilin, the first exon of which was expressed in E. coli and used to raise the antibody. We show that in frozen sections from two species, the antibody labels precisely those parts of the metathoracic energy stores that fluoresce under UV illumination. The presence of resilin in these insects is thus now further supported by a molecular criterion that is immunohistochemically specific.
format	article
author	Malcolm Burrows Jolanta A Borycz Stephen R Shaw Christopher M Elvin Ian A Meinertzhagen
author_facet	Malcolm Burrows Jolanta A Borycz Stephen R Shaw Christopher M Elvin Ian A Meinertzhagen
author_sort	Malcolm Burrows
title	Antibody labelling of resilin in energy stores for jumping in plant sucking insects.
title_short	Antibody labelling of resilin in energy stores for jumping in plant sucking insects.
title_full	Antibody labelling of resilin in energy stores for jumping in plant sucking insects.
title_fullStr	Antibody labelling of resilin in energy stores for jumping in plant sucking insects.
title_full_unstemmed	Antibody labelling of resilin in energy stores for jumping in plant sucking insects.
title_sort	antibody labelling of resilin in energy stores for jumping in plant sucking insects.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/9d425c19c0a549658650bbd71066ef8c
work_keys_str_mv	AT malcolmburrows antibodylabellingofresilininenergystoresforjumpinginplantsuckinginsects AT jolantaaborycz antibodylabellingofresilininenergystoresforjumpinginplantsuckinginsects AT stephenrshaw antibodylabellingofresilininenergystoresforjumpinginplantsuckinginsects AT christophermelvin antibodylabellingofresilininenergystoresforjumpinginplantsuckinginsects AT ianameinertzhagen antibodylabellingofresilininenergystoresforjumpinginplantsuckinginsects
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Antibody labelling of resilin in energy stores for jumping in plant sucking insects.

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