Effects of Lespedeza Cuneata aqueous extract on testosterone-induced prostatic hyperplasia

Context: Lespedeza cuneata G. Don (Fabaceae), has been used as a traditional treatment of various diseases. There is a report L. cuneata effects on hormone replacement therapy for endocrine-related disease. However, studies related to benign prostatic hyperplasia (BPH) have not been investigated. Ob...

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Autores principales:	Bong Kyun Park, Chang Won Kim, Jeong Eun Kwon, Manorma Negi, Yong Tae Koo, Sang Hun Lee, Dong Hyun Baek, Yoo Hun Noh, Se Chan Kang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	pcna inflammation androgen receptor Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/9d43cfa78b5d4e14848cc29eb314d7a7
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spelling	oai:doaj.org-article:9d43cfa78b5d4e14848cc29eb314d7a72021-11-17T14:21:55ZEffects of Lespedeza Cuneata aqueous extract on testosterone-induced prostatic hyperplasia1388-02091744-511610.1080/13880209.2018.1564929https://doaj.org/article/9d43cfa78b5d4e14848cc29eb314d7a72019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2018.1564929https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Lespedeza cuneata G. Don (Fabaceae), has been used as a traditional treatment of various diseases. There is a report L. cuneata effects on hormone replacement therapy for endocrine-related disease. However, studies related to benign prostatic hyperplasia (BPH) have not been investigated. Objective: The effects of L. cuneata aqueous extract (LCW) on testosterone-induced prostatic hyperplasia (TPH) were examined. Materials and methods: Male Wistar rats (10 weeks, 330–350 g) were randomly divided to 6 groups (n = 6): Control group; TPH group (3 mg/kg, s.c, daily); TPH + LCW (25, 50, 100 mg/kg); TPH + Finasteride 10 mg/kg for 6 weeks. At the end of treatment, histological change of prostate, serum dihydrotestosterone (DHT) level, mRNA expression of 5α-reductase, inflammatory factors, proliferating cell nuclear antigen (PCNA) and fibroblast growth factor-2 (FGF-2) in prostate were examined. Then, LCW was treated with BPH-1, a human BPH cell line, at 25, 50, 100 μg/mL for 24 h and examine mRNA level of androgen receptor (AR) and prostate-specific antigen (PSA). In addition, the content of vicenin-2 was analyzed. Results: LCW treatment of TPH inhibited serum DHT levels by 54.5, 51.2 and 54.1% and mRNA expression of 5α-reductase were inhibited 54.3, 61.3 and 73.6%, respectively. In addition, mRNA expression of inflammatory factors, PCNA and FGF-2 were decreased in the prostate of rats. Also, LCW attenuated mRNA level of AR and PSA in BPH-1 cell. The content of vicenin-2 in the LCW was analyzed to 0.89 mg/g. Discussion and conclusions: Based on the results, LCW is a potential pharmacological candidate for the treatment of prostatic hyperplasia.Bong Kyun ParkChang Won KimJeong Eun KwonManorma NegiYong Tae KooSang Hun LeeDong Hyun BaekYoo Hun NohSe Chan KangTaylor & Francis Grouparticlepcnainflammationandrogen receptorTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 89-97 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	pcna inflammation androgen receptor Therapeutics. Pharmacology RM1-950
spellingShingle	pcna inflammation androgen receptor Therapeutics. Pharmacology RM1-950 Bong Kyun Park Chang Won Kim Jeong Eun Kwon Manorma Negi Yong Tae Koo Sang Hun Lee Dong Hyun Baek Yoo Hun Noh Se Chan Kang Effects of Lespedeza Cuneata aqueous extract on testosterone-induced prostatic hyperplasia
description	Context: Lespedeza cuneata G. Don (Fabaceae), has been used as a traditional treatment of various diseases. There is a report L. cuneata effects on hormone replacement therapy for endocrine-related disease. However, studies related to benign prostatic hyperplasia (BPH) have not been investigated. Objective: The effects of L. cuneata aqueous extract (LCW) on testosterone-induced prostatic hyperplasia (TPH) were examined. Materials and methods: Male Wistar rats (10 weeks, 330–350 g) were randomly divided to 6 groups (n = 6): Control group; TPH group (3 mg/kg, s.c, daily); TPH + LCW (25, 50, 100 mg/kg); TPH + Finasteride 10 mg/kg for 6 weeks. At the end of treatment, histological change of prostate, serum dihydrotestosterone (DHT) level, mRNA expression of 5α-reductase, inflammatory factors, proliferating cell nuclear antigen (PCNA) and fibroblast growth factor-2 (FGF-2) in prostate were examined. Then, LCW was treated with BPH-1, a human BPH cell line, at 25, 50, 100 μg/mL for 24 h and examine mRNA level of androgen receptor (AR) and prostate-specific antigen (PSA). In addition, the content of vicenin-2 was analyzed. Results: LCW treatment of TPH inhibited serum DHT levels by 54.5, 51.2 and 54.1% and mRNA expression of 5α-reductase were inhibited 54.3, 61.3 and 73.6%, respectively. In addition, mRNA expression of inflammatory factors, PCNA and FGF-2 were decreased in the prostate of rats. Also, LCW attenuated mRNA level of AR and PSA in BPH-1 cell. The content of vicenin-2 in the LCW was analyzed to 0.89 mg/g. Discussion and conclusions: Based on the results, LCW is a potential pharmacological candidate for the treatment of prostatic hyperplasia.
format	article
author	Bong Kyun Park Chang Won Kim Jeong Eun Kwon Manorma Negi Yong Tae Koo Sang Hun Lee Dong Hyun Baek Yoo Hun Noh Se Chan Kang
author_facet	Bong Kyun Park Chang Won Kim Jeong Eun Kwon Manorma Negi Yong Tae Koo Sang Hun Lee Dong Hyun Baek Yoo Hun Noh Se Chan Kang
author_sort	Bong Kyun Park
title	Effects of Lespedeza Cuneata aqueous extract on testosterone-induced prostatic hyperplasia
title_short	Effects of Lespedeza Cuneata aqueous extract on testosterone-induced prostatic hyperplasia
title_full	Effects of Lespedeza Cuneata aqueous extract on testosterone-induced prostatic hyperplasia
title_fullStr	Effects of Lespedeza Cuneata aqueous extract on testosterone-induced prostatic hyperplasia
title_full_unstemmed	Effects of Lespedeza Cuneata aqueous extract on testosterone-induced prostatic hyperplasia
title_sort	effects of lespedeza cuneata aqueous extract on testosterone-induced prostatic hyperplasia
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/9d43cfa78b5d4e14848cc29eb314d7a7
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Effects of Lespedeza Cuneata aqueous extract on testosterone-induced prostatic hyperplasia

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