Variability in bacterial flagella re-growth patterns after breakage

Abstract Many bacteria swim through liquids or crawl on surfaces by rotating long appendages called flagella. Flagellar filaments are assembled from thousands of subunits that are exported through a narrow secretion channel and polymerize beneath a capping scaffold at the tip of the growing filament...

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Autores principales:	Guillaume Paradis, Fabienne F. V. Chevance, Willisa Liou, Thibaud T. Renault, Kelly T. Hughes, Simon Rainville, Marc Erhardt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d48816ce76f42f4a0ce35b547ad1dd8
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spelling	oai:doaj.org-article:9d48816ce76f42f4a0ce35b547ad1dd82021-12-02T12:30:43ZVariability in bacterial flagella re-growth patterns after breakage10.1038/s41598-017-01302-52045-2322https://doaj.org/article/9d48816ce76f42f4a0ce35b547ad1dd82017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01302-5https://doaj.org/toc/2045-2322Abstract Many bacteria swim through liquids or crawl on surfaces by rotating long appendages called flagella. Flagellar filaments are assembled from thousands of subunits that are exported through a narrow secretion channel and polymerize beneath a capping scaffold at the tip of the growing filament. The assembly of a flagellum uses a significant proportion of the biosynthetic capacities of the cell with each filament constituting ~1% of the total cell protein. Here, we addressed a significant question whether a flagellar filament can form a new cap and resume growth after breakage. Re-growth of broken filaments was visualized using sequential 3-color fluorescent labeling of filaments after mechanical shearing. Differential electron microscopy revealed the formation of new cap structures on broken filaments that re-grew. Flagellar filaments are therefore able to re-grow if broken by mechanical shearing forces, which are expected to occur frequently in nature. In contrast, no re-growth was observed on filaments that had been broken using ultrashort laser pulses, a technique allowing for very local damage to individual filaments. We thus conclude that assembly of a new cap at the tip of a broken filament depends on how the filament was broken.Guillaume ParadisFabienne F. V. ChevanceWillisa LiouThibaud T. RenaultKelly T. HughesSimon RainvilleMarc ErhardtNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guillaume Paradis Fabienne F. V. Chevance Willisa Liou Thibaud T. Renault Kelly T. Hughes Simon Rainville Marc Erhardt Variability in bacterial flagella re-growth patterns after breakage
description	Abstract Many bacteria swim through liquids or crawl on surfaces by rotating long appendages called flagella. Flagellar filaments are assembled from thousands of subunits that are exported through a narrow secretion channel and polymerize beneath a capping scaffold at the tip of the growing filament. The assembly of a flagellum uses a significant proportion of the biosynthetic capacities of the cell with each filament constituting ~1% of the total cell protein. Here, we addressed a significant question whether a flagellar filament can form a new cap and resume growth after breakage. Re-growth of broken filaments was visualized using sequential 3-color fluorescent labeling of filaments after mechanical shearing. Differential electron microscopy revealed the formation of new cap structures on broken filaments that re-grew. Flagellar filaments are therefore able to re-grow if broken by mechanical shearing forces, which are expected to occur frequently in nature. In contrast, no re-growth was observed on filaments that had been broken using ultrashort laser pulses, a technique allowing for very local damage to individual filaments. We thus conclude that assembly of a new cap at the tip of a broken filament depends on how the filament was broken.
format	article
author	Guillaume Paradis Fabienne F. V. Chevance Willisa Liou Thibaud T. Renault Kelly T. Hughes Simon Rainville Marc Erhardt
author_facet	Guillaume Paradis Fabienne F. V. Chevance Willisa Liou Thibaud T. Renault Kelly T. Hughes Simon Rainville Marc Erhardt
author_sort	Guillaume Paradis
title	Variability in bacterial flagella re-growth patterns after breakage
title_short	Variability in bacterial flagella re-growth patterns after breakage
title_full	Variability in bacterial flagella re-growth patterns after breakage
title_fullStr	Variability in bacterial flagella re-growth patterns after breakage
title_full_unstemmed	Variability in bacterial flagella re-growth patterns after breakage
title_sort	variability in bacterial flagella re-growth patterns after breakage
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9d48816ce76f42f4a0ce35b547ad1dd8
work_keys_str_mv	AT guillaumeparadis variabilityinbacterialflagellaregrowthpatternsafterbreakage AT fabiennefvchevance variabilityinbacterialflagellaregrowthpatternsafterbreakage AT willisaliou variabilityinbacterialflagellaregrowthpatternsafterbreakage AT thibaudtrenault variabilityinbacterialflagellaregrowthpatternsafterbreakage AT kellythughes variabilityinbacterialflagellaregrowthpatternsafterbreakage AT simonrainville variabilityinbacterialflagellaregrowthpatternsafterbreakage AT marcerhardt variabilityinbacterialflagellaregrowthpatternsafterbreakage
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Variability in bacterial flagella re-growth patterns after breakage

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