The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population

Abstract The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and genetic differentiation in a highly clonal sympatric Sinorhizobium melilo...

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Autores principales:	Nicolás Toro, Pablo J. Villadas, María Dolores Molina-Sánchez, Pilar Navarro-Gómez, José M. Vinardell, Lidia Cuesta-Berrio, Miguel A. Rodríguez-Carvajal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d49c55c01cd487faa81b8622b75699d
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spelling	oai:doaj.org-article:9d49c55c01cd487faa81b8622b75699d2021-12-02T12:32:40ZThe underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population10.1038/s41598-017-00730-72045-2322https://doaj.org/article/9d49c55c01cd487faa81b8622b75699d2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00730-7https://doaj.org/toc/2045-2322Abstract The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and genetic differentiation in a highly clonal sympatric Sinorhizobium meliloti population. Whole-genome sequencing revealed that a large DNA region of the symbiotic plasmid pSymB was replaced in some isolates with a similar synteny block carrying densely clustered SNPs and displaying gene acquisition and loss. Two different versions of this genomic island of differentiation (GID) generated by multiple genetic exchanges over time appear to have arisen recently, through recombination in a particular clade within this population. In addition, these isolates display resistance to phages from the same geographic region, probably due to the modification of surface components by the acquired genes. Our results suggest that an underlying process of early ecological and genetic differentiation in S. meliloti is primarily triggered by acquisition of genes that confer resistance to soil phages within particular large genomic DNA regions prone to recombination.Nicolás ToroPablo J. VilladasMaría Dolores Molina-SánchezPilar Navarro-GómezJosé M. VinardellLidia Cuesta-BerrioMiguel A. Rodríguez-CarvajalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicolás Toro Pablo J. Villadas María Dolores Molina-Sánchez Pilar Navarro-Gómez José M. Vinardell Lidia Cuesta-Berrio Miguel A. Rodríguez-Carvajal The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
description	Abstract The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and genetic differentiation in a highly clonal sympatric Sinorhizobium meliloti population. Whole-genome sequencing revealed that a large DNA region of the symbiotic plasmid pSymB was replaced in some isolates with a similar synteny block carrying densely clustered SNPs and displaying gene acquisition and loss. Two different versions of this genomic island of differentiation (GID) generated by multiple genetic exchanges over time appear to have arisen recently, through recombination in a particular clade within this population. In addition, these isolates display resistance to phages from the same geographic region, probably due to the modification of surface components by the acquired genes. Our results suggest that an underlying process of early ecological and genetic differentiation in S. meliloti is primarily triggered by acquisition of genes that confer resistance to soil phages within particular large genomic DNA regions prone to recombination.
format	article
author	Nicolás Toro Pablo J. Villadas María Dolores Molina-Sánchez Pilar Navarro-Gómez José M. Vinardell Lidia Cuesta-Berrio Miguel A. Rodríguez-Carvajal
author_facet	Nicolás Toro Pablo J. Villadas María Dolores Molina-Sánchez Pilar Navarro-Gómez José M. Vinardell Lidia Cuesta-Berrio Miguel A. Rodríguez-Carvajal
author_sort	Nicolás Toro
title	The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
title_short	The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
title_full	The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
title_fullStr	The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
title_full_unstemmed	The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
title_sort	underlying process of early ecological and genetic differentiation in a facultative mutualistic sinorhizobium meliloti population
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9d49c55c01cd487faa81b8622b75699d
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The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population

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