The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population

Abstract The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and genetic differentiation in a highly clonal sympatric Sinorhizobium melilo...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Nicolás Toro, Pablo J. Villadas, María Dolores Molina-Sánchez, Pilar Navarro-Gómez, José M. Vinardell, Lidia Cuesta-Berrio, Miguel A. Rodríguez-Carvajal
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
R
Q
Acceso en línea:https://doaj.org/article/9d49c55c01cd487faa81b8622b75699d
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:9d49c55c01cd487faa81b8622b75699d
record_format dspace
spelling oai:doaj.org-article:9d49c55c01cd487faa81b8622b75699d2021-12-02T12:32:40ZThe underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population10.1038/s41598-017-00730-72045-2322https://doaj.org/article/9d49c55c01cd487faa81b8622b75699d2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00730-7https://doaj.org/toc/2045-2322Abstract The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and genetic differentiation in a highly clonal sympatric Sinorhizobium meliloti population. Whole-genome sequencing revealed that a large DNA region of the symbiotic plasmid pSymB was replaced in some isolates with a similar synteny block carrying densely clustered SNPs and displaying gene acquisition and loss. Two different versions of this genomic island of differentiation (GID) generated by multiple genetic exchanges over time appear to have arisen recently, through recombination in a particular clade within this population. In addition, these isolates display resistance to phages from the same geographic region, probably due to the modification of surface components by the acquired genes. Our results suggest that an underlying process of early ecological and genetic differentiation in S. meliloti is primarily triggered by acquisition of genes that confer resistance to soil phages within particular large genomic DNA regions prone to recombination.Nicolás ToroPablo J. VilladasMaría Dolores Molina-SánchezPilar Navarro-GómezJosé M. VinardellLidia Cuesta-BerrioMiguel A. Rodríguez-CarvajalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Nicolás Toro
Pablo J. Villadas
María Dolores Molina-Sánchez
Pilar Navarro-Gómez
José M. Vinardell
Lidia Cuesta-Berrio
Miguel A. Rodríguez-Carvajal
The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
description Abstract The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and genetic differentiation in a highly clonal sympatric Sinorhizobium meliloti population. Whole-genome sequencing revealed that a large DNA region of the symbiotic plasmid pSymB was replaced in some isolates with a similar synteny block carrying densely clustered SNPs and displaying gene acquisition and loss. Two different versions of this genomic island of differentiation (GID) generated by multiple genetic exchanges over time appear to have arisen recently, through recombination in a particular clade within this population. In addition, these isolates display resistance to phages from the same geographic region, probably due to the modification of surface components by the acquired genes. Our results suggest that an underlying process of early ecological and genetic differentiation in S. meliloti is primarily triggered by acquisition of genes that confer resistance to soil phages within particular large genomic DNA regions prone to recombination.
format article
author Nicolás Toro
Pablo J. Villadas
María Dolores Molina-Sánchez
Pilar Navarro-Gómez
José M. Vinardell
Lidia Cuesta-Berrio
Miguel A. Rodríguez-Carvajal
author_facet Nicolás Toro
Pablo J. Villadas
María Dolores Molina-Sánchez
Pilar Navarro-Gómez
José M. Vinardell
Lidia Cuesta-Berrio
Miguel A. Rodríguez-Carvajal
author_sort Nicolás Toro
title The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
title_short The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
title_full The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
title_fullStr The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
title_full_unstemmed The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
title_sort underlying process of early ecological and genetic differentiation in a facultative mutualistic sinorhizobium meliloti population
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/9d49c55c01cd487faa81b8622b75699d
work_keys_str_mv AT nicolastoro theunderlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation
AT pablojvilladas theunderlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation
AT mariadoloresmolinasanchez theunderlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation
AT pilarnavarrogomez theunderlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation
AT josemvinardell theunderlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation
AT lidiacuestaberrio theunderlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation
AT miguelarodriguezcarvajal theunderlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation
AT nicolastoro underlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation
AT pablojvilladas underlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation
AT mariadoloresmolinasanchez underlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation
AT pilarnavarrogomez underlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation
AT josemvinardell underlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation
AT lidiacuestaberrio underlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation
AT miguelarodriguezcarvajal underlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation
_version_ 1718393995113005056