The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
Abstract The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and genetic differentiation in a highly clonal sympatric Sinorhizobium melilo...
Guardado en:
Autores principales: | , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2017
|
Materias: | |
Acceso en línea: | https://doaj.org/article/9d49c55c01cd487faa81b8622b75699d |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:9d49c55c01cd487faa81b8622b75699d |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:9d49c55c01cd487faa81b8622b75699d2021-12-02T12:32:40ZThe underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population10.1038/s41598-017-00730-72045-2322https://doaj.org/article/9d49c55c01cd487faa81b8622b75699d2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00730-7https://doaj.org/toc/2045-2322Abstract The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and genetic differentiation in a highly clonal sympatric Sinorhizobium meliloti population. Whole-genome sequencing revealed that a large DNA region of the symbiotic plasmid pSymB was replaced in some isolates with a similar synteny block carrying densely clustered SNPs and displaying gene acquisition and loss. Two different versions of this genomic island of differentiation (GID) generated by multiple genetic exchanges over time appear to have arisen recently, through recombination in a particular clade within this population. In addition, these isolates display resistance to phages from the same geographic region, probably due to the modification of surface components by the acquired genes. Our results suggest that an underlying process of early ecological and genetic differentiation in S. meliloti is primarily triggered by acquisition of genes that confer resistance to soil phages within particular large genomic DNA regions prone to recombination.Nicolás ToroPablo J. VilladasMaría Dolores Molina-SánchezPilar Navarro-GómezJosé M. VinardellLidia Cuesta-BerrioMiguel A. Rodríguez-CarvajalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Nicolás Toro Pablo J. Villadas María Dolores Molina-Sánchez Pilar Navarro-Gómez José M. Vinardell Lidia Cuesta-Berrio Miguel A. Rodríguez-Carvajal The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population |
description |
Abstract The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and genetic differentiation in a highly clonal sympatric Sinorhizobium meliloti population. Whole-genome sequencing revealed that a large DNA region of the symbiotic plasmid pSymB was replaced in some isolates with a similar synteny block carrying densely clustered SNPs and displaying gene acquisition and loss. Two different versions of this genomic island of differentiation (GID) generated by multiple genetic exchanges over time appear to have arisen recently, through recombination in a particular clade within this population. In addition, these isolates display resistance to phages from the same geographic region, probably due to the modification of surface components by the acquired genes. Our results suggest that an underlying process of early ecological and genetic differentiation in S. meliloti is primarily triggered by acquisition of genes that confer resistance to soil phages within particular large genomic DNA regions prone to recombination. |
format |
article |
author |
Nicolás Toro Pablo J. Villadas María Dolores Molina-Sánchez Pilar Navarro-Gómez José M. Vinardell Lidia Cuesta-Berrio Miguel A. Rodríguez-Carvajal |
author_facet |
Nicolás Toro Pablo J. Villadas María Dolores Molina-Sánchez Pilar Navarro-Gómez José M. Vinardell Lidia Cuesta-Berrio Miguel A. Rodríguez-Carvajal |
author_sort |
Nicolás Toro |
title |
The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population |
title_short |
The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population |
title_full |
The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population |
title_fullStr |
The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population |
title_full_unstemmed |
The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population |
title_sort |
underlying process of early ecological and genetic differentiation in a facultative mutualistic sinorhizobium meliloti population |
publisher |
Nature Portfolio |
publishDate |
2017 |
url |
https://doaj.org/article/9d49c55c01cd487faa81b8622b75699d |
work_keys_str_mv |
AT nicolastoro theunderlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation AT pablojvilladas theunderlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation AT mariadoloresmolinasanchez theunderlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation AT pilarnavarrogomez theunderlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation AT josemvinardell theunderlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation AT lidiacuestaberrio theunderlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation AT miguelarodriguezcarvajal theunderlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation AT nicolastoro underlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation AT pablojvilladas underlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation AT mariadoloresmolinasanchez underlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation AT pilarnavarrogomez underlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation AT josemvinardell underlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation AT lidiacuestaberrio underlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation AT miguelarodriguezcarvajal underlyingprocessofearlyecologicalandgeneticdifferentiationinafacultativemutualisticsinorhizobiummelilotipopulation |
_version_ |
1718393995113005056 |