Obesity induced by neonatal overfeeding worsens airway hyperresponsiveness and inflammation.

<h4>Background</h4>Obesity is a risk factor for the development of certain respiratory diseases, and neonatal overfeeding results in an early onset of obesity in adulthood. However, the influence of neonatal overfeeding on respiratory diseases has rarely been studied. Therefore, this pap...

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Autores principales:	Zehui Ye, Ying Huang, Dan Liu, Xiaoyi Chen, Dongjuan Wang, Daochao Huang, Li Zhao, Xiaoqiu Xiao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d4c206ed2344691b2ee962b57df652d
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spelling	oai:doaj.org-article:9d4c206ed2344691b2ee962b57df652d2021-11-18T08:12:46ZObesity induced by neonatal overfeeding worsens airway hyperresponsiveness and inflammation.1932-620310.1371/journal.pone.0047013https://doaj.org/article/9d4c206ed2344691b2ee962b57df652d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23056561/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Obesity is a risk factor for the development of certain respiratory diseases, and neonatal overfeeding results in an early onset of obesity in adulthood. However, the influence of neonatal overfeeding on respiratory diseases has rarely been studied. Therefore, this paper is aimed at investigating the effect of neonatal overfeeding on airway responsiveness and inflammation.<h4>Methodology/principal findings</h4>The neonatal overfeeding was induced by reducing litter size to three pups per litter (small litter, SL) in contrast to the normal litter size with ten pups per litter (NL) on postnatal day 3 (P3) in male ICR mice. On P21, mice were weaned to standard chow diet. Airway responsiveness to methacholine was measured either on P21 or P150. Total and classified inflammatory cells in bronchoalveolar lavage fluid (BALF) were counted, lung inflammatory cells were evaluated through staining with hematoxylin & eosin and F4/80 immunohistochemistry; lung fibrosis was evaluated through staining with Masson and α-SAM immunohistochemistry. Leptin levels in serum were measured by RIA; TNF-α levels in serum and BALF were quantified by ELISA; mRNA levels of TNF-α, CTGF and TGF-β1 in lung tissues were measured using real-time PCR. Mice from SL exhibited accelerated body weight gain, impaired glucose tolerance and hyperleptinemia. Enhanced airway responsiveness to methacholine was observed in SL mice on P150, but not on P21. Pulmonary inflammation was evident in SL mice on P150, as reflected by inflammatory cells especially macrophages around bronchi and interstitium. BALF and serum TNF-α levels and lung TNF-α mRNA expression were significantly increased in SL mice on P150. More collagen accumulated surrounding the bronchi on P150; lung mRNA levels of TGF-β1 and CTGF were also increased on P150.<h4>Conclusion</h4>In addition to inducing a variety of metabolic defects, neonatal overfeeding enhanced lung inflammation, which may lead to airway remodeling and airway hyperresponsiveness in adulthood.Zehui YeYing HuangDan LiuXiaoyi ChenDongjuan WangDaochao HuangLi ZhaoXiaoqiu XiaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e47013 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zehui Ye Ying Huang Dan Liu Xiaoyi Chen Dongjuan Wang Daochao Huang Li Zhao Xiaoqiu Xiao Obesity induced by neonatal overfeeding worsens airway hyperresponsiveness and inflammation.
description	<h4>Background</h4>Obesity is a risk factor for the development of certain respiratory diseases, and neonatal overfeeding results in an early onset of obesity in adulthood. However, the influence of neonatal overfeeding on respiratory diseases has rarely been studied. Therefore, this paper is aimed at investigating the effect of neonatal overfeeding on airway responsiveness and inflammation.<h4>Methodology/principal findings</h4>The neonatal overfeeding was induced by reducing litter size to three pups per litter (small litter, SL) in contrast to the normal litter size with ten pups per litter (NL) on postnatal day 3 (P3) in male ICR mice. On P21, mice were weaned to standard chow diet. Airway responsiveness to methacholine was measured either on P21 or P150. Total and classified inflammatory cells in bronchoalveolar lavage fluid (BALF) were counted, lung inflammatory cells were evaluated through staining with hematoxylin & eosin and F4/80 immunohistochemistry; lung fibrosis was evaluated through staining with Masson and α-SAM immunohistochemistry. Leptin levels in serum were measured by RIA; TNF-α levels in serum and BALF were quantified by ELISA; mRNA levels of TNF-α, CTGF and TGF-β1 in lung tissues were measured using real-time PCR. Mice from SL exhibited accelerated body weight gain, impaired glucose tolerance and hyperleptinemia. Enhanced airway responsiveness to methacholine was observed in SL mice on P150, but not on P21. Pulmonary inflammation was evident in SL mice on P150, as reflected by inflammatory cells especially macrophages around bronchi and interstitium. BALF and serum TNF-α levels and lung TNF-α mRNA expression were significantly increased in SL mice on P150. More collagen accumulated surrounding the bronchi on P150; lung mRNA levels of TGF-β1 and CTGF were also increased on P150.<h4>Conclusion</h4>In addition to inducing a variety of metabolic defects, neonatal overfeeding enhanced lung inflammation, which may lead to airway remodeling and airway hyperresponsiveness in adulthood.
format	article
author	Zehui Ye Ying Huang Dan Liu Xiaoyi Chen Dongjuan Wang Daochao Huang Li Zhao Xiaoqiu Xiao
author_facet	Zehui Ye Ying Huang Dan Liu Xiaoyi Chen Dongjuan Wang Daochao Huang Li Zhao Xiaoqiu Xiao
author_sort	Zehui Ye
title	Obesity induced by neonatal overfeeding worsens airway hyperresponsiveness and inflammation.
title_short	Obesity induced by neonatal overfeeding worsens airway hyperresponsiveness and inflammation.
title_full	Obesity induced by neonatal overfeeding worsens airway hyperresponsiveness and inflammation.
title_fullStr	Obesity induced by neonatal overfeeding worsens airway hyperresponsiveness and inflammation.
title_full_unstemmed	Obesity induced by neonatal overfeeding worsens airway hyperresponsiveness and inflammation.
title_sort	obesity induced by neonatal overfeeding worsens airway hyperresponsiveness and inflammation.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/9d4c206ed2344691b2ee962b57df652d
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Obesity induced by neonatal overfeeding worsens airway hyperresponsiveness and inflammation.

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