Evaluation of interface quality in organ-cultured lamellar corneal transplants

Armin Wolf,1,* Burkhard&nbsp;von Jagow,2,* Daniel Kook,1 Elisabeth M Messmer,1 Carlo A Lackerbauer,1 Anselm Kampik,1 Thomas Kohnen,2 Martin Grueterich1 1Department of Ophthalmology, Ludwig-Maximilians-Universit&auml;t, M&uuml;nchen, 2Department of Ophthalmology, Johann Wolfga...

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Autores principales:	Wolf A, von Jagow B, Kook D, Messmer EM, Lackerbauer CA, Kampik A, Kohnen T, Grueterich M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/9d4e74426aab4831b0abfcff0796dd78
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spelling	oai:doaj.org-article:9d4e74426aab4831b0abfcff0796dd782021-12-02T01:40:28ZEvaluation of interface quality in organ-cultured lamellar corneal transplants1177-54671177-5483https://doaj.org/article/9d4e74426aab4831b0abfcff0796dd782012-07-01T00:00:00Zhttp://www.dovepress.com/evaluation-of-interface-quality-in-organ-cultured-lamellar-corneal-tra-a10425https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Armin Wolf,1,* Burkhard&nbsp;von Jagow,2,* Daniel Kook,1 Elisabeth M Messmer,1 Carlo A Lackerbauer,1 Anselm Kampik,1 Thomas Kohnen,2 Martin Grueterich1 1Department of Ophthalmology, Ludwig-Maximilians-Universit&auml;t, M&uuml;nchen, 2Department of Ophthalmology, Johann Wolfgang Goethe-Universit&auml;t, Frankfurt am Main, Germany *These authors have contributed equallyBackground: With increasing numbers of lamellar keratoplasties, eye banks are challenged to deliver precut lamellar donor tissue. In Europe, the most common technique of corneal storage is organ culture which requires a deswelling process before surgical processing. The aim of this study was to investigate the influence of different deswelling times on the cutting plane quality after microkeratome-assisted lamellar dissection.Methods: Eight paired donor corneas (16 specimens) not suitable for transplantation were organ cultured under standard conditions at the Eye Bank of the Ludwig-Maximilians Universit&auml;t, Munich, Germany. Pairs of corneal buttons were analyzed during the deswelling process in dextrane-containing medium. While one cornea was cut at an early time point during the deswelling process and put back into deswelling medium thereafter, the partner cornea was completely deswollen and dissected after 72 hours. Specimens were then further processed for scanning electron microscopy. Surface quality was assessed both digitally using Scanning Probe Imaging Processing software, and manually by three blinded graders.Results: The corneal buttons processed at the beginning of the deswelling process had a smoother surface when compared to the partner cornea that was cut at the end of the deswelling process. In our setting, no relevant difference was detectable between manual and automated microkeratome dissection.Conclusion: For lamellar keratoplasty, organ-cultured corneas should be processed at an early stage during the deswelling process. We interpret the smoother dissection plane during early deswelling as a result of mechanical properties in a highly hydrated cornea.Keywords: keratoplasty, deswelling, corneas, microkeratomeWolf Avon Jagow BKook DMessmer EMLackerbauer CAKampik AKohnen TGrueterich MDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2012, Iss default, Pp 967-972 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Wolf A von Jagow B Kook D Messmer EM Lackerbauer CA Kampik A Kohnen T Grueterich M Evaluation of interface quality in organ-cultured lamellar corneal transplants
description	Armin Wolf,1,* Burkhard&nbsp;von Jagow,2,* Daniel Kook,1 Elisabeth M Messmer,1 Carlo A Lackerbauer,1 Anselm Kampik,1 Thomas Kohnen,2 Martin Grueterich1 1Department of Ophthalmology, Ludwig-Maximilians-Universit&auml;t, M&uuml;nchen, 2Department of Ophthalmology, Johann Wolfgang Goethe-Universit&auml;t, Frankfurt am Main, Germany *These authors have contributed equallyBackground: With increasing numbers of lamellar keratoplasties, eye banks are challenged to deliver precut lamellar donor tissue. In Europe, the most common technique of corneal storage is organ culture which requires a deswelling process before surgical processing. The aim of this study was to investigate the influence of different deswelling times on the cutting plane quality after microkeratome-assisted lamellar dissection.Methods: Eight paired donor corneas (16 specimens) not suitable for transplantation were organ cultured under standard conditions at the Eye Bank of the Ludwig-Maximilians Universit&auml;t, Munich, Germany. Pairs of corneal buttons were analyzed during the deswelling process in dextrane-containing medium. While one cornea was cut at an early time point during the deswelling process and put back into deswelling medium thereafter, the partner cornea was completely deswollen and dissected after 72 hours. Specimens were then further processed for scanning electron microscopy. Surface quality was assessed both digitally using Scanning Probe Imaging Processing software, and manually by three blinded graders.Results: The corneal buttons processed at the beginning of the deswelling process had a smoother surface when compared to the partner cornea that was cut at the end of the deswelling process. In our setting, no relevant difference was detectable between manual and automated microkeratome dissection.Conclusion: For lamellar keratoplasty, organ-cultured corneas should be processed at an early stage during the deswelling process. We interpret the smoother dissection plane during early deswelling as a result of mechanical properties in a highly hydrated cornea.Keywords: keratoplasty, deswelling, corneas, microkeratome
format	article
author	Wolf A von Jagow B Kook D Messmer EM Lackerbauer CA Kampik A Kohnen T Grueterich M
author_facet	Wolf A von Jagow B Kook D Messmer EM Lackerbauer CA Kampik A Kohnen T Grueterich M
author_sort	Wolf A
title	Evaluation of interface quality in organ-cultured lamellar corneal transplants
title_short	Evaluation of interface quality in organ-cultured lamellar corneal transplants
title_full	Evaluation of interface quality in organ-cultured lamellar corneal transplants
title_fullStr	Evaluation of interface quality in organ-cultured lamellar corneal transplants
title_full_unstemmed	Evaluation of interface quality in organ-cultured lamellar corneal transplants
title_sort	evaluation of interface quality in organ-cultured lamellar corneal transplants
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/9d4e74426aab4831b0abfcff0796dd78
work_keys_str_mv	AT wolfa evaluationofinterfacequalityinorganculturedlamellarcornealtransplants AT vonjagowb evaluationofinterfacequalityinorganculturedlamellarcornealtransplants AT kookd evaluationofinterfacequalityinorganculturedlamellarcornealtransplants AT messmerem evaluationofinterfacequalityinorganculturedlamellarcornealtransplants AT lackerbauerca evaluationofinterfacequalityinorganculturedlamellarcornealtransplants AT kampika evaluationofinterfacequalityinorganculturedlamellarcornealtransplants AT kohnent evaluationofinterfacequalityinorganculturedlamellarcornealtransplants AT grueterichm evaluationofinterfacequalityinorganculturedlamellarcornealtransplants
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Evaluation of interface quality in organ-cultured lamellar corneal transplants

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