The Effect of Aqueous Extract of Trachyspermum ammi Seeds and Ibuprofen on Inflammatory Gene Expression in the Cartilage Tissue of Rats with Collagen-Induced Arthritis

Mohsen Korani, 1, 2 Mohammadnabi Jamshidi 2 1Chemical Injuries Research Center, System Biology and Poisoning Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran; 2Department of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, IranCorresponden...

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Autores principales:	Korani M, Jamshidi M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	rheumatoid arthritis trachyspermum ammi ibuprofen cyclooxygenase-2 nitric oxide synthase type ii Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/9d5956c38c544f5797ef5644c524309e
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spelling	oai:doaj.org-article:9d5956c38c544f5797ef5644c524309e2021-12-02T09:17:04ZThe Effect of Aqueous Extract of Trachyspermum ammi Seeds and Ibuprofen on Inflammatory Gene Expression in the Cartilage Tissue of Rats with Collagen-Induced Arthritis1178-7031https://doaj.org/article/9d5956c38c544f5797ef5644c524309e2020-02-01T00:00:00Zhttps://www.dovepress.com/the-effect-of-aqueous-extract-of-trachyspermum-ammi-seeds-and-ibuprofe-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Mohsen Korani, 1, 2 Mohammadnabi Jamshidi 2 1Chemical Injuries Research Center, System Biology and Poisoning Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran; 2Department of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, IranCorrespondence: Mohsen Korani Mollasadra Avenue, Vanak Square, Tehran, IranTel +98 21 82483417Email mohsenkorani@gmail.comBackground and Objectives: Rheumatoid arthritis (RA) is an inflammatory disease treated with nonsteroidal anti–inflammatory drugs that have different side effects. One of the plants used for this purpose in the traditional medicine is Trachyspermum ammi. The present study aimed at investigating the anti–inflammatory effect of this plant on type II collagen-induced arthritis (CIA) in Wistar rats.Materials and Methods: The study was performed on 35 male Wistar rats. Seven rats were considered as the healthy control group (normal group), and CIA was stablished in the rest. The rats with a model of inflammatory arthritis were divided into four groups. One group did not receive any treatment and three groups were treated orally with ibuprofen (15 mg/kg), aqueous extract of the T. ammi seeds (100 mg/kg), or their combination for 30 days. The effect of different treatments was investigated on the paw thickness, arthritis score, and mRNA level of COX2 and iNOS genes.Results: CIA increased paw thickness, arthritis score, and COX2 and iNOS mRNA levels compared to those of the normal group. Treatment with ibuprofen and aqueous extract alone or in combination reduced the studied variables. Reduction in the paw thickness, arthritis score, and iNOS mRNA level was more in the ibuprofen-treated group than the T. ammi extract-treated group, but treatment with T. ammi extract reduced COX2 mRNA level more than ibuprofen.Conclusion: It seems that the aqueous extract of T. ammi can be used alone or in combination with ibuprofen to treat RA.Keywords: rheumatoid arthritis, Trachyspermum ammi, ibuprofen, cyclooxygenase-2, nitric oxide synthase type IIKorani MJamshidi MDove Medical Pressarticlerheumatoid arthritistrachyspermum ammiibuprofencyclooxygenase-2nitric oxide synthase type iiPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 13, Pp 133-139 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	rheumatoid arthritis trachyspermum ammi ibuprofen cyclooxygenase-2 nitric oxide synthase type ii Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	rheumatoid arthritis trachyspermum ammi ibuprofen cyclooxygenase-2 nitric oxide synthase type ii Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Korani M Jamshidi M The Effect of Aqueous Extract of Trachyspermum ammi Seeds and Ibuprofen on Inflammatory Gene Expression in the Cartilage Tissue of Rats with Collagen-Induced Arthritis
description	Mohsen Korani, 1, 2 Mohammadnabi Jamshidi 2 1Chemical Injuries Research Center, System Biology and Poisoning Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran; 2Department of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, IranCorrespondence: Mohsen Korani Mollasadra Avenue, Vanak Square, Tehran, IranTel +98 21 82483417Email mohsenkorani@gmail.comBackground and Objectives: Rheumatoid arthritis (RA) is an inflammatory disease treated with nonsteroidal anti–inflammatory drugs that have different side effects. One of the plants used for this purpose in the traditional medicine is Trachyspermum ammi. The present study aimed at investigating the anti–inflammatory effect of this plant on type II collagen-induced arthritis (CIA) in Wistar rats.Materials and Methods: The study was performed on 35 male Wistar rats. Seven rats were considered as the healthy control group (normal group), and CIA was stablished in the rest. The rats with a model of inflammatory arthritis were divided into four groups. One group did not receive any treatment and three groups were treated orally with ibuprofen (15 mg/kg), aqueous extract of the T. ammi seeds (100 mg/kg), or their combination for 30 days. The effect of different treatments was investigated on the paw thickness, arthritis score, and mRNA level of COX2 and iNOS genes.Results: CIA increased paw thickness, arthritis score, and COX2 and iNOS mRNA levels compared to those of the normal group. Treatment with ibuprofen and aqueous extract alone or in combination reduced the studied variables. Reduction in the paw thickness, arthritis score, and iNOS mRNA level was more in the ibuprofen-treated group than the T. ammi extract-treated group, but treatment with T. ammi extract reduced COX2 mRNA level more than ibuprofen.Conclusion: It seems that the aqueous extract of T. ammi can be used alone or in combination with ibuprofen to treat RA.Keywords: rheumatoid arthritis, Trachyspermum ammi, ibuprofen, cyclooxygenase-2, nitric oxide synthase type II
format	article
author	Korani M Jamshidi M
author_facet	Korani M Jamshidi M
author_sort	Korani M
title	The Effect of Aqueous Extract of Trachyspermum ammi Seeds and Ibuprofen on Inflammatory Gene Expression in the Cartilage Tissue of Rats with Collagen-Induced Arthritis
title_short	The Effect of Aqueous Extract of Trachyspermum ammi Seeds and Ibuprofen on Inflammatory Gene Expression in the Cartilage Tissue of Rats with Collagen-Induced Arthritis
title_full	The Effect of Aqueous Extract of Trachyspermum ammi Seeds and Ibuprofen on Inflammatory Gene Expression in the Cartilage Tissue of Rats with Collagen-Induced Arthritis
title_fullStr	The Effect of Aqueous Extract of Trachyspermum ammi Seeds and Ibuprofen on Inflammatory Gene Expression in the Cartilage Tissue of Rats with Collagen-Induced Arthritis
title_full_unstemmed	The Effect of Aqueous Extract of Trachyspermum ammi Seeds and Ibuprofen on Inflammatory Gene Expression in the Cartilage Tissue of Rats with Collagen-Induced Arthritis
title_sort	effect of aqueous extract of trachyspermum ammi seeds and ibuprofen on inflammatory gene expression in the cartilage tissue of rats with collagen-induced arthritis
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/9d5956c38c544f5797ef5644c524309e
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The Effect of Aqueous Extract of Trachyspermum ammi Seeds and Ibuprofen on Inflammatory Gene Expression in the Cartilage Tissue of Rats with Collagen-Induced Arthritis

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