Assessment of prognostic value of tissue inhibitors of metalloproteinase 3 (TIMP3) protein in ovarian cancer

Background: TIMP3 is a multifunctional proteolytic enzyme belonging to TIMPs family and acts as a potent inhibitor of matrix metalloproteinases (MMPs). TIMP3 possesses a tumor suppresive function by directly promoting tumor cell apoptosis, preventing angiogenesis and extracellular matrix remodelling...

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Autores principales:	Sahar Hakamy, Mourad Assidi, Mohammad A. Jafri, Taoufik Nedjadi, Heba Alkhatabi, Abrar Al-Qahtani, Jaudah Al-Maghrabi, Khalid Sait, Mohammed Al-Qahtani, Abdelbaset Buhmeida, Adeel Chaudhary
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	ovarian cancer timp3 expression ihc tma prognosis Medicine R
Acceso en línea:	https://doaj.org/article/9d6d258a7e444be9b787b41ab7c134b5
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spelling	oai:doaj.org-article:9d6d258a7e444be9b787b41ab7c134b52021-11-26T11:19:48ZAssessment of prognostic value of tissue inhibitors of metalloproteinase 3 (TIMP3) protein in ovarian cancer1993-28201819-635710.1080/19932820.2021.1937866https://doaj.org/article/9d6d258a7e444be9b787b41ab7c134b52021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/19932820.2021.1937866https://doaj.org/toc/1993-2820https://doaj.org/toc/1819-6357Background: TIMP3 is a multifunctional proteolytic enzyme belonging to TIMPs family and acts as a potent inhibitor of matrix metalloproteinases (MMPs). TIMP3 possesses a tumor suppresive function by directly promoting tumor cell apoptosis, preventing angiogenesis and extracellular matrix remodelling. The lower expression of TIMP3 was associated with poor prognosis and overall survival in various cancer types. The aim of this study was to evaluate the association of TIMP3 protein expression with ovarian cancer (OC) clinicopathological features and survival outcomes.Patients and Methods: One hundred forty four of OC FFPE samples were collected from King Abdulaziz University Hospital, Saudi Arabia and constructed in tissue microarray (TMA) slides. Automated Ventana immunostainer platform was used to evaluate TIMP3 protein expression patterns.Results: The study showed that TIMP3 exhibits cytoplasmic localisation. This TIMP3 protein expression was not associated with age, tumor size and the involvement of lymph nodes (p > 0.05). However, it was significantly correlated with tumor stage (p < 0.05) and borderline significant with endpoint status (p = 0.07). Interestingly, the Kaplan-Meier analysis of disease specific survival (DSS) outcomes showed a significant association (p = 0.02, log rank) between OC patients with higher TIMP3 expression compared to those with lower expression. In fact, OC patients with high TIMP3 expression had longer survivals. Multivariate Cox’s regression analysis suggests that low TIMP3 protein expression pattern is an independent poor survival marker (p = 0.025).Conclusion: Cytoplasmic TIMP3 protein expression could be used as a good prognosticator to stratify poorly prognostic OC patients in order to personlaize their disease management.Sahar HakamyMourad AssidiMohammad A. JafriTaoufik NedjadiHeba AlkhatabiAbrar Al-QahtaniJaudah Al-MaghrabiKhalid SaitMohammed Al-QahtaniAbdelbaset BuhmeidaAdeel ChaudharyTaylor & Francis Grouparticleovarian cancertimp3 expressionihctmaprognosisMedicineRENLibyan Journal of Medicine, Vol 16, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ovarian cancer timp3 expression ihc tma prognosis Medicine R
spellingShingle	ovarian cancer timp3 expression ihc tma prognosis Medicine R Sahar Hakamy Mourad Assidi Mohammad A. Jafri Taoufik Nedjadi Heba Alkhatabi Abrar Al-Qahtani Jaudah Al-Maghrabi Khalid Sait Mohammed Al-Qahtani Abdelbaset Buhmeida Adeel Chaudhary Assessment of prognostic value of tissue inhibitors of metalloproteinase 3 (TIMP3) protein in ovarian cancer
description	Background: TIMP3 is a multifunctional proteolytic enzyme belonging to TIMPs family and acts as a potent inhibitor of matrix metalloproteinases (MMPs). TIMP3 possesses a tumor suppresive function by directly promoting tumor cell apoptosis, preventing angiogenesis and extracellular matrix remodelling. The lower expression of TIMP3 was associated with poor prognosis and overall survival in various cancer types. The aim of this study was to evaluate the association of TIMP3 protein expression with ovarian cancer (OC) clinicopathological features and survival outcomes.Patients and Methods: One hundred forty four of OC FFPE samples were collected from King Abdulaziz University Hospital, Saudi Arabia and constructed in tissue microarray (TMA) slides. Automated Ventana immunostainer platform was used to evaluate TIMP3 protein expression patterns.Results: The study showed that TIMP3 exhibits cytoplasmic localisation. This TIMP3 protein expression was not associated with age, tumor size and the involvement of lymph nodes (p > 0.05). However, it was significantly correlated with tumor stage (p < 0.05) and borderline significant with endpoint status (p = 0.07). Interestingly, the Kaplan-Meier analysis of disease specific survival (DSS) outcomes showed a significant association (p = 0.02, log rank) between OC patients with higher TIMP3 expression compared to those with lower expression. In fact, OC patients with high TIMP3 expression had longer survivals. Multivariate Cox’s regression analysis suggests that low TIMP3 protein expression pattern is an independent poor survival marker (p = 0.025).Conclusion: Cytoplasmic TIMP3 protein expression could be used as a good prognosticator to stratify poorly prognostic OC patients in order to personlaize their disease management.
format	article
author	Sahar Hakamy Mourad Assidi Mohammad A. Jafri Taoufik Nedjadi Heba Alkhatabi Abrar Al-Qahtani Jaudah Al-Maghrabi Khalid Sait Mohammed Al-Qahtani Abdelbaset Buhmeida Adeel Chaudhary
author_facet	Sahar Hakamy Mourad Assidi Mohammad A. Jafri Taoufik Nedjadi Heba Alkhatabi Abrar Al-Qahtani Jaudah Al-Maghrabi Khalid Sait Mohammed Al-Qahtani Abdelbaset Buhmeida Adeel Chaudhary
author_sort	Sahar Hakamy
title	Assessment of prognostic value of tissue inhibitors of metalloproteinase 3 (TIMP3) protein in ovarian cancer
title_short	Assessment of prognostic value of tissue inhibitors of metalloproteinase 3 (TIMP3) protein in ovarian cancer
title_full	Assessment of prognostic value of tissue inhibitors of metalloproteinase 3 (TIMP3) protein in ovarian cancer
title_fullStr	Assessment of prognostic value of tissue inhibitors of metalloproteinase 3 (TIMP3) protein in ovarian cancer
title_full_unstemmed	Assessment of prognostic value of tissue inhibitors of metalloproteinase 3 (TIMP3) protein in ovarian cancer
title_sort	assessment of prognostic value of tissue inhibitors of metalloproteinase 3 (timp3) protein in ovarian cancer
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/9d6d258a7e444be9b787b41ab7c134b5
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Assessment of prognostic value of tissue inhibitors of metalloproteinase 3 (TIMP3) protein in ovarian cancer

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