TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer

Abstract Previously, we cloned a new gene termed ‘tongue cancer resistance-associated protein 1’ (TCRP1), which modulates tumorigenesis, enhances cisplatin (cDDP) resistance in cancers, and may be a potential target for reversing drug resistance. However, the mechanisms for regulating TCRP1 expressi...

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Autores principales:	Xiaoting Jia, Zhijie Zhang, Kai Luo, Guopei Zheng, Minying Lu, Ying Song, Hao Liu, Huisi Qiu, Zhimin He
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d6d93dabe994a2ba343e17400ab6d6a
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spelling	oai:doaj.org-article:9d6d93dabe994a2ba343e17400ab6d6a2021-12-02T16:07:05ZTCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer10.1038/s41598-017-03763-02045-2322https://doaj.org/article/9d6d93dabe994a2ba343e17400ab6d6a2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03763-0https://doaj.org/toc/2045-2322Abstract Previously, we cloned a new gene termed ‘tongue cancer resistance-associated protein 1’ (TCRP1), which modulates tumorigenesis, enhances cisplatin (cDDP) resistance in cancers, and may be a potential target for reversing drug resistance. However, the mechanisms for regulating TCRP1 expression remain unclear. Herein, we combined bioinformatics analysis with luciferase reporter assay and ChIP assay to determine that c-Myc could directly bind to TCRP1 promoter to upregulate its expression. TCRP1 upregulation in multidrug resistant tongue cancer cells (Tca8113/PYM) and cisplatin-resistant A549 lung cancer cells (A549/DDP) was accompanied by c-Myc upregulation, compared to respective parental cells. In tongue and lung cancer cells, siRNA-mediated knockdown of c-Myc led to decrease TCRP1 expression, whereas overexpression c-Myc did the opposite. Moreover, TCRP1 knockdown attenuated chemoresistance resulting from c-Myc overexpression, but TCRP1 overexpression impaired the effect of c-Myc knockdown on chemosensitivity. Additionally, in both human tongue and lung cancer tissues, c-Myc protein expression positively correlated with TCRP1 protein expression and these protein levels were associated with worse prognosis for patients. Combined, these findings suggest that c-Myc could transcriptionally regulate TCRP1 in cell lines and clinical samples and identified the c-Myc-TCRP1 axis as a negative biomarker of prognosis in tongue and lung cancers.Xiaoting JiaZhijie ZhangKai LuoGuopei ZhengMinying LuYing SongHao LiuHuisi QiuZhimin HeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoting Jia Zhijie Zhang Kai Luo Guopei Zheng Minying Lu Ying Song Hao Liu Huisi Qiu Zhimin He TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer
description	Abstract Previously, we cloned a new gene termed ‘tongue cancer resistance-associated protein 1’ (TCRP1), which modulates tumorigenesis, enhances cisplatin (cDDP) resistance in cancers, and may be a potential target for reversing drug resistance. However, the mechanisms for regulating TCRP1 expression remain unclear. Herein, we combined bioinformatics analysis with luciferase reporter assay and ChIP assay to determine that c-Myc could directly bind to TCRP1 promoter to upregulate its expression. TCRP1 upregulation in multidrug resistant tongue cancer cells (Tca8113/PYM) and cisplatin-resistant A549 lung cancer cells (A549/DDP) was accompanied by c-Myc upregulation, compared to respective parental cells. In tongue and lung cancer cells, siRNA-mediated knockdown of c-Myc led to decrease TCRP1 expression, whereas overexpression c-Myc did the opposite. Moreover, TCRP1 knockdown attenuated chemoresistance resulting from c-Myc overexpression, but TCRP1 overexpression impaired the effect of c-Myc knockdown on chemosensitivity. Additionally, in both human tongue and lung cancer tissues, c-Myc protein expression positively correlated with TCRP1 protein expression and these protein levels were associated with worse prognosis for patients. Combined, these findings suggest that c-Myc could transcriptionally regulate TCRP1 in cell lines and clinical samples and identified the c-Myc-TCRP1 axis as a negative biomarker of prognosis in tongue and lung cancers.
format	article
author	Xiaoting Jia Zhijie Zhang Kai Luo Guopei Zheng Minying Lu Ying Song Hao Liu Huisi Qiu Zhimin He
author_facet	Xiaoting Jia Zhijie Zhang Kai Luo Guopei Zheng Minying Lu Ying Song Hao Liu Huisi Qiu Zhimin He
author_sort	Xiaoting Jia
title	TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer
title_short	TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer
title_full	TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer
title_fullStr	TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer
title_full_unstemmed	TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer
title_sort	tcrp1 transcriptionally regulated by c-myc confers cancer chemoresistance in tongue and lung cancer
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9d6d93dabe994a2ba343e17400ab6d6a
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TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer

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