Methods for assessment of body tissue composition as an indication of the energetic status in bivalve populations: A comparison of biochemical and elemental analysis

Elemental (CHN) and proximate biochemical compositions of body tissues are useful tools among the ecological indices most commonly used in evaluation of the energetic status of animal populations. Gnaiger and Bitterlich (1984) supplied procedures for the interconversion between both these measuremen...

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Autores principales:	Kristina Arranz, Iñaki Urrutxurtu, Daniel Prieto, Irrintzi Ibarrola, Miren Bego Urrutia, Enrique Navarro
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Body tissues Biochemical composition Elemental analysis Bivalve mollusks Genus Ruditapes Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/9d769550c93c4c279058fffc3dd0170e
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spelling	oai:doaj.org-article:9d769550c93c4c279058fffc3dd0170e2021-12-01T04:34:18ZMethods for assessment of body tissue composition as an indication of the energetic status in bivalve populations: A comparison of biochemical and elemental analysis1470-160X10.1016/j.ecolind.2020.107074https://doaj.org/article/9d769550c93c4c279058fffc3dd0170e2021-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1470160X2031013Xhttps://doaj.org/toc/1470-160XElemental (CHN) and proximate biochemical compositions of body tissues are useful tools among the ecological indices most commonly used in evaluation of the energetic status of animal populations. Gnaiger and Bitterlich (1984) supplied procedures for the interconversion between both these measurements based on stoichiometric relationships, that were further tested using gut contents and body tissue samples of freshwater fishes. Despite a lack of validation studies, the reported conversion factors have been broadly applied in the field of body composition analysis of marine invertebrates, especially bivalve mollusks. The aim of this study was to test the applicability of those conversion equivalents in analysis of the body tissues of two congeneric clam species (Ruditapes decussatus and Ruditapes philippinarum). To this end, proximate biochemical composition, as analyzed by fractionated extraction of tissues samples and quantification using spectrophotometric methods, was converted to elemental composition, and the resulting figures were compared with those of direct CHN analysis. The results of this comparison indicate good agreement within the ranges reported, provided that ninhydrin positive substances (NPS) are incorporated in the biochemical composition analyses. The magnitude of the nonprotein N component in bivalve tissues appears to complicate the reverse computation of biochemical components from elemental composition because no accurate estimation of proteins from N contents might be possible. Additionally, a specific correction of residual water in dried samples of bivalve tissues for CHN analysis should be applied. The absence of broad differences found between species reflects the morphological, evolutionary and functional proximity between them, whereas tissue differences display the differential role that each organ plays in the organism, although other sources of variability such as diet and sex should be addressed in future research.Kristina ArranzIñaki UrrutxurtuDaniel PrietoIrrintzi IbarrolaMiren Bego UrrutiaEnrique NavarroElsevierarticleBody tissuesBiochemical compositionElemental analysisBivalve mollusksGenus RuditapesEcologyQH540-549.5ENEcological Indicators, Vol 121, Iss , Pp 107074- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Body tissues Biochemical composition Elemental analysis Bivalve mollusks Genus Ruditapes Ecology QH540-549.5
spellingShingle	Body tissues Biochemical composition Elemental analysis Bivalve mollusks Genus Ruditapes Ecology QH540-549.5 Kristina Arranz Iñaki Urrutxurtu Daniel Prieto Irrintzi Ibarrola Miren Bego Urrutia Enrique Navarro Methods for assessment of body tissue composition as an indication of the energetic status in bivalve populations: A comparison of biochemical and elemental analysis
description	Elemental (CHN) and proximate biochemical compositions of body tissues are useful tools among the ecological indices most commonly used in evaluation of the energetic status of animal populations. Gnaiger and Bitterlich (1984) supplied procedures for the interconversion between both these measurements based on stoichiometric relationships, that were further tested using gut contents and body tissue samples of freshwater fishes. Despite a lack of validation studies, the reported conversion factors have been broadly applied in the field of body composition analysis of marine invertebrates, especially bivalve mollusks. The aim of this study was to test the applicability of those conversion equivalents in analysis of the body tissues of two congeneric clam species (Ruditapes decussatus and Ruditapes philippinarum). To this end, proximate biochemical composition, as analyzed by fractionated extraction of tissues samples and quantification using spectrophotometric methods, was converted to elemental composition, and the resulting figures were compared with those of direct CHN analysis. The results of this comparison indicate good agreement within the ranges reported, provided that ninhydrin positive substances (NPS) are incorporated in the biochemical composition analyses. The magnitude of the nonprotein N component in bivalve tissues appears to complicate the reverse computation of biochemical components from elemental composition because no accurate estimation of proteins from N contents might be possible. Additionally, a specific correction of residual water in dried samples of bivalve tissues for CHN analysis should be applied. The absence of broad differences found between species reflects the morphological, evolutionary and functional proximity between them, whereas tissue differences display the differential role that each organ plays in the organism, although other sources of variability such as diet and sex should be addressed in future research.
format	article
author	Kristina Arranz Iñaki Urrutxurtu Daniel Prieto Irrintzi Ibarrola Miren Bego Urrutia Enrique Navarro
author_facet	Kristina Arranz Iñaki Urrutxurtu Daniel Prieto Irrintzi Ibarrola Miren Bego Urrutia Enrique Navarro
author_sort	Kristina Arranz
title	Methods for assessment of body tissue composition as an indication of the energetic status in bivalve populations: A comparison of biochemical and elemental analysis
title_short	Methods for assessment of body tissue composition as an indication of the energetic status in bivalve populations: A comparison of biochemical and elemental analysis
title_full	Methods for assessment of body tissue composition as an indication of the energetic status in bivalve populations: A comparison of biochemical and elemental analysis
title_fullStr	Methods for assessment of body tissue composition as an indication of the energetic status in bivalve populations: A comparison of biochemical and elemental analysis
title_full_unstemmed	Methods for assessment of body tissue composition as an indication of the energetic status in bivalve populations: A comparison of biochemical and elemental analysis
title_sort	methods for assessment of body tissue composition as an indication of the energetic status in bivalve populations: a comparison of biochemical and elemental analysis
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/9d769550c93c4c279058fffc3dd0170e
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Methods for assessment of body tissue composition as an indication of the energetic status in bivalve populations: A comparison of biochemical and elemental analysis

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