Improved Analytical Sensitivity of Lateral Flow Assay using Sponge for HBV Nucleic Acid Detection

Abstract Hepatitis B virus (HBV) infection is a serious public health problem, which can be transmitted through various routes (e.g., blood donation) and cause hepatitis, liver cirrhosis and liver cancer. Hence, it is necessary to do diagnostic screening for high-risk HBV patients in these transmiss...

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Autores principales:	Ruihua Tang, Hui Yang, Yan Gong, Zhi Liu, XiuJun Li, Ting Wen, ZhiGuo Qu, Sufeng Zhang, Qibing Mei, Feng Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d769ce52ba6418faf0b5420f20e5771
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spelling	oai:doaj.org-article:9d769ce52ba6418faf0b5420f20e57712021-12-02T15:05:29ZImproved Analytical Sensitivity of Lateral Flow Assay using Sponge for HBV Nucleic Acid Detection10.1038/s41598-017-01558-x2045-2322https://doaj.org/article/9d769ce52ba6418faf0b5420f20e57712017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01558-xhttps://doaj.org/toc/2045-2322Abstract Hepatitis B virus (HBV) infection is a serious public health problem, which can be transmitted through various routes (e.g., blood donation) and cause hepatitis, liver cirrhosis and liver cancer. Hence, it is necessary to do diagnostic screening for high-risk HBV patients in these transmission routes. Nowadays, protein-based technologies have been used for HBV testing, which however involve the issues of large sample volume, antibody instability and poor specificity. Nucleic acid hybridization-based lateral flow assay (LFA) holds great potential to address these limitations due to its low-cost, rapid, and simple features, but the poor analytical sensitivity of LFA restricts its application. In this study, we developed a low-cost, simple and easy-to-use method to improve analytical sensitivity by integrating sponge shunt into LFA to decrease the fluid flow rate. The thickness, length and hydrophobicity of the sponge shunt were sequentially optimized, and achieved 10-fold signal enhancement in nucleic acid testing of HBV as compared to the unmodified LFA. The enhancement was further confirmed by using HBV clinical samples, where we achieved the detection limit of 103 copies/ml as compared to 104 copies/ml in unmodified LFA. The improved LFA holds great potential for diseases diagnostics, food safety control and environment monitoring at point-of-care.Ruihua TangHui YangYan GongZhi LiuXiuJun LiTing WenZhiGuo QuSufeng ZhangQibing MeiFeng XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ruihua Tang Hui Yang Yan Gong Zhi Liu XiuJun Li Ting Wen ZhiGuo Qu Sufeng Zhang Qibing Mei Feng Xu Improved Analytical Sensitivity of Lateral Flow Assay using Sponge for HBV Nucleic Acid Detection
description	Abstract Hepatitis B virus (HBV) infection is a serious public health problem, which can be transmitted through various routes (e.g., blood donation) and cause hepatitis, liver cirrhosis and liver cancer. Hence, it is necessary to do diagnostic screening for high-risk HBV patients in these transmission routes. Nowadays, protein-based technologies have been used for HBV testing, which however involve the issues of large sample volume, antibody instability and poor specificity. Nucleic acid hybridization-based lateral flow assay (LFA) holds great potential to address these limitations due to its low-cost, rapid, and simple features, but the poor analytical sensitivity of LFA restricts its application. In this study, we developed a low-cost, simple and easy-to-use method to improve analytical sensitivity by integrating sponge shunt into LFA to decrease the fluid flow rate. The thickness, length and hydrophobicity of the sponge shunt were sequentially optimized, and achieved 10-fold signal enhancement in nucleic acid testing of HBV as compared to the unmodified LFA. The enhancement was further confirmed by using HBV clinical samples, where we achieved the detection limit of 103 copies/ml as compared to 104 copies/ml in unmodified LFA. The improved LFA holds great potential for diseases diagnostics, food safety control and environment monitoring at point-of-care.
format	article
author	Ruihua Tang Hui Yang Yan Gong Zhi Liu XiuJun Li Ting Wen ZhiGuo Qu Sufeng Zhang Qibing Mei Feng Xu
author_facet	Ruihua Tang Hui Yang Yan Gong Zhi Liu XiuJun Li Ting Wen ZhiGuo Qu Sufeng Zhang Qibing Mei Feng Xu
author_sort	Ruihua Tang
title	Improved Analytical Sensitivity of Lateral Flow Assay using Sponge for HBV Nucleic Acid Detection
title_short	Improved Analytical Sensitivity of Lateral Flow Assay using Sponge for HBV Nucleic Acid Detection
title_full	Improved Analytical Sensitivity of Lateral Flow Assay using Sponge for HBV Nucleic Acid Detection
title_fullStr	Improved Analytical Sensitivity of Lateral Flow Assay using Sponge for HBV Nucleic Acid Detection
title_full_unstemmed	Improved Analytical Sensitivity of Lateral Flow Assay using Sponge for HBV Nucleic Acid Detection
title_sort	improved analytical sensitivity of lateral flow assay using sponge for hbv nucleic acid detection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9d769ce52ba6418faf0b5420f20e5771
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_version_	1718388849181196288

Improved Analytical Sensitivity of Lateral Flow Assay using Sponge for HBV Nucleic Acid Detection

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