Quantitative [Fe]MRI of PSMA-targeted SPIONs specifically discriminates among prostate tumor cell types based on their PSMA expression levels

Laurel O Sillerud BRaIN Center, Department of Neurology, University of New Mexico School of Medicine, Albuquerque, NM, USA Abstract: We report the development, experimental verification, and application of a general theory called [Fe]MRI (pronounced fem-ree) for the non-invasive, quantita...

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Autor principal:	Sillerud LO
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	[Fe]MRI Prostate tumor cells prostate specific membrane antigen Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9d81835ad2ef47bca478905f406f4f5b
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spelling	oai:doaj.org-article:9d81835ad2ef47bca478905f406f4f5b2021-12-02T00:35:52ZQuantitative [Fe]MRI of PSMA-targeted SPIONs specifically discriminates among prostate tumor cell types based on their PSMA expression levels1178-2013https://doaj.org/article/9d81835ad2ef47bca478905f406f4f5b2016-01-01T00:00:00Zhttps://www.dovepress.com/quantitative-femri-of-psma-targeted-spions-specifically-discriminates--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Laurel O Sillerud BRaIN Center, Department of Neurology, University of New Mexico School of Medicine, Albuquerque, NM, USA Abstract: We report the development, experimental verification, and application of a general theory called [Fe]MRI (pronounced fem-ree) for the non-invasive, quantitative molecular magnetic resonance imaging (MRI) of added magnetic nanoparticles or other magnetic contrast agents in biological tissues and other sites. [Fe]MRI can easily be implemented on any MRI instrument, requiring only measurements of the background nuclear magnetic relaxation times (T1, T2) of the tissue of interest, injection of the magnetic particles, and the subsequent acquisition of a pair of T1-weighted and T2-weighted images. These images, converted into contrast images, are subtracted to yield a contrast difference image proportional to the absolute nanoparticle, iron concentration, ([Fe]) image. [Fe]MRI was validated with the samples of superparamagnetic iron oxide nanoparticles (SPIONs) both in agarose gels and bound to human prostate tumor cells. The [Fe]MRI measurement of the binding of anti-prostate specific membrane antigen (PSMA) conjugated SPIONs to PSMA-positive LNCaP and PSMA-negative DU145 cells in vitro allowed a facile discrimination among prostate tumor cell types based on their PSMA expression level. The low [Fe] detection limit of ~2 µM for SPIONs allows sensitive MRI of added iron at concentrations considerably below the US Food and Drug Administration’s human iron dosage guidelines (<90 µM, 5 mg/kg). Keywords: contrast difference, RT-PCR, flow cytometrySillerud LODove Medical Pressarticle[Fe]MRIProstate tumor cellsprostate specific membrane antigenMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 357-372 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	[Fe]MRI Prostate tumor cells prostate specific membrane antigen Medicine (General) R5-920
spellingShingle	[Fe]MRI Prostate tumor cells prostate specific membrane antigen Medicine (General) R5-920 Sillerud LO Quantitative [Fe]MRI of PSMA-targeted SPIONs specifically discriminates among prostate tumor cell types based on their PSMA expression levels
description	Laurel O Sillerud BRaIN Center, Department of Neurology, University of New Mexico School of Medicine, Albuquerque, NM, USA Abstract: We report the development, experimental verification, and application of a general theory called [Fe]MRI (pronounced fem-ree) for the non-invasive, quantitative molecular magnetic resonance imaging (MRI) of added magnetic nanoparticles or other magnetic contrast agents in biological tissues and other sites. [Fe]MRI can easily be implemented on any MRI instrument, requiring only measurements of the background nuclear magnetic relaxation times (T1, T2) of the tissue of interest, injection of the magnetic particles, and the subsequent acquisition of a pair of T1-weighted and T2-weighted images. These images, converted into contrast images, are subtracted to yield a contrast difference image proportional to the absolute nanoparticle, iron concentration, ([Fe]) image. [Fe]MRI was validated with the samples of superparamagnetic iron oxide nanoparticles (SPIONs) both in agarose gels and bound to human prostate tumor cells. The [Fe]MRI measurement of the binding of anti-prostate specific membrane antigen (PSMA) conjugated SPIONs to PSMA-positive LNCaP and PSMA-negative DU145 cells in vitro allowed a facile discrimination among prostate tumor cell types based on their PSMA expression level. The low [Fe] detection limit of ~2 µM for SPIONs allows sensitive MRI of added iron at concentrations considerably below the US Food and Drug Administration’s human iron dosage guidelines (<90 µM, 5 mg/kg). Keywords: contrast difference, RT-PCR, flow cytometry
format	article
author	Sillerud LO
author_facet	Sillerud LO
author_sort	Sillerud LO
title	Quantitative [Fe]MRI of PSMA-targeted SPIONs specifically discriminates among prostate tumor cell types based on their PSMA expression levels
title_short	Quantitative [Fe]MRI of PSMA-targeted SPIONs specifically discriminates among prostate tumor cell types based on their PSMA expression levels
title_full	Quantitative [Fe]MRI of PSMA-targeted SPIONs specifically discriminates among prostate tumor cell types based on their PSMA expression levels
title_fullStr	Quantitative [Fe]MRI of PSMA-targeted SPIONs specifically discriminates among prostate tumor cell types based on their PSMA expression levels
title_full_unstemmed	Quantitative [Fe]MRI of PSMA-targeted SPIONs specifically discriminates among prostate tumor cell types based on their PSMA expression levels
title_sort	quantitative [fe]mri of psma-targeted spions specifically discriminates among prostate tumor cell types based on their psma expression levels
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/9d81835ad2ef47bca478905f406f4f5b
work_keys_str_mv	AT sillerudlo quantitativefemriofpsmatargetedspionsspecificallydiscriminatesamongprostatetumorcelltypesbasedontheirpsmaexpressionlevels
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Quantitative [Fe]MRI of PSMA-targeted SPIONs specifically discriminates among prostate tumor cell types based on their PSMA expression levels

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