A multiomics approach to identify host-microbe alterations associated with infection severity in diabetic foot infections: a pilot study

Abstract Diabetic foot infections (DFIs) are a major cause of hospitalization and can lead to lower extremity amputation. In this pilot study, we used a multiomics approach to explore the host–microbe complex within DFIs. We observed minimal differences in the overall microbial composition between P...

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Autores principales:	Michael Radzieta, Fatemah Sadeghpour-Heravi, Timothy J. Peters, Honghua Hu, Karen Vickery, Thomas Jeffries, Hugh G. Dickson, Saskia Schwarzer, Slade O. Jensen, Matthew Malone
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/9d872203a61e4bfe96312e7f76c0ee92
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spelling	oai:doaj.org-article:9d872203a61e4bfe96312e7f76c0ee922021-12-02T14:02:56ZA multiomics approach to identify host-microbe alterations associated with infection severity in diabetic foot infections: a pilot study10.1038/s41522-021-00202-x2055-5008https://doaj.org/article/9d872203a61e4bfe96312e7f76c0ee922021-03-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00202-xhttps://doaj.org/toc/2055-5008Abstract Diabetic foot infections (DFIs) are a major cause of hospitalization and can lead to lower extremity amputation. In this pilot study, we used a multiomics approach to explore the host–microbe complex within DFIs. We observed minimal differences in the overall microbial composition between PEDIS infection severities, however Staphylococcus aureus and Streptococcus genera were abundant and highly active in most mild to moderate DFIs. Further, we identified the significant enrichment of several virulence factors associated with infection pathogenicity belonging to both Staphylococcus aureus and Streptococcus. In severe DFIs, patients demonstrated a greater microbial diversity and differential gene expression demonstrated the enrichment of multispecies virulence genes suggestive of a complex polymicrobial infection. The host response in patients with severe DFIs was also significantly different as compared to mild to moderate DFIs. This was attributed to the enrichment of host genes associated with inflammation, acute phase response, cell stress and broad immune-related responses, while those associated with wound healing and myogenesis were significantly depleted.Michael RadzietaFatemah Sadeghpour-HeraviTimothy J. PetersHonghua HuKaren VickeryThomas JeffriesHugh G. DicksonSaskia SchwarzerSlade O. JensenMatthew MaloneNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Michael Radzieta Fatemah Sadeghpour-Heravi Timothy J. Peters Honghua Hu Karen Vickery Thomas Jeffries Hugh G. Dickson Saskia Schwarzer Slade O. Jensen Matthew Malone A multiomics approach to identify host-microbe alterations associated with infection severity in diabetic foot infections: a pilot study
description	Abstract Diabetic foot infections (DFIs) are a major cause of hospitalization and can lead to lower extremity amputation. In this pilot study, we used a multiomics approach to explore the host–microbe complex within DFIs. We observed minimal differences in the overall microbial composition between PEDIS infection severities, however Staphylococcus aureus and Streptococcus genera were abundant and highly active in most mild to moderate DFIs. Further, we identified the significant enrichment of several virulence factors associated with infection pathogenicity belonging to both Staphylococcus aureus and Streptococcus. In severe DFIs, patients demonstrated a greater microbial diversity and differential gene expression demonstrated the enrichment of multispecies virulence genes suggestive of a complex polymicrobial infection. The host response in patients with severe DFIs was also significantly different as compared to mild to moderate DFIs. This was attributed to the enrichment of host genes associated with inflammation, acute phase response, cell stress and broad immune-related responses, while those associated with wound healing and myogenesis were significantly depleted.
format	article
author	Michael Radzieta Fatemah Sadeghpour-Heravi Timothy J. Peters Honghua Hu Karen Vickery Thomas Jeffries Hugh G. Dickson Saskia Schwarzer Slade O. Jensen Matthew Malone
author_facet	Michael Radzieta Fatemah Sadeghpour-Heravi Timothy J. Peters Honghua Hu Karen Vickery Thomas Jeffries Hugh G. Dickson Saskia Schwarzer Slade O. Jensen Matthew Malone
author_sort	Michael Radzieta
title	A multiomics approach to identify host-microbe alterations associated with infection severity in diabetic foot infections: a pilot study
title_short	A multiomics approach to identify host-microbe alterations associated with infection severity in diabetic foot infections: a pilot study
title_full	A multiomics approach to identify host-microbe alterations associated with infection severity in diabetic foot infections: a pilot study
title_fullStr	A multiomics approach to identify host-microbe alterations associated with infection severity in diabetic foot infections: a pilot study
title_full_unstemmed	A multiomics approach to identify host-microbe alterations associated with infection severity in diabetic foot infections: a pilot study
title_sort	multiomics approach to identify host-microbe alterations associated with infection severity in diabetic foot infections: a pilot study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9d872203a61e4bfe96312e7f76c0ee92
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A multiomics approach to identify host-microbe alterations associated with infection severity in diabetic foot infections: a pilot study

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