Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles

Abstract Fungal infections and toxicoses caused by insecticides may alter microbial communities and immune responses in the insect gut. We investigated the effects of Metarhizium robertsii fungus and avermectins on the midgut physiology of Colorado potato beetle larvae. We analyzed changes in the ba...

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Autores principales:	Vadim Yu. Kryukov, Ulyana Rotskaya, Olga Yaroslavtseva, Olga Polenogova, Natalia Kryukova, Yuriy Akhanaev, Anton Krivopalov, Tatyana Alikina, Yana L. Vorontsova, Irina Slepneva, Marsel Kabilov, Viktor V. Glupov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d8b9c71e189471d84351a94bcb05ec6
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spelling	oai:doaj.org-article:9d8b9c71e189471d84351a94bcb05ec62021-12-02T15:22:56ZFungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles10.1038/s41598-020-80565-x2045-2322https://doaj.org/article/9d8b9c71e189471d84351a94bcb05ec62021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80565-xhttps://doaj.org/toc/2045-2322Abstract Fungal infections and toxicoses caused by insecticides may alter microbial communities and immune responses in the insect gut. We investigated the effects of Metarhizium robertsii fungus and avermectins on the midgut physiology of Colorado potato beetle larvae. We analyzed changes in the bacterial community, immunity- and stress-related gene expression, reactive oxygen species (ROS) production, and detoxification enzyme activity in response to topical infection with the M. robertsii fungus, oral administration of avermectins, and a combination of the two treatments. Avermectin treatment led to a reduction in microbiota diversity and an enhancement in the abundance of enterobacteria, and these changes were followed by the downregulation of Stat and Hsp90, upregulation of transcription factors for the Toll and IMD pathways and activation of detoxification enzymes. Fungal infection also led to a decrease in microbiota diversity, although the changes in community structure were not significant, except for the enhancement of Serratia. Fungal infection decreased the production of ROS but did not affect the gene expression of the immune pathways. In the combined treatment, fungal infection inhibited the activation of detoxification enzymes and prevented the downregulation of the JAK-STAT pathway caused by avermectins. The results of this study suggest that fungal infection modulates physiological responses to avermectins and that fungal infection may increase avermectin toxicosis by blocking detoxification enzymes in the gut.Vadim Yu. KryukovUlyana RotskayaOlga YaroslavtsevaOlga PolenogovaNatalia KryukovaYuriy AkhanaevAnton KrivopalovTatyana AlikinaYana L. VorontsovaIrina SlepnevaMarsel KabilovViktor V. GlupovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vadim Yu. Kryukov Ulyana Rotskaya Olga Yaroslavtseva Olga Polenogova Natalia Kryukova Yuriy Akhanaev Anton Krivopalov Tatyana Alikina Yana L. Vorontsova Irina Slepneva Marsel Kabilov Viktor V. Glupov Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles
description	Abstract Fungal infections and toxicoses caused by insecticides may alter microbial communities and immune responses in the insect gut. We investigated the effects of Metarhizium robertsii fungus and avermectins on the midgut physiology of Colorado potato beetle larvae. We analyzed changes in the bacterial community, immunity- and stress-related gene expression, reactive oxygen species (ROS) production, and detoxification enzyme activity in response to topical infection with the M. robertsii fungus, oral administration of avermectins, and a combination of the two treatments. Avermectin treatment led to a reduction in microbiota diversity and an enhancement in the abundance of enterobacteria, and these changes were followed by the downregulation of Stat and Hsp90, upregulation of transcription factors for the Toll and IMD pathways and activation of detoxification enzymes. Fungal infection also led to a decrease in microbiota diversity, although the changes in community structure were not significant, except for the enhancement of Serratia. Fungal infection decreased the production of ROS but did not affect the gene expression of the immune pathways. In the combined treatment, fungal infection inhibited the activation of detoxification enzymes and prevented the downregulation of the JAK-STAT pathway caused by avermectins. The results of this study suggest that fungal infection modulates physiological responses to avermectins and that fungal infection may increase avermectin toxicosis by blocking detoxification enzymes in the gut.
format	article
author	Vadim Yu. Kryukov Ulyana Rotskaya Olga Yaroslavtseva Olga Polenogova Natalia Kryukova Yuriy Akhanaev Anton Krivopalov Tatyana Alikina Yana L. Vorontsova Irina Slepneva Marsel Kabilov Viktor V. Glupov
author_facet	Vadim Yu. Kryukov Ulyana Rotskaya Olga Yaroslavtseva Olga Polenogova Natalia Kryukova Yuriy Akhanaev Anton Krivopalov Tatyana Alikina Yana L. Vorontsova Irina Slepneva Marsel Kabilov Viktor V. Glupov
author_sort	Vadim Yu. Kryukov
title	Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles
title_short	Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles
title_full	Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles
title_fullStr	Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles
title_full_unstemmed	Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles
title_sort	fungus metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in colorado potato beetles
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9d8b9c71e189471d84351a94bcb05ec6
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Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles

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