Physicochemical Investigation of Biosynthesis of a Protein Coating on Glass That Promotes Mammalian Cell Growth Using <i>Lactobacillus rhamnosus</i> GG Bacteria

Glass surfaces, although the first to be used for culturing ex vivo adherent cells, are not the perfect substrates for this purpose. Today, plastics dominate these applications, but in light of the global trend to reduce the use of synthetic polymers, it is reasonable to consider a return to glass v...

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Autores principales:	Kamil Kaminski, Karolina Syrek, Joanna Grudzień, Magdalena Obloza, Monika Adamczyk, Grzegorz D. Sulka
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	glass coating <i>Lactobacillus rhamnosus</i> GG cell growth gold nanoparticles Engineering (General). Civil engineering (General) TA1-2040
Acceso en línea:	https://doaj.org/article/9d90dff745a7448991a8728393befeb4
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spelling	oai:doaj.org-article:9d90dff745a7448991a8728393befeb42021-11-25T17:16:56ZPhysicochemical Investigation of Biosynthesis of a Protein Coating on Glass That Promotes Mammalian Cell Growth Using <i>Lactobacillus rhamnosus</i> GG Bacteria10.3390/coatings111114102079-6412https://doaj.org/article/9d90dff745a7448991a8728393befeb42021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6412/11/11/1410https://doaj.org/toc/2079-6412Glass surfaces, although the first to be used for culturing ex vivo adherent cells, are not the perfect substrates for this purpose. Today, plastics dominate these applications, but in light of the global trend to reduce the use of synthetic polymers, it is reasonable to consider a return to glass vessels with coatings for these purposes. The ideal surface for cell growth is one that simulates the composition and structure of the mainly protein-based intercellular matrix. The work presented here shows a new idea of preparing porous protein coatings on glass using biosynthesis. The process utilizes the colonization of the gold nanoparticle-coated glass surface with <i>Lactobacillus rhamnosus</i> GG bacteria, followed by permeabilization (using ethanol) of their membrane and partial thermal degradation (at 160 °C in vacuum) of the surface-bound protein components of these microorganisms. It results in a development of coating on the glass that promotes mammalian cell growth, which has been preliminary confirmed using Vero cells. Subsequent steps in the formation of coating components were documented by reflectance ultraviolet and visible spectra and infrared spectroscopy. The presence of microorganisms and mammalian cells was confirmed using scanning electron and optical microscopy and crystalline violet staining.Kamil KaminskiKarolina SyrekJoanna GrudzieńMagdalena OblozaMonika AdamczykGrzegorz D. SulkaMDPI AGarticleglass coating<i>Lactobacillus rhamnosus</i> GGcell growthgold nanoparticlesEngineering (General). Civil engineering (General)TA1-2040ENCoatings, Vol 11, Iss 1410, p 1410 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glass coating <i>Lactobacillus rhamnosus</i> GG cell growth gold nanoparticles Engineering (General). Civil engineering (General) TA1-2040
spellingShingle	glass coating <i>Lactobacillus rhamnosus</i> GG cell growth gold nanoparticles Engineering (General). Civil engineering (General) TA1-2040 Kamil Kaminski Karolina Syrek Joanna Grudzień Magdalena Obloza Monika Adamczyk Grzegorz D. Sulka Physicochemical Investigation of Biosynthesis of a Protein Coating on Glass That Promotes Mammalian Cell Growth Using <i>Lactobacillus rhamnosus</i> GG Bacteria
description	Glass surfaces, although the first to be used for culturing ex vivo adherent cells, are not the perfect substrates for this purpose. Today, plastics dominate these applications, but in light of the global trend to reduce the use of synthetic polymers, it is reasonable to consider a return to glass vessels with coatings for these purposes. The ideal surface for cell growth is one that simulates the composition and structure of the mainly protein-based intercellular matrix. The work presented here shows a new idea of preparing porous protein coatings on glass using biosynthesis. The process utilizes the colonization of the gold nanoparticle-coated glass surface with <i>Lactobacillus rhamnosus</i> GG bacteria, followed by permeabilization (using ethanol) of their membrane and partial thermal degradation (at 160 °C in vacuum) of the surface-bound protein components of these microorganisms. It results in a development of coating on the glass that promotes mammalian cell growth, which has been preliminary confirmed using Vero cells. Subsequent steps in the formation of coating components were documented by reflectance ultraviolet and visible spectra and infrared spectroscopy. The presence of microorganisms and mammalian cells was confirmed using scanning electron and optical microscopy and crystalline violet staining.
format	article
author	Kamil Kaminski Karolina Syrek Joanna Grudzień Magdalena Obloza Monika Adamczyk Grzegorz D. Sulka
author_facet	Kamil Kaminski Karolina Syrek Joanna Grudzień Magdalena Obloza Monika Adamczyk Grzegorz D. Sulka
author_sort	Kamil Kaminski
title	Physicochemical Investigation of Biosynthesis of a Protein Coating on Glass That Promotes Mammalian Cell Growth Using <i>Lactobacillus rhamnosus</i> GG Bacteria
title_short	Physicochemical Investigation of Biosynthesis of a Protein Coating on Glass That Promotes Mammalian Cell Growth Using <i>Lactobacillus rhamnosus</i> GG Bacteria
title_full	Physicochemical Investigation of Biosynthesis of a Protein Coating on Glass That Promotes Mammalian Cell Growth Using <i>Lactobacillus rhamnosus</i> GG Bacteria
title_fullStr	Physicochemical Investigation of Biosynthesis of a Protein Coating on Glass That Promotes Mammalian Cell Growth Using <i>Lactobacillus rhamnosus</i> GG Bacteria
title_full_unstemmed	Physicochemical Investigation of Biosynthesis of a Protein Coating on Glass That Promotes Mammalian Cell Growth Using <i>Lactobacillus rhamnosus</i> GG Bacteria
title_sort	physicochemical investigation of biosynthesis of a protein coating on glass that promotes mammalian cell growth using <i>lactobacillus rhamnosus</i> gg bacteria
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9d90dff745a7448991a8728393befeb4
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Physicochemical Investigation of Biosynthesis of a Protein Coating on Glass That Promotes Mammalian Cell Growth Using <i>Lactobacillus rhamnosus</i> GG Bacteria

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