Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA

Abstract The flavescence dorée phytoplasma undergoes a propagative cycle in its insect vectors by first interacting with the insect cell surfaces, primarily in the midgut lumen and subsequently in the salivary glands. Adhesion of flavescence dorée phytoplasma to insect cells is mediated by the adhes...

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Autores principales:	Nathalie Arricau-Bouvery, Sybille Duret, Marie-Pierre Dubrana, Delphine Desqué, Sandrine Eveillard, Lysiane Brocard, Sylvie Malembic-Maher, Xavier Foissac
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d9118444b3f4bd68d7ed80c6a87ed8e
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spelling	oai:doaj.org-article:9d9118444b3f4bd68d7ed80c6a87ed8e2021-12-02T14:42:20ZInteractions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA10.1038/s41598-021-90809-z2045-2322https://doaj.org/article/9d9118444b3f4bd68d7ed80c6a87ed8e2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90809-zhttps://doaj.org/toc/2045-2322Abstract The flavescence dorée phytoplasma undergoes a propagative cycle in its insect vectors by first interacting with the insect cell surfaces, primarily in the midgut lumen and subsequently in the salivary glands. Adhesion of flavescence dorée phytoplasma to insect cells is mediated by the adhesin VmpA. We hypothesize that VmpA may have lectin-like activity, similar to several adhesins of bacteria that invade the insect gut. We first demonstrated that the luminal surface of the midgut and the basal surface of the salivary gland cells of the natural vector Scaphoideus titanus and those of the experimental vector Euscelidius variegatus were differentially glycosylated. Using ELISA, inhibition and competitive adhesion assays, and protein overlay assays in the Euva-6 insect cell line, we showed that the protein VmpA binds insect proteins in a lectin-like manner. In conclusion, the results of this study indicate that N-acetylglucosamine and mannose present on the surfaces of the midgut and salivary glands serve as recognition sites for the phytoplasma adhesin VmpA.Nathalie Arricau-BouverySybille DuretMarie-Pierre DubranaDelphine DesquéSandrine EveillardLysiane BrocardSylvie Malembic-MaherXavier FoissacNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nathalie Arricau-Bouvery Sybille Duret Marie-Pierre Dubrana Delphine Desqué Sandrine Eveillard Lysiane Brocard Sylvie Malembic-Maher Xavier Foissac Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA
description	Abstract The flavescence dorée phytoplasma undergoes a propagative cycle in its insect vectors by first interacting with the insect cell surfaces, primarily in the midgut lumen and subsequently in the salivary glands. Adhesion of flavescence dorée phytoplasma to insect cells is mediated by the adhesin VmpA. We hypothesize that VmpA may have lectin-like activity, similar to several adhesins of bacteria that invade the insect gut. We first demonstrated that the luminal surface of the midgut and the basal surface of the salivary gland cells of the natural vector Scaphoideus titanus and those of the experimental vector Euscelidius variegatus were differentially glycosylated. Using ELISA, inhibition and competitive adhesion assays, and protein overlay assays in the Euva-6 insect cell line, we showed that the protein VmpA binds insect proteins in a lectin-like manner. In conclusion, the results of this study indicate that N-acetylglucosamine and mannose present on the surfaces of the midgut and salivary glands serve as recognition sites for the phytoplasma adhesin VmpA.
format	article
author	Nathalie Arricau-Bouvery Sybille Duret Marie-Pierre Dubrana Delphine Desqué Sandrine Eveillard Lysiane Brocard Sylvie Malembic-Maher Xavier Foissac
author_facet	Nathalie Arricau-Bouvery Sybille Duret Marie-Pierre Dubrana Delphine Desqué Sandrine Eveillard Lysiane Brocard Sylvie Malembic-Maher Xavier Foissac
author_sort	Nathalie Arricau-Bouvery
title	Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA
title_short	Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA
title_full	Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA
title_fullStr	Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA
title_full_unstemmed	Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA
title_sort	interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin vmpa
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9d9118444b3f4bd68d7ed80c6a87ed8e
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Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA

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