In vitro skin culture media influence the viability and inflammatory response of primary macrophages

Abstract The replacement of animal models for investigation of inflammation and wound healing has been advancing by means of in vitro skin equivalents with increasing levels of complexity. However, the current in vitro skin models still have a limited pre-clinical relevance due to their lack of immu...

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Autores principales:	Chiara Griffoni, Berna Neidhart, Ke Yang, Florian Groeber-Becker, Katharina Maniura-Weber, Thomas Dandekar, Heike Walles, Markus Rottmar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d91d63d7a9943f28b8bddb29769ca9c
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spelling	oai:doaj.org-article:9d91d63d7a9943f28b8bddb29769ca9c2021-12-02T13:27:04ZIn vitro skin culture media influence the viability and inflammatory response of primary macrophages10.1038/s41598-021-86486-72045-2322https://doaj.org/article/9d91d63d7a9943f28b8bddb29769ca9c2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86486-7https://doaj.org/toc/2045-2322Abstract The replacement of animal models for investigation of inflammation and wound healing has been advancing by means of in vitro skin equivalents with increasing levels of complexity. However, the current in vitro skin models still have a limited pre-clinical relevance due to their lack of immune cells. So far, few steps have been made towards the incorporation of immune cells into in vitro skin and the requirements for immunocompetent co-cultures remain unexplored. To establish suitable conditions for incorporating macrophages into skin models, we evaluated the effects of different media on primary keratinocytes, fibroblasts and macrophages. Skin maturation was affected by culture in macrophage medium, while macrophages showed reduced viability, altered cell morphology and decreased response to pro- and anti-inflammatory stimuli in skin differentiation media, both in 2D and 3D. The results indicate that immunocompetent skin models have specific, complex requirements for supporting an accurate detection of immune responses, which point at the identification of a suitable culture medium as a crucial pre-requisite for the development of physiologically relevant models.Chiara GriffoniBerna NeidhartKe YangFlorian Groeber-BeckerKatharina Maniura-WeberThomas DandekarHeike WallesMarkus RottmarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chiara Griffoni Berna Neidhart Ke Yang Florian Groeber-Becker Katharina Maniura-Weber Thomas Dandekar Heike Walles Markus Rottmar In vitro skin culture media influence the viability and inflammatory response of primary macrophages
description	Abstract The replacement of animal models for investigation of inflammation and wound healing has been advancing by means of in vitro skin equivalents with increasing levels of complexity. However, the current in vitro skin models still have a limited pre-clinical relevance due to their lack of immune cells. So far, few steps have been made towards the incorporation of immune cells into in vitro skin and the requirements for immunocompetent co-cultures remain unexplored. To establish suitable conditions for incorporating macrophages into skin models, we evaluated the effects of different media on primary keratinocytes, fibroblasts and macrophages. Skin maturation was affected by culture in macrophage medium, while macrophages showed reduced viability, altered cell morphology and decreased response to pro- and anti-inflammatory stimuli in skin differentiation media, both in 2D and 3D. The results indicate that immunocompetent skin models have specific, complex requirements for supporting an accurate detection of immune responses, which point at the identification of a suitable culture medium as a crucial pre-requisite for the development of physiologically relevant models.
format	article
author	Chiara Griffoni Berna Neidhart Ke Yang Florian Groeber-Becker Katharina Maniura-Weber Thomas Dandekar Heike Walles Markus Rottmar
author_facet	Chiara Griffoni Berna Neidhart Ke Yang Florian Groeber-Becker Katharina Maniura-Weber Thomas Dandekar Heike Walles Markus Rottmar
author_sort	Chiara Griffoni
title	In vitro skin culture media influence the viability and inflammatory response of primary macrophages
title_short	In vitro skin culture media influence the viability and inflammatory response of primary macrophages
title_full	In vitro skin culture media influence the viability and inflammatory response of primary macrophages
title_fullStr	In vitro skin culture media influence the viability and inflammatory response of primary macrophages
title_full_unstemmed	In vitro skin culture media influence the viability and inflammatory response of primary macrophages
title_sort	in vitro skin culture media influence the viability and inflammatory response of primary macrophages
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9d91d63d7a9943f28b8bddb29769ca9c
work_keys_str_mv	AT chiaragriffoni invitroskinculturemediainfluencetheviabilityandinflammatoryresponseofprimarymacrophages AT bernaneidhart invitroskinculturemediainfluencetheviabilityandinflammatoryresponseofprimarymacrophages AT keyang invitroskinculturemediainfluencetheviabilityandinflammatoryresponseofprimarymacrophages AT floriangroeberbecker invitroskinculturemediainfluencetheviabilityandinflammatoryresponseofprimarymacrophages AT katharinamaniuraweber invitroskinculturemediainfluencetheviabilityandinflammatoryresponseofprimarymacrophages AT thomasdandekar invitroskinculturemediainfluencetheviabilityandinflammatoryresponseofprimarymacrophages AT heikewalles invitroskinculturemediainfluencetheviabilityandinflammatoryresponseofprimarymacrophages AT markusrottmar invitroskinculturemediainfluencetheviabilityandinflammatoryresponseofprimarymacrophages
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In vitro skin culture media influence the viability and inflammatory response of primary macrophages

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