Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation

Abstract Epidemiological and experimental studies have shown an inverse relationship between infections with certain parasites and a reduced incidence of allergic diseases. We and others have shown that infection with Toxoplasma gondii prevents the development of allergy in mice. To establish whethe...

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Autores principales:	Mirjana Drinić, Angelika Wagner, Priya Sarate, Christian Zwicker, Elke Korb, Gerhard Loupal, Roman Peschke, Anja Joachim, Ursula Wiedermann, Irma Schabussova
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d98377b0ea1474b8fe56b7f5c6479bc
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spelling	oai:doaj.org-article:9d98377b0ea1474b8fe56b7f5c6479bc2021-12-02T11:40:53ZToxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation10.1038/s41598-017-15663-42045-2322https://doaj.org/article/9d98377b0ea1474b8fe56b7f5c6479bc2017-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-15663-4https://doaj.org/toc/2045-2322Abstract Epidemiological and experimental studies have shown an inverse relationship between infections with certain parasites and a reduced incidence of allergic diseases. We and others have shown that infection with Toxoplasma gondii prevents the development of allergy in mice. To establish whether this beneficial effect could be recapitulated by soluble products of this parasite, we tested an extract derived from T. gondii tachyzoites. Immunization of BALB/c mice with tachyzoites lysate antigen (TLA) elicited mixed Th1/Th2 responses. When TLA was applied together with the sensitizing ovalbumin (OVA), the development of allergic airway inflammation was reduced, with decreased airway hyperresponsiveness associated with reduced peribronchial and perivascular cellular infiltration, reduced production of OVA-specific Th2 cytokines in lungs and spleens and reduced levels of serum OVA-specific IgG1 as well as IgE-dependent basophil degranulation. Of note, TLA retained its immunomodulatory properties, inducing high levels of IL-6, TNFα, IL-10 and IL-12p70 in bone marrow-derived dendritic cells after heat-inactivation or proteinase K-treatment for disruption of proteins, but not after sodium metaperiodate-treatment that degrades carbohydrate structures, suggesting that carbohydrates may play a role in immunomodulatory properties of TLA. Here we show that extracts derived from parasites may replicate the benefits of parasitic infection, offering new therapies for immune-mediated disorders.Mirjana DrinićAngelika WagnerPriya SarateChristian ZwickerElke KorbGerhard LoupalRoman PeschkeAnja JoachimUrsula WiedermannIrma SchabussovaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mirjana Drinić Angelika Wagner Priya Sarate Christian Zwicker Elke Korb Gerhard Loupal Roman Peschke Anja Joachim Ursula Wiedermann Irma Schabussova Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation
description	Abstract Epidemiological and experimental studies have shown an inverse relationship between infections with certain parasites and a reduced incidence of allergic diseases. We and others have shown that infection with Toxoplasma gondii prevents the development of allergy in mice. To establish whether this beneficial effect could be recapitulated by soluble products of this parasite, we tested an extract derived from T. gondii tachyzoites. Immunization of BALB/c mice with tachyzoites lysate antigen (TLA) elicited mixed Th1/Th2 responses. When TLA was applied together with the sensitizing ovalbumin (OVA), the development of allergic airway inflammation was reduced, with decreased airway hyperresponsiveness associated with reduced peribronchial and perivascular cellular infiltration, reduced production of OVA-specific Th2 cytokines in lungs and spleens and reduced levels of serum OVA-specific IgG1 as well as IgE-dependent basophil degranulation. Of note, TLA retained its immunomodulatory properties, inducing high levels of IL-6, TNFα, IL-10 and IL-12p70 in bone marrow-derived dendritic cells after heat-inactivation or proteinase K-treatment for disruption of proteins, but not after sodium metaperiodate-treatment that degrades carbohydrate structures, suggesting that carbohydrates may play a role in immunomodulatory properties of TLA. Here we show that extracts derived from parasites may replicate the benefits of parasitic infection, offering new therapies for immune-mediated disorders.
format	article
author	Mirjana Drinić Angelika Wagner Priya Sarate Christian Zwicker Elke Korb Gerhard Loupal Roman Peschke Anja Joachim Ursula Wiedermann Irma Schabussova
author_facet	Mirjana Drinić Angelika Wagner Priya Sarate Christian Zwicker Elke Korb Gerhard Loupal Roman Peschke Anja Joachim Ursula Wiedermann Irma Schabussova
author_sort	Mirjana Drinić
title	Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation
title_short	Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation
title_full	Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation
title_fullStr	Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation
title_full_unstemmed	Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation
title_sort	toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9d98377b0ea1474b8fe56b7f5c6479bc
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Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation

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