Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium

Wei Zhu,1 George Teel,1 Christopher M O’Brien,1 Taisen Zhuang,1 Michael Keidar,1 Lijie Grace Zhang1–3 1Department of Mechanical and Aerospace Engineering, 2Department of Biomedical Engineering, 3Department of Medicine, The George Washington University, Washington, DC, USA Abstr...

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Autores principales:	Zhu W, Teel G, O’Brien CM, Zhuang T, Keidar M, Zhang LG
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Titanium Mesenchymal stem cell Plasma Nanocoating Orthopedics Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9d98ab6d2c94470395719d21b3f46825
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spelling	oai:doaj.org-article:9d98ab6d2c94470395719d21b3f468252021-12-02T07:28:04ZEnhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium1178-2013https://doaj.org/article/9d98ab6d2c94470395719d21b3f468252015-12-01T00:00:00Zhttps://www.dovepress.com/enhanced-human-bone-marrow-mesenchymal-stem-cell-functions-on-cathodic-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wei Zhu,1 George Teel,1 Christopher M O’Brien,1 Taisen Zhuang,1 Michael Keidar,1 Lijie Grace Zhang1–3 1Department of Mechanical and Aerospace Engineering, 2Department of Biomedical Engineering, 3Department of Medicine, The George Washington University, Washington, DC, USA Abstract: Surface modification of titanium for use in orthopedics has been explored for years; however, an ideal method of integrating titanium with native bone is still required to this day. Since human bone cells directly interact with nanostructured extracellular matrices, one of the most promising methods of improving titanium’s osseointegration involves inducing biomimetic nanotopography to enhance cell–implant interaction. In this regard, we explored an approach to functionalize the surface of titanium by depositing a thin film of textured titanium nanoparticles via a cathodic arc discharge plasma. The aim is to improve human bone marrow mesenchymal stem cell (MSC) attachment and differentiation and to reduce deleterious effects of more complex surface modification methods. Surface functionalization was analyzed by scanning electron microscopy, atomic force microscopy, contact angle testing, and specific protein adsorption. Scanning electron microscopy and atomic force microscopy examination demonstrate the deposition of titanium nanoparticles and the surface roughness change after coating. The specific fibronectin adsorption was enhanced on the modified titanium surface that associates with the improved hydrophilicity. MSC adhesion and proliferation were significantly promoted on the nanocoated surface. More importantly, compared to bare titanium, greater production of total protein, deposition of calcium mineral, and synthesis of alkaline phosphatase were observed from MSCs on nanocoated titanium after 21 days. The method described herein presents a promising alternative method for inducing more cell favorable nanosurface for improved orthopedic applications. Keywords: titanium, mesenchymal stem cell, plasma, nanocoating, orthopedicsZhu WTeel GO’Brien CMZhuang TKeidar MZhang LGDove Medical PressarticleTitaniumMesenchymal stem cellPlasmaNanocoatingOrthopedicsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 7385-7396 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Titanium Mesenchymal stem cell Plasma Nanocoating Orthopedics Medicine (General) R5-920
spellingShingle	Titanium Mesenchymal stem cell Plasma Nanocoating Orthopedics Medicine (General) R5-920 Zhu W Teel G O’Brien CM Zhuang T Keidar M Zhang LG Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium
description	Wei Zhu,1 George Teel,1 Christopher M O’Brien,1 Taisen Zhuang,1 Michael Keidar,1 Lijie Grace Zhang1–3 1Department of Mechanical and Aerospace Engineering, 2Department of Biomedical Engineering, 3Department of Medicine, The George Washington University, Washington, DC, USA Abstract: Surface modification of titanium for use in orthopedics has been explored for years; however, an ideal method of integrating titanium with native bone is still required to this day. Since human bone cells directly interact with nanostructured extracellular matrices, one of the most promising methods of improving titanium’s osseointegration involves inducing biomimetic nanotopography to enhance cell–implant interaction. In this regard, we explored an approach to functionalize the surface of titanium by depositing a thin film of textured titanium nanoparticles via a cathodic arc discharge plasma. The aim is to improve human bone marrow mesenchymal stem cell (MSC) attachment and differentiation and to reduce deleterious effects of more complex surface modification methods. Surface functionalization was analyzed by scanning electron microscopy, atomic force microscopy, contact angle testing, and specific protein adsorption. Scanning electron microscopy and atomic force microscopy examination demonstrate the deposition of titanium nanoparticles and the surface roughness change after coating. The specific fibronectin adsorption was enhanced on the modified titanium surface that associates with the improved hydrophilicity. MSC adhesion and proliferation were significantly promoted on the nanocoated surface. More importantly, compared to bare titanium, greater production of total protein, deposition of calcium mineral, and synthesis of alkaline phosphatase were observed from MSCs on nanocoated titanium after 21 days. The method described herein presents a promising alternative method for inducing more cell favorable nanosurface for improved orthopedic applications. Keywords: titanium, mesenchymal stem cell, plasma, nanocoating, orthopedics
format	article
author	Zhu W Teel G O’Brien CM Zhuang T Keidar M Zhang LG
author_facet	Zhu W Teel G O’Brien CM Zhuang T Keidar M Zhang LG
author_sort	Zhu W
title	Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium
title_short	Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium
title_full	Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium
title_fullStr	Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium
title_full_unstemmed	Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium
title_sort	enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/9d98ab6d2c94470395719d21b3f46825
work_keys_str_mv	AT zhuw enhancedhumanbonemarrowmesenchymalstemcellfunctionsoncathodicarcplasmatreatedtitanium AT teelg enhancedhumanbonemarrowmesenchymalstemcellfunctionsoncathodicarcplasmatreatedtitanium AT obriencm enhancedhumanbonemarrowmesenchymalstemcellfunctionsoncathodicarcplasmatreatedtitanium AT zhuangt enhancedhumanbonemarrowmesenchymalstemcellfunctionsoncathodicarcplasmatreatedtitanium AT keidarm enhancedhumanbonemarrowmesenchymalstemcellfunctionsoncathodicarcplasmatreatedtitanium AT zhanglg enhancedhumanbonemarrowmesenchymalstemcellfunctionsoncathodicarcplasmatreatedtitanium
_version_	1718399416739561472

Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium

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