Large-scale identification of extracellular plant miRNAs in mammals implicates their dietary intake.

Extracellular microRNAs (miRNAs) have been proposed to function in cross-kingdom gene regulation. Among these, plant-derived miRNAs of dietary origin have been reported to survive the harsh conditions of the human digestive system, enter the circulatory system, and regulate gene expression and metab...

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Autores principales:	Xi Chen, Lu Liu, Qinjie Chu, Shuo Sun, Yixuan Wu, Zhou Tong, Weijia Fang, Michael P Timko, Longjiang Fan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d9988d077964ad6a32b2c507d30d375
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spelling	oai:doaj.org-article:9d9988d077964ad6a32b2c507d30d3752021-12-02T20:14:03ZLarge-scale identification of extracellular plant miRNAs in mammals implicates their dietary intake.1932-620310.1371/journal.pone.0257878https://doaj.org/article/9d9988d077964ad6a32b2c507d30d3752021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257878https://doaj.org/toc/1932-6203Extracellular microRNAs (miRNAs) have been proposed to function in cross-kingdom gene regulation. Among these, plant-derived miRNAs of dietary origin have been reported to survive the harsh conditions of the human digestive system, enter the circulatory system, and regulate gene expression and metabolic function. However, definitive evidence supporting the presence of plant-derived miRNAs of dietary origin in mammals has been difficult to obtain due to limited sample sizes. We have developed a bioinformatics pipeline (ePmiRNA_finder) that provides strident miRNA classification and applied it to analyze 421 small RNA sequencing data sets from 10 types of human body fluids and tissues and comparative samples from carnivores and herbivores. A total of 35 miRNAs were identified that map to plants typically found in the human diet and these miRNAs were found in at least one human blood sample and their abundance was significantly different when compared to samples from human microbiome or cow. The plant-derived miRNA profiles were body fluid/tissue-specific and highly abundant in the brain and the breast milk samples, indicating selective absorption and/or the ability to be transported across tissue/organ barriers. Our data provide conclusive evidence for the presence of plant-derived miRNAs as a consequence of dietary intake and their cross-kingdom regulatory function within human circulating system.Xi ChenLu LiuQinjie ChuShuo SunYixuan WuZhou TongWeijia FangMichael P TimkoLongjiang FanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257878 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xi Chen Lu Liu Qinjie Chu Shuo Sun Yixuan Wu Zhou Tong Weijia Fang Michael P Timko Longjiang Fan Large-scale identification of extracellular plant miRNAs in mammals implicates their dietary intake.
description	Extracellular microRNAs (miRNAs) have been proposed to function in cross-kingdom gene regulation. Among these, plant-derived miRNAs of dietary origin have been reported to survive the harsh conditions of the human digestive system, enter the circulatory system, and regulate gene expression and metabolic function. However, definitive evidence supporting the presence of plant-derived miRNAs of dietary origin in mammals has been difficult to obtain due to limited sample sizes. We have developed a bioinformatics pipeline (ePmiRNA_finder) that provides strident miRNA classification and applied it to analyze 421 small RNA sequencing data sets from 10 types of human body fluids and tissues and comparative samples from carnivores and herbivores. A total of 35 miRNAs were identified that map to plants typically found in the human diet and these miRNAs were found in at least one human blood sample and their abundance was significantly different when compared to samples from human microbiome or cow. The plant-derived miRNA profiles were body fluid/tissue-specific and highly abundant in the brain and the breast milk samples, indicating selective absorption and/or the ability to be transported across tissue/organ barriers. Our data provide conclusive evidence for the presence of plant-derived miRNAs as a consequence of dietary intake and their cross-kingdom regulatory function within human circulating system.
format	article
author	Xi Chen Lu Liu Qinjie Chu Shuo Sun Yixuan Wu Zhou Tong Weijia Fang Michael P Timko Longjiang Fan
author_facet	Xi Chen Lu Liu Qinjie Chu Shuo Sun Yixuan Wu Zhou Tong Weijia Fang Michael P Timko Longjiang Fan
author_sort	Xi Chen
title	Large-scale identification of extracellular plant miRNAs in mammals implicates their dietary intake.
title_short	Large-scale identification of extracellular plant miRNAs in mammals implicates their dietary intake.
title_full	Large-scale identification of extracellular plant miRNAs in mammals implicates their dietary intake.
title_fullStr	Large-scale identification of extracellular plant miRNAs in mammals implicates their dietary intake.
title_full_unstemmed	Large-scale identification of extracellular plant miRNAs in mammals implicates their dietary intake.
title_sort	large-scale identification of extracellular plant mirnas in mammals implicates their dietary intake.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/9d9988d077964ad6a32b2c507d30d375
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Large-scale identification of extracellular plant miRNAs in mammals implicates their dietary intake.

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