Growth differentiation factor 15 promotes blood vessel growth by stimulating cell cycle progression in repair of critical-sized calvarial defect

Abstract Repair of large bone defects remains a challenge for surgeons, tissue engineering represents a promising approach. However, the use of this technique is limited by delayed vascularization in central regions of the scaffold. Growth differentiation factor 15(GDF15) has recently been reported...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shaoyi Wang, Mengyu Li, Wenjie Zhang, Hongfei Hua, Ningtao Wang, Jun Zhao, Jing Ge, Xinquan Jiang, Zhiyuan Zhang, Dongxia Ye, Chi Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d99abbf26ed406ba639ac350c4555e3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9d99abbf26ed406ba639ac350c4555e3
record_format	dspace
spelling	oai:doaj.org-article:9d99abbf26ed406ba639ac350c4555e32021-12-02T16:06:21ZGrowth differentiation factor 15 promotes blood vessel growth by stimulating cell cycle progression in repair of critical-sized calvarial defect10.1038/s41598-017-09210-42045-2322https://doaj.org/article/9d99abbf26ed406ba639ac350c4555e32017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09210-4https://doaj.org/toc/2045-2322Abstract Repair of large bone defects remains a challenge for surgeons, tissue engineering represents a promising approach. However, the use of this technique is limited by delayed vascularization in central regions of the scaffold. Growth differentiation factor 15(GDF15) has recently been reported to be a potential angiogenic cytokine and has an ability to promote the proliferation of human umbilical vein endothelial cells(HUVECs). Whether it can be applied for promoting vascularized bone regeneration is still unknown. In this study, we demonstrated that GDF15 augmented the expression of cyclins D1 and E, induced Rb phosphorylation and E2F-1 nuclear translocation, as well as increased HUVECs proliferation. Furthermore, we also observed that GDF15 promoted the formation of functional vessels at an artificially-induced angiogenic site, and remarkably improved the healing in the repair of critical-sized calvarial defects. Our results confirm the essential role of GDF15 in angiogenesis and suggest its potential beneficial use in regenerative medicine.Shaoyi WangMengyu LiWenjie ZhangHongfei HuaNingtao WangJun ZhaoJing GeXinquan JiangZhiyuan ZhangDongxia YeChi YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shaoyi Wang Mengyu Li Wenjie Zhang Hongfei Hua Ningtao Wang Jun Zhao Jing Ge Xinquan Jiang Zhiyuan Zhang Dongxia Ye Chi Yang Growth differentiation factor 15 promotes blood vessel growth by stimulating cell cycle progression in repair of critical-sized calvarial defect
description	Abstract Repair of large bone defects remains a challenge for surgeons, tissue engineering represents a promising approach. However, the use of this technique is limited by delayed vascularization in central regions of the scaffold. Growth differentiation factor 15(GDF15) has recently been reported to be a potential angiogenic cytokine and has an ability to promote the proliferation of human umbilical vein endothelial cells(HUVECs). Whether it can be applied for promoting vascularized bone regeneration is still unknown. In this study, we demonstrated that GDF15 augmented the expression of cyclins D1 and E, induced Rb phosphorylation and E2F-1 nuclear translocation, as well as increased HUVECs proliferation. Furthermore, we also observed that GDF15 promoted the formation of functional vessels at an artificially-induced angiogenic site, and remarkably improved the healing in the repair of critical-sized calvarial defects. Our results confirm the essential role of GDF15 in angiogenesis and suggest its potential beneficial use in regenerative medicine.
format	article
author	Shaoyi Wang Mengyu Li Wenjie Zhang Hongfei Hua Ningtao Wang Jun Zhao Jing Ge Xinquan Jiang Zhiyuan Zhang Dongxia Ye Chi Yang
author_facet	Shaoyi Wang Mengyu Li Wenjie Zhang Hongfei Hua Ningtao Wang Jun Zhao Jing Ge Xinquan Jiang Zhiyuan Zhang Dongxia Ye Chi Yang
author_sort	Shaoyi Wang
title	Growth differentiation factor 15 promotes blood vessel growth by stimulating cell cycle progression in repair of critical-sized calvarial defect
title_short	Growth differentiation factor 15 promotes blood vessel growth by stimulating cell cycle progression in repair of critical-sized calvarial defect
title_full	Growth differentiation factor 15 promotes blood vessel growth by stimulating cell cycle progression in repair of critical-sized calvarial defect
title_fullStr	Growth differentiation factor 15 promotes blood vessel growth by stimulating cell cycle progression in repair of critical-sized calvarial defect
title_full_unstemmed	Growth differentiation factor 15 promotes blood vessel growth by stimulating cell cycle progression in repair of critical-sized calvarial defect
title_sort	growth differentiation factor 15 promotes blood vessel growth by stimulating cell cycle progression in repair of critical-sized calvarial defect
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9d99abbf26ed406ba639ac350c4555e3
work_keys_str_mv	AT shaoyiwang growthdifferentiationfactor15promotesbloodvesselgrowthbystimulatingcellcycleprogressioninrepairofcriticalsizedcalvarialdefect AT mengyuli growthdifferentiationfactor15promotesbloodvesselgrowthbystimulatingcellcycleprogressioninrepairofcriticalsizedcalvarialdefect AT wenjiezhang growthdifferentiationfactor15promotesbloodvesselgrowthbystimulatingcellcycleprogressioninrepairofcriticalsizedcalvarialdefect AT hongfeihua growthdifferentiationfactor15promotesbloodvesselgrowthbystimulatingcellcycleprogressioninrepairofcriticalsizedcalvarialdefect AT ningtaowang growthdifferentiationfactor15promotesbloodvesselgrowthbystimulatingcellcycleprogressioninrepairofcriticalsizedcalvarialdefect AT junzhao growthdifferentiationfactor15promotesbloodvesselgrowthbystimulatingcellcycleprogressioninrepairofcriticalsizedcalvarialdefect AT jingge growthdifferentiationfactor15promotesbloodvesselgrowthbystimulatingcellcycleprogressioninrepairofcriticalsizedcalvarialdefect AT xinquanjiang growthdifferentiationfactor15promotesbloodvesselgrowthbystimulatingcellcycleprogressioninrepairofcriticalsizedcalvarialdefect AT zhiyuanzhang growthdifferentiationfactor15promotesbloodvesselgrowthbystimulatingcellcycleprogressioninrepairofcriticalsizedcalvarialdefect AT dongxiaye growthdifferentiationfactor15promotesbloodvesselgrowthbystimulatingcellcycleprogressioninrepairofcriticalsizedcalvarialdefect AT chiyang growthdifferentiationfactor15promotesbloodvesselgrowthbystimulatingcellcycleprogressioninrepairofcriticalsizedcalvarialdefect
_version_	1718385041557422080

Growth differentiation factor 15 promotes blood vessel growth by stimulating cell cycle progression in repair of critical-sized calvarial defect

Ejemplares similares