Optimization of hormone combinations for root growth and bud germination in Chinese fir (Cunninghamia lanceolata) clone leaf cuttings

Abstract In order to ascertain the optimal hormone combination for Chinese fir (Cunninghamia lanceolata) leaf cuttings, an L16(4)4 orthogonal test of 6-benzylaminopurine (6-BA), 1-naphthaleneacetic acid (NAA), and indole-3-butyric acid (IBA) (0, 10, 30, or 50 mg · L−1 of each exogenous hormone) imme...

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Autores principales:	Shubin Li, Peng Huang, Guochang Ding, Lili Zhou, Piao Tang, Min Sun, Yingying Zheng, Sizu Lin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d9da05f0b0b4b67ad36743a5805b5ef
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spelling	oai:doaj.org-article:9d9da05f0b0b4b67ad36743a5805b5ef2021-12-02T16:06:57ZOptimization of hormone combinations for root growth and bud germination in Chinese fir (Cunninghamia lanceolata) clone leaf cuttings10.1038/s41598-017-05295-z2045-2322https://doaj.org/article/9d9da05f0b0b4b67ad36743a5805b5ef2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05295-zhttps://doaj.org/toc/2045-2322Abstract In order to ascertain the optimal hormone combination for Chinese fir (Cunninghamia lanceolata) leaf cuttings, an L16(4)4 orthogonal test of 6-benzylaminopurine (6-BA), 1-naphthaleneacetic acid (NAA), and indole-3-butyric acid (IBA) (0, 10, 30, or 50 mg · L−1 of each exogenous hormone) immersion for 5, 10, 15, or 20 min was conducted. Callus initiation rate and rooting promotion rate were mainly affected by treatment time, root length increase by 6-BA concentration, and bud germination rate and plantlet formation rate by NAA concentration. The expected optimal combination for callus initiation rate was 50 mg · L−1 6-BA + 0 mg · L−1 NAA + 30 mg · L−1 IBA + 10 min; for rooting promotion rate, it was 0–10 mg · L−1 6-BA + 10 mg · L−1 NAA + 30 mg · L−1 IBA + 20 min; for bud germination rate, it was 50 mg · L−1 6-BA + 0–10 mg · L−1 NAA + 0–30 mg · L−1 IBA + 20 min; and for seedling formation rate for No. 4, it was 10 mg · L−1 6-BA + 10 mg · L−1 NAA + 0 mg · L−1 IBA + 20 min. Light microscopy image analysis revealed that a cluster of primordial cells was produced 40 days after cutting, and mastoid cells developed into peninsula cells in calli that were cultured for 50 days.Shubin LiPeng HuangGuochang DingLili ZhouPiao TangMin SunYingying ZhengSizu LinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shubin Li Peng Huang Guochang Ding Lili Zhou Piao Tang Min Sun Yingying Zheng Sizu Lin Optimization of hormone combinations for root growth and bud germination in Chinese fir (Cunninghamia lanceolata) clone leaf cuttings
description	Abstract In order to ascertain the optimal hormone combination for Chinese fir (Cunninghamia lanceolata) leaf cuttings, an L16(4)4 orthogonal test of 6-benzylaminopurine (6-BA), 1-naphthaleneacetic acid (NAA), and indole-3-butyric acid (IBA) (0, 10, 30, or 50 mg · L−1 of each exogenous hormone) immersion for 5, 10, 15, or 20 min was conducted. Callus initiation rate and rooting promotion rate were mainly affected by treatment time, root length increase by 6-BA concentration, and bud germination rate and plantlet formation rate by NAA concentration. The expected optimal combination for callus initiation rate was 50 mg · L−1 6-BA + 0 mg · L−1 NAA + 30 mg · L−1 IBA + 10 min; for rooting promotion rate, it was 0–10 mg · L−1 6-BA + 10 mg · L−1 NAA + 30 mg · L−1 IBA + 20 min; for bud germination rate, it was 50 mg · L−1 6-BA + 0–10 mg · L−1 NAA + 0–30 mg · L−1 IBA + 20 min; and for seedling formation rate for No. 4, it was 10 mg · L−1 6-BA + 10 mg · L−1 NAA + 0 mg · L−1 IBA + 20 min. Light microscopy image analysis revealed that a cluster of primordial cells was produced 40 days after cutting, and mastoid cells developed into peninsula cells in calli that were cultured for 50 days.
format	article
author	Shubin Li Peng Huang Guochang Ding Lili Zhou Piao Tang Min Sun Yingying Zheng Sizu Lin
author_facet	Shubin Li Peng Huang Guochang Ding Lili Zhou Piao Tang Min Sun Yingying Zheng Sizu Lin
author_sort	Shubin Li
title	Optimization of hormone combinations for root growth and bud germination in Chinese fir (Cunninghamia lanceolata) clone leaf cuttings
title_short	Optimization of hormone combinations for root growth and bud germination in Chinese fir (Cunninghamia lanceolata) clone leaf cuttings
title_full	Optimization of hormone combinations for root growth and bud germination in Chinese fir (Cunninghamia lanceolata) clone leaf cuttings
title_fullStr	Optimization of hormone combinations for root growth and bud germination in Chinese fir (Cunninghamia lanceolata) clone leaf cuttings
title_full_unstemmed	Optimization of hormone combinations for root growth and bud germination in Chinese fir (Cunninghamia lanceolata) clone leaf cuttings
title_sort	optimization of hormone combinations for root growth and bud germination in chinese fir (cunninghamia lanceolata) clone leaf cuttings
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9d9da05f0b0b4b67ad36743a5805b5ef
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Optimization of hormone combinations for root growth and bud germination in Chinese fir (Cunninghamia lanceolata) clone leaf cuttings

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