Antibody responses to Zika virus proteins in pregnant and non-pregnant macaques.

The specificity of the antibody response against Zika virus (ZIKV) is not well-characterized. This is due, in part, to the antigenic similarity between ZIKV and closely related dengue virus (DENV) serotypes. Since these and other similar viruses co-circulate, are spread by the same mosquito species,...

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Autores principales:	Anna S Heffron, Emma L Mohr, David Baker, Amelia K Haj, Connor R Buechler, Adam Bailey, Dawn M Dudley, Christina M Newman, Mariel S Mohns, Michelle Koenig, Meghan E Breitbach, Mustafa Rasheed, Laurel M Stewart, Jens Eickhoff, Richard S Pinapati, Erica Beckman, Hanying Li, Jigar Patel, John C Tan, David H O'Connor
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2018
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/9da17d78e2994815a220f4d6d6ca4b9a
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spelling	oai:doaj.org-article:9da17d78e2994815a220f4d6d6ca4b9a2021-12-02T20:23:28ZAntibody responses to Zika virus proteins in pregnant and non-pregnant macaques.1935-27271935-273510.1371/journal.pntd.0006903https://doaj.org/article/9da17d78e2994815a220f4d6d6ca4b9a2018-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0006903https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735The specificity of the antibody response against Zika virus (ZIKV) is not well-characterized. This is due, in part, to the antigenic similarity between ZIKV and closely related dengue virus (DENV) serotypes. Since these and other similar viruses co-circulate, are spread by the same mosquito species, and can cause similar acute clinical syndromes, it is difficult to disentangle ZIKV-specific antibody responses from responses to closely-related arboviruses in humans. Here we use high-density peptide microarrays to profile anti-ZIKV antibody reactivity in pregnant and non-pregnant macaque monkeys with known exposure histories and compare these results to reactivity following DENV infection. We also compare cross-reactive binding of ZIKV-immune sera to the full proteomes of 28 arboviruses. We independently confirm a purported ZIKV-specific IgG antibody response targeting ZIKV nonstructural protein 2B (NS2B) that was recently reported in ZIKV-infected people and we show that antibody reactivity in pregnant animals can be detected as late as 127 days post-infection (dpi). However, we also show that these responses wane over time, sometimes rapidly, and in one case the response was elicited following DENV infection in a previously ZIKV-exposed animal. These results suggest epidemiologic studies assessing seroprevalence of ZIKV immunity using linear epitope-based strategies will remain challenging to interpret due to susceptibility to false positive results. However, the method used here demonstrates the potential for rapid profiling of proteome-wide antibody responses to a myriad of neglected diseases simultaneously and may be especially useful for distinguishing antibody reactivity among closely related pathogens.Anna S HeffronEmma L MohrDavid BakerAmelia K HajConnor R BuechlerAdam BaileyDawn M DudleyChristina M NewmanMariel S MohnsMichelle KoenigMeghan E BreitbachMustafa RasheedLaurel M StewartJens EickhoffRichard S PinapatiErica BeckmanHanying LiJigar PatelJohn C TanDavid H O'ConnorPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 12, Iss 11, p e0006903 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Anna S Heffron Emma L Mohr David Baker Amelia K Haj Connor R Buechler Adam Bailey Dawn M Dudley Christina M Newman Mariel S Mohns Michelle Koenig Meghan E Breitbach Mustafa Rasheed Laurel M Stewart Jens Eickhoff Richard S Pinapati Erica Beckman Hanying Li Jigar Patel John C Tan David H O'Connor Antibody responses to Zika virus proteins in pregnant and non-pregnant macaques.
description	The specificity of the antibody response against Zika virus (ZIKV) is not well-characterized. This is due, in part, to the antigenic similarity between ZIKV and closely related dengue virus (DENV) serotypes. Since these and other similar viruses co-circulate, are spread by the same mosquito species, and can cause similar acute clinical syndromes, it is difficult to disentangle ZIKV-specific antibody responses from responses to closely-related arboviruses in humans. Here we use high-density peptide microarrays to profile anti-ZIKV antibody reactivity in pregnant and non-pregnant macaque monkeys with known exposure histories and compare these results to reactivity following DENV infection. We also compare cross-reactive binding of ZIKV-immune sera to the full proteomes of 28 arboviruses. We independently confirm a purported ZIKV-specific IgG antibody response targeting ZIKV nonstructural protein 2B (NS2B) that was recently reported in ZIKV-infected people and we show that antibody reactivity in pregnant animals can be detected as late as 127 days post-infection (dpi). However, we also show that these responses wane over time, sometimes rapidly, and in one case the response was elicited following DENV infection in a previously ZIKV-exposed animal. These results suggest epidemiologic studies assessing seroprevalence of ZIKV immunity using linear epitope-based strategies will remain challenging to interpret due to susceptibility to false positive results. However, the method used here demonstrates the potential for rapid profiling of proteome-wide antibody responses to a myriad of neglected diseases simultaneously and may be especially useful for distinguishing antibody reactivity among closely related pathogens.
format	article
author	Anna S Heffron Emma L Mohr David Baker Amelia K Haj Connor R Buechler Adam Bailey Dawn M Dudley Christina M Newman Mariel S Mohns Michelle Koenig Meghan E Breitbach Mustafa Rasheed Laurel M Stewart Jens Eickhoff Richard S Pinapati Erica Beckman Hanying Li Jigar Patel John C Tan David H O'Connor
author_facet	Anna S Heffron Emma L Mohr David Baker Amelia K Haj Connor R Buechler Adam Bailey Dawn M Dudley Christina M Newman Mariel S Mohns Michelle Koenig Meghan E Breitbach Mustafa Rasheed Laurel M Stewart Jens Eickhoff Richard S Pinapati Erica Beckman Hanying Li Jigar Patel John C Tan David H O'Connor
author_sort	Anna S Heffron
title	Antibody responses to Zika virus proteins in pregnant and non-pregnant macaques.
title_short	Antibody responses to Zika virus proteins in pregnant and non-pregnant macaques.
title_full	Antibody responses to Zika virus proteins in pregnant and non-pregnant macaques.
title_fullStr	Antibody responses to Zika virus proteins in pregnant and non-pregnant macaques.
title_full_unstemmed	Antibody responses to Zika virus proteins in pregnant and non-pregnant macaques.
title_sort	antibody responses to zika virus proteins in pregnant and non-pregnant macaques.
publisher	Public Library of Science (PLoS)
publishDate	2018
url	https://doaj.org/article/9da17d78e2994815a220f4d6d6ca4b9a
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Antibody responses to Zika virus proteins in pregnant and non-pregnant macaques.

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