Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level

Existing methods for protein polymer engineering suffer from low efficiency especially for synthesis large size polyproteins. Here, Deng et al. construct homo-polymer and co-polymer up to decamer by stepwise ligation and cleavage validated by atomic force microscopy-based single-molecule force spect...

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Autores principales:	Yibing Deng, Tao Wu, Mengdi Wang, Shengchao Shi, Guodong Yuan, Xi Li, Hanchung Chong, Bin Wu, Peng Zheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/9da1a8ddd527406cab7cc9175b041dfa
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spelling	oai:doaj.org-article:9da1a8ddd527406cab7cc9175b041dfa2021-12-02T16:57:44ZEnzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level10.1038/s41467-019-10696-x2041-1723https://doaj.org/article/9da1a8ddd527406cab7cc9175b041dfa2019-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10696-xhttps://doaj.org/toc/2041-1723Existing methods for protein polymer engineering suffer from low efficiency especially for synthesis large size polyproteins. Here, Deng et al. construct homo-polymer and co-polymer up to decamer by stepwise ligation and cleavage validated by atomic force microscopy-based single-molecule force spectroscopy.Yibing DengTao WuMengdi WangShengchao ShiGuodong YuanXi LiHanchung ChongBin WuPeng ZhengNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yibing Deng Tao Wu Mengdi Wang Shengchao Shi Guodong Yuan Xi Li Hanchung Chong Bin Wu Peng Zheng Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level
description	Existing methods for protein polymer engineering suffer from low efficiency especially for synthesis large size polyproteins. Here, Deng et al. construct homo-polymer and co-polymer up to decamer by stepwise ligation and cleavage validated by atomic force microscopy-based single-molecule force spectroscopy.
format	article
author	Yibing Deng Tao Wu Mengdi Wang Shengchao Shi Guodong Yuan Xi Li Hanchung Chong Bin Wu Peng Zheng
author_facet	Yibing Deng Tao Wu Mengdi Wang Shengchao Shi Guodong Yuan Xi Li Hanchung Chong Bin Wu Peng Zheng
author_sort	Yibing Deng
title	Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level
title_short	Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level
title_full	Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level
title_fullStr	Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level
title_full_unstemmed	Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level
title_sort	enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/9da1a8ddd527406cab7cc9175b041dfa
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Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level

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