Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level

Existing methods for protein polymer engineering suffer from low efficiency especially for synthesis large size polyproteins. Here, Deng et al. construct homo-polymer and co-polymer up to decamer by stepwise ligation and cleavage validated by atomic force microscopy-based single-molecule force spect...

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Autores principales: Yibing Deng, Tao Wu, Mengdi Wang, Shengchao Shi, Guodong Yuan, Xi Li, Hanchung Chong, Bin Wu, Peng Zheng
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/9da1a8ddd527406cab7cc9175b041dfa
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