Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates

Abstract Plasmodium falciparum proteins involved in erythrocyte invasion are main targets of acquired immunity and important vaccine candidates. We hypothesized that anti-parasite immunity acquired upon exposure would limit invasion-related gene (IRG) expression and affect the clinical impact of the...

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Autores principales:	Aida Valmaseda, Quique Bassat, Pedro Aide, Pau Cisteró, Alfons Jiménez, Aina Casellas, Sonia Machevo, Ruth Aguilar, Betuel Sigaúque, Virander S. Chauhan, Christine Langer, James Beeson, Chetan Chitnis, Pedro L. Alonso, Deepak Gaur, Alfredo Mayor
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9da294e70b2248cc902b62b89b355640
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spelling	oai:doaj.org-article:9da294e70b2248cc902b62b89b3556402021-12-02T16:06:12ZHost age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates10.1038/s41598-017-05025-52045-2322https://doaj.org/article/9da294e70b2248cc902b62b89b3556402017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05025-5https://doaj.org/toc/2045-2322Abstract Plasmodium falciparum proteins involved in erythrocyte invasion are main targets of acquired immunity and important vaccine candidates. We hypothesized that anti-parasite immunity acquired upon exposure would limit invasion-related gene (IRG) expression and affect the clinical impact of the infection. 11 IRG transcript levels were measured in P. falciparum isolates by RT-PCR, and IgG/IgM against invasion ligands by Luminex®, in 50 Mozambican adults, 25 children with severe malaria (SM) and 25 with uncomplicated malaria (UM). IRG expression differences among groups and associations between IRG expression and clinical/immunologic parameters were assessed. IRG expression diversity was higher in parasites infecting children than adults (p = 0.022). eba140 and ptramp expression decreased with age (p = 0.003 and 0.007, respectively) whereas p41 expression increased (p = 0.022). pfrh5 reduction in expression was abrupt early in life. Parasite density decreased with increasing pfrh5 expression (p < 0.001) and, only in children, parasite density increased with p41 expression (p = 0.007), and decreased with eba175 (p = 0.013). Antibody responses and IRG expression were not associated. In conclusion, IRG expression is associated with age and parasite density, but not with specific antibody responses in the acute phase of infection. Our results confirm the importance of multi-antigen vaccines development to avoid parasite immune escape when tested in malaria-exposed individuals.Aida ValmasedaQuique BassatPedro AidePau CisteróAlfons JiménezAina CasellasSonia MachevoRuth AguilarBetuel SigaúqueVirander S. ChauhanChristine LangerJames BeesonChetan ChitnisPedro L. AlonsoDeepak GaurAlfredo MayorNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aida Valmaseda Quique Bassat Pedro Aide Pau Cisteró Alfons Jiménez Aina Casellas Sonia Machevo Ruth Aguilar Betuel Sigaúque Virander S. Chauhan Christine Langer James Beeson Chetan Chitnis Pedro L. Alonso Deepak Gaur Alfredo Mayor Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates
description	Abstract Plasmodium falciparum proteins involved in erythrocyte invasion are main targets of acquired immunity and important vaccine candidates. We hypothesized that anti-parasite immunity acquired upon exposure would limit invasion-related gene (IRG) expression and affect the clinical impact of the infection. 11 IRG transcript levels were measured in P. falciparum isolates by RT-PCR, and IgG/IgM against invasion ligands by Luminex®, in 50 Mozambican adults, 25 children with severe malaria (SM) and 25 with uncomplicated malaria (UM). IRG expression differences among groups and associations between IRG expression and clinical/immunologic parameters were assessed. IRG expression diversity was higher in parasites infecting children than adults (p = 0.022). eba140 and ptramp expression decreased with age (p = 0.003 and 0.007, respectively) whereas p41 expression increased (p = 0.022). pfrh5 reduction in expression was abrupt early in life. Parasite density decreased with increasing pfrh5 expression (p < 0.001) and, only in children, parasite density increased with p41 expression (p = 0.007), and decreased with eba175 (p = 0.013). Antibody responses and IRG expression were not associated. In conclusion, IRG expression is associated with age and parasite density, but not with specific antibody responses in the acute phase of infection. Our results confirm the importance of multi-antigen vaccines development to avoid parasite immune escape when tested in malaria-exposed individuals.
format	article
author	Aida Valmaseda Quique Bassat Pedro Aide Pau Cisteró Alfons Jiménez Aina Casellas Sonia Machevo Ruth Aguilar Betuel Sigaúque Virander S. Chauhan Christine Langer James Beeson Chetan Chitnis Pedro L. Alonso Deepak Gaur Alfredo Mayor
author_facet	Aida Valmaseda Quique Bassat Pedro Aide Pau Cisteró Alfons Jiménez Aina Casellas Sonia Machevo Ruth Aguilar Betuel Sigaúque Virander S. Chauhan Christine Langer James Beeson Chetan Chitnis Pedro L. Alonso Deepak Gaur Alfredo Mayor
author_sort	Aida Valmaseda
title	Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates
title_short	Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates
title_full	Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates
title_fullStr	Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates
title_full_unstemmed	Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates
title_sort	host age and expression of genes involved in red blood cell invasion in plasmodium falciparum field isolates
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9da294e70b2248cc902b62b89b355640
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Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates

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