Admixed phylogenetic distribution of drug resistant Mycobacterium tuberculosis in Saudi Arabia.

<h4>Background</h4>The phylogeographical structure of Mycobacterium tuberculosis is generally bimodal in low tuberculosis (TB) incidence countries, where genetic lineages of the isolates generally differ with little strain clustering between autochthonous and foreign-born TB patients. Ho...

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Autores principales:	Bright Varghese, Philip Supply, Caroline Allix-Béguec, Mohammed Shoukri, Ruba Al-Omari, Mais Herbawi, Sahal Al-Hajoj
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9da2cb1bec7f42d1a56fb8389e0e137f
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spelling	oai:doaj.org-article:9da2cb1bec7f42d1a56fb8389e0e137f2021-11-18T07:58:58ZAdmixed phylogenetic distribution of drug resistant Mycobacterium tuberculosis in Saudi Arabia.1932-620310.1371/journal.pone.0055598https://doaj.org/article/9da2cb1bec7f42d1a56fb8389e0e137f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23383340/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The phylogeographical structure of Mycobacterium tuberculosis is generally bimodal in low tuberculosis (TB) incidence countries, where genetic lineages of the isolates generally differ with little strain clustering between autochthonous and foreign-born TB patients. However, less is known on this structure in Saudi Arabia-the most important hub of human migration as it hosts a total population of expatriates and pilgrims from all over the world which is equal to that of its citizens.<h4>Methodology</h4>We explored the mycobacterial phylogenetic structure and strain molecular clustering in Saudi Arabia by genotyping 322 drug-resistant clinical isolates collected over a 12-month period in a national drug surveillance survey, using 24 locus-based MIRU-VNTR typing and spoligotyping.<h4>Principal findings</h4>In contrast to the cosmopolitan population of the country, almost all the known phylogeographic lineages of M. tuberculosis complex (with noticeable exception of Mycobacterium africanum/West-African 1 and 2) were detected, with Delhi/CAS (21.1%), EAI (11.2%), Beijing (11.2%) and main branches of the Euro-American super-lineage such as Ghana (14.9%), Haarlem (10.6%) and Cameroon (7.8%) being represented. Statistically significant associations of strain lineages were observed with poly-drug resistance and multi drug resistance especially among previously treated cases (p value of < = 0.001 for both types of resistance), with relative over-representation of Beijing strains in the latter category. However, there was no significant difference among Saudi and non-Saudi TB patients regarding distribution of phylogenetic lineages (p = 0.311). Moreover, 59.5% (22/37) of the strain molecular clusters were shared between the Saudi born and immigrant TB patients.<h4>Conclusions</h4>Specific distribution of M. tuberculosis phylogeographic lineages is not observed between the autochthonous and foreign-born populations. These observations might reflect both socially favored ongoing TB transmission between the two population groups, and historically deep-rooted, prolonged contacts and trade relations of the peninsula with other world regions. More vigorous surveillance and strict adherence to tuberculosis control policies are urgently needed in the country.Bright VarghesePhilip SupplyCaroline Allix-BéguecMohammed ShoukriRuba Al-OmariMais HerbawiSahal Al-HajojPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e55598 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bright Varghese Philip Supply Caroline Allix-Béguec Mohammed Shoukri Ruba Al-Omari Mais Herbawi Sahal Al-Hajoj Admixed phylogenetic distribution of drug resistant Mycobacterium tuberculosis in Saudi Arabia.
description	<h4>Background</h4>The phylogeographical structure of Mycobacterium tuberculosis is generally bimodal in low tuberculosis (TB) incidence countries, where genetic lineages of the isolates generally differ with little strain clustering between autochthonous and foreign-born TB patients. However, less is known on this structure in Saudi Arabia-the most important hub of human migration as it hosts a total population of expatriates and pilgrims from all over the world which is equal to that of its citizens.<h4>Methodology</h4>We explored the mycobacterial phylogenetic structure and strain molecular clustering in Saudi Arabia by genotyping 322 drug-resistant clinical isolates collected over a 12-month period in a national drug surveillance survey, using 24 locus-based MIRU-VNTR typing and spoligotyping.<h4>Principal findings</h4>In contrast to the cosmopolitan population of the country, almost all the known phylogeographic lineages of M. tuberculosis complex (with noticeable exception of Mycobacterium africanum/West-African 1 and 2) were detected, with Delhi/CAS (21.1%), EAI (11.2%), Beijing (11.2%) and main branches of the Euro-American super-lineage such as Ghana (14.9%), Haarlem (10.6%) and Cameroon (7.8%) being represented. Statistically significant associations of strain lineages were observed with poly-drug resistance and multi drug resistance especially among previously treated cases (p value of < = 0.001 for both types of resistance), with relative over-representation of Beijing strains in the latter category. However, there was no significant difference among Saudi and non-Saudi TB patients regarding distribution of phylogenetic lineages (p = 0.311). Moreover, 59.5% (22/37) of the strain molecular clusters were shared between the Saudi born and immigrant TB patients.<h4>Conclusions</h4>Specific distribution of M. tuberculosis phylogeographic lineages is not observed between the autochthonous and foreign-born populations. These observations might reflect both socially favored ongoing TB transmission between the two population groups, and historically deep-rooted, prolonged contacts and trade relations of the peninsula with other world regions. More vigorous surveillance and strict adherence to tuberculosis control policies are urgently needed in the country.
format	article
author	Bright Varghese Philip Supply Caroline Allix-Béguec Mohammed Shoukri Ruba Al-Omari Mais Herbawi Sahal Al-Hajoj
author_facet	Bright Varghese Philip Supply Caroline Allix-Béguec Mohammed Shoukri Ruba Al-Omari Mais Herbawi Sahal Al-Hajoj
author_sort	Bright Varghese
title	Admixed phylogenetic distribution of drug resistant Mycobacterium tuberculosis in Saudi Arabia.
title_short	Admixed phylogenetic distribution of drug resistant Mycobacterium tuberculosis in Saudi Arabia.
title_full	Admixed phylogenetic distribution of drug resistant Mycobacterium tuberculosis in Saudi Arabia.
title_fullStr	Admixed phylogenetic distribution of drug resistant Mycobacterium tuberculosis in Saudi Arabia.
title_full_unstemmed	Admixed phylogenetic distribution of drug resistant Mycobacterium tuberculosis in Saudi Arabia.
title_sort	admixed phylogenetic distribution of drug resistant mycobacterium tuberculosis in saudi arabia.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/9da2cb1bec7f42d1a56fb8389e0e137f
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_version_	1718422684759490560

Admixed phylogenetic distribution of drug resistant Mycobacterium tuberculosis in Saudi Arabia.

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