Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>)

Loss of myofibers during muscle atrophy affects functional capacity and quality of life. Dexamethasone, an inducer of rapid atrophy of skeletal myofibers, has been studied as a glucocorticoid receptor in muscle atrophy or motor neurodegeneration. In this study, we examined dexamethasone-induced musc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bomi Ryu, Jun-Geon Je, You-Jin Jeon, Hye-Won Yang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	zebrafish muscle atrophy dexamethasone maca Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9da6aa82e1db4a4c85779486da71aa5c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9da6aa82e1db4a4c85779486da71aa5c
record_format	dspace
spelling	oai:doaj.org-article:9da6aa82e1db4a4c85779486da71aa5c2021-11-25T17:08:28ZZebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>)10.3390/cells101128792073-4409https://doaj.org/article/9da6aa82e1db4a4c85779486da71aa5c2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2879https://doaj.org/toc/2073-4409Loss of myofibers during muscle atrophy affects functional capacity and quality of life. Dexamethasone, an inducer of rapid atrophy of skeletal myofibers, has been studied as a glucocorticoid receptor in muscle atrophy or motor neurodegeneration. In this study, we examined dexamethasone-induced muscle atrophy using zebrafish (<i>Danio rerio</i>), a vertebrate model, and assessed whether administration of <i>Lepidium meyenii</i> (maca) as a dietary supplement can prevent muscle atrophy. Changes in skeletal myofibers in zebrafish were evaluated after exposure to dexamethasone for different periods and at different concentrations. Under optimized conditions, zebrafish pre-fed with maca for 3 days were exposed to 0.01% dexamethasone for 1 h/day for 7 days. Thereafter, myofiber loss, damaged muscle contractile proteins, and abnormal exploratory behavior due to the structural and functional impairment of skeletal muscle associated with muscle atrophy were investigated using hematoxylin–eosin, immunofluorescence staining, and behavioral analyses. Our findings suggest that dexamethasone induces muscle atrophy in zebrafish, inhibiting exploratory behavior by inducing myofiber loss, inhibiting muscle contraction, and causing changes in endurance and velocity. Thus, the zebrafish model can be used to screen pharmaceutical agents and to study muscle atrophy. Furthermore, maca is a potential dietary supplement to prevent muscle atrophy, as it protects muscle fibers.Bomi RyuJun-Geon JeYou-Jin JeonHye-Won YangMDPI AGarticlezebrafishmuscle atrophydexamethasonemacaBiology (General)QH301-705.5ENCells, Vol 10, Iss 2879, p 2879 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	zebrafish muscle atrophy dexamethasone maca Biology (General) QH301-705.5
spellingShingle	zebrafish muscle atrophy dexamethasone maca Biology (General) QH301-705.5 Bomi Ryu Jun-Geon Je You-Jin Jeon Hye-Won Yang Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>)
description	Loss of myofibers during muscle atrophy affects functional capacity and quality of life. Dexamethasone, an inducer of rapid atrophy of skeletal myofibers, has been studied as a glucocorticoid receptor in muscle atrophy or motor neurodegeneration. In this study, we examined dexamethasone-induced muscle atrophy using zebrafish (<i>Danio rerio</i>), a vertebrate model, and assessed whether administration of <i>Lepidium meyenii</i> (maca) as a dietary supplement can prevent muscle atrophy. Changes in skeletal myofibers in zebrafish were evaluated after exposure to dexamethasone for different periods and at different concentrations. Under optimized conditions, zebrafish pre-fed with maca for 3 days were exposed to 0.01% dexamethasone for 1 h/day for 7 days. Thereafter, myofiber loss, damaged muscle contractile proteins, and abnormal exploratory behavior due to the structural and functional impairment of skeletal muscle associated with muscle atrophy were investigated using hematoxylin–eosin, immunofluorescence staining, and behavioral analyses. Our findings suggest that dexamethasone induces muscle atrophy in zebrafish, inhibiting exploratory behavior by inducing myofiber loss, inhibiting muscle contraction, and causing changes in endurance and velocity. Thus, the zebrafish model can be used to screen pharmaceutical agents and to study muscle atrophy. Furthermore, maca is a potential dietary supplement to prevent muscle atrophy, as it protects muscle fibers.
format	article
author	Bomi Ryu Jun-Geon Je You-Jin Jeon Hye-Won Yang
author_facet	Bomi Ryu Jun-Geon Je You-Jin Jeon Hye-Won Yang
author_sort	Bomi Ryu
title	Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>)
title_short	Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>)
title_full	Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>)
title_fullStr	Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>)
title_full_unstemmed	Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>)
title_sort	zebrafish model for studying dexamethasone-induced muscle atrophy and preventive effect of maca (<i>lepidium meyenii</i>)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9da6aa82e1db4a4c85779486da71aa5c
work_keys_str_mv	AT bomiryu zebrafishmodelforstudyingdexamethasoneinducedmuscleatrophyandpreventiveeffectofmacailepidiummeyeniii AT jungeonje zebrafishmodelforstudyingdexamethasoneinducedmuscleatrophyandpreventiveeffectofmacailepidiummeyeniii AT youjinjeon zebrafishmodelforstudyingdexamethasoneinducedmuscleatrophyandpreventiveeffectofmacailepidiummeyeniii AT hyewonyang zebrafishmodelforstudyingdexamethasoneinducedmuscleatrophyandpreventiveeffectofmacailepidiummeyeniii
_version_	1718412729135398912

Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>)

Ejemplares similares