Polysaccharide-based nanoparticles for co-loading mitoxantrone and verapamil to overcome multidrug resistance in breast tumor

Yurui Xu,1 Sajid Asghar,2 Shiya Gao,1 Zhipeng Chen,3 Lin Huang,1 Lining Yin,1 Qineng Ping,1 Yanyu Xiao11Department of Pharmaceutics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, People’s Republic of China; 2Faculty of Pharmaceutical Sciences, Governm...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xu Y, Asghar S, Gao S, Chen Z, Huang L, Yin L, Ping Q, Xiao Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Hyaluronic acid Chitosan hydrochloride Mitoxantrone hydrochloride Verapamil hydrochloride Co-delivery Multi-drug resistance. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9daa0c4e84564252b6cd94fed69cd323
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9daa0c4e84564252b6cd94fed69cd323
record_format	dspace
spelling	oai:doaj.org-article:9daa0c4e84564252b6cd94fed69cd3232021-12-02T01:34:04ZPolysaccharide-based nanoparticles for co-loading mitoxantrone and verapamil to overcome multidrug resistance in breast tumor1178-2013https://doaj.org/article/9daa0c4e84564252b6cd94fed69cd3232017-10-01T00:00:00Zhttps://www.dovepress.com/polysaccharide-based-nanoparticles-for-co-loading-mitoxantrone-and-ver-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yurui Xu,1 Sajid Asghar,2 Shiya Gao,1 Zhipeng Chen,3 Lin Huang,1 Lining Yin,1 Qineng Ping,1 Yanyu Xiao11Department of Pharmaceutics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, People’s Republic of China; 2Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad, Pakistan; 3Department of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People’s Republic of China Abstract: The aim of this study was to evaluate the potential of polyelectrolyte complex nanoparticles (PENPs) based on hyaluronic acid/chitosan hydrochloride (HA/HCS) for co-loading mitoxantrone (MTO) and verapamil (VRP) to overcome multidrug resistance in breast tumors. PENPs co-loaded with MTO and VRP (MTO-VRP-PENPs) were affected by the method of preparation, molecular weight of HA, mass ratios and initial concentrations of HA/HCS, pH, and drug quantities. Optimized MTO-VRP-PENPs were ~209 nm in size with a zeta potential of approximately −24 mV. Encapsulation efficiencies (%) of MTO and VRP were 98.33%±0.27% and 44.21%±8.62%, respectively. MTO and VRP were successfully encapsulated in PENPs in a molecular or amorphous state. MTO-VRP-PENPs showed significant cytotoxicity in MCF-7/ADR cells in contrast to MTO-loaded PENPs (MTO-PENPs). The reversal index of MTO-VRP-PENPs was 13.25 and 10.33 times greater than that of the free MTO and MTO-PENPs, respectively. In conclusion, MTO-VRP-PENPs may serve as a promising carrier to overcome tumor drug resistance.Keywords: hyaluronic acid, chitosan hydrochloride, mitoxantrone hydrochloride, verapamil hydrochloride, co-delivery, multidrug resistanceXu YAsghar SGao SChen ZHuang LYin LPing QXiao YDove Medical PressarticleHyaluronic acidChitosan hydrochlorideMitoxantrone hydrochlorideVerapamil hydrochlorideCo-deliveryMulti-drug resistance.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7337-7350 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hyaluronic acid Chitosan hydrochloride Mitoxantrone hydrochloride Verapamil hydrochloride Co-delivery Multi-drug resistance. Medicine (General) R5-920
spellingShingle	Hyaluronic acid Chitosan hydrochloride Mitoxantrone hydrochloride Verapamil hydrochloride Co-delivery Multi-drug resistance. Medicine (General) R5-920 Xu Y Asghar S Gao S Chen Z Huang L Yin L Ping Q Xiao Y Polysaccharide-based nanoparticles for co-loading mitoxantrone and verapamil to overcome multidrug resistance in breast tumor
description	Yurui Xu,1 Sajid Asghar,2 Shiya Gao,1 Zhipeng Chen,3 Lin Huang,1 Lining Yin,1 Qineng Ping,1 Yanyu Xiao11Department of Pharmaceutics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, People’s Republic of China; 2Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad, Pakistan; 3Department of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People’s Republic of China Abstract: The aim of this study was to evaluate the potential of polyelectrolyte complex nanoparticles (PENPs) based on hyaluronic acid/chitosan hydrochloride (HA/HCS) for co-loading mitoxantrone (MTO) and verapamil (VRP) to overcome multidrug resistance in breast tumors. PENPs co-loaded with MTO and VRP (MTO-VRP-PENPs) were affected by the method of preparation, molecular weight of HA, mass ratios and initial concentrations of HA/HCS, pH, and drug quantities. Optimized MTO-VRP-PENPs were ~209 nm in size with a zeta potential of approximately −24 mV. Encapsulation efficiencies (%) of MTO and VRP were 98.33%±0.27% and 44.21%±8.62%, respectively. MTO and VRP were successfully encapsulated in PENPs in a molecular or amorphous state. MTO-VRP-PENPs showed significant cytotoxicity in MCF-7/ADR cells in contrast to MTO-loaded PENPs (MTO-PENPs). The reversal index of MTO-VRP-PENPs was 13.25 and 10.33 times greater than that of the free MTO and MTO-PENPs, respectively. In conclusion, MTO-VRP-PENPs may serve as a promising carrier to overcome tumor drug resistance.Keywords: hyaluronic acid, chitosan hydrochloride, mitoxantrone hydrochloride, verapamil hydrochloride, co-delivery, multidrug resistance
format	article
author	Xu Y Asghar S Gao S Chen Z Huang L Yin L Ping Q Xiao Y
author_facet	Xu Y Asghar S Gao S Chen Z Huang L Yin L Ping Q Xiao Y
author_sort	Xu Y
title	Polysaccharide-based nanoparticles for co-loading mitoxantrone and verapamil to overcome multidrug resistance in breast tumor
title_short	Polysaccharide-based nanoparticles for co-loading mitoxantrone and verapamil to overcome multidrug resistance in breast tumor
title_full	Polysaccharide-based nanoparticles for co-loading mitoxantrone and verapamil to overcome multidrug resistance in breast tumor
title_fullStr	Polysaccharide-based nanoparticles for co-loading mitoxantrone and verapamil to overcome multidrug resistance in breast tumor
title_full_unstemmed	Polysaccharide-based nanoparticles for co-loading mitoxantrone and verapamil to overcome multidrug resistance in breast tumor
title_sort	polysaccharide-based nanoparticles for co-loading mitoxantrone and verapamil to overcome multidrug resistance in breast tumor
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/9daa0c4e84564252b6cd94fed69cd323
work_keys_str_mv	AT xuy polysaccharidebasednanoparticlesforcoloadingmitoxantroneandverapamiltoovercomemultidrugresistanceinbreasttumor AT asghars polysaccharidebasednanoparticlesforcoloadingmitoxantroneandverapamiltoovercomemultidrugresistanceinbreasttumor AT gaos polysaccharidebasednanoparticlesforcoloadingmitoxantroneandverapamiltoovercomemultidrugresistanceinbreasttumor AT chenz polysaccharidebasednanoparticlesforcoloadingmitoxantroneandverapamiltoovercomemultidrugresistanceinbreasttumor AT huangl polysaccharidebasednanoparticlesforcoloadingmitoxantroneandverapamiltoovercomemultidrugresistanceinbreasttumor AT yinl polysaccharidebasednanoparticlesforcoloadingmitoxantroneandverapamiltoovercomemultidrugresistanceinbreasttumor AT pingq polysaccharidebasednanoparticlesforcoloadingmitoxantroneandverapamiltoovercomemultidrugresistanceinbreasttumor AT xiaoy polysaccharidebasednanoparticlesforcoloadingmitoxantroneandverapamiltoovercomemultidrugresistanceinbreasttumor
_version_	1718403030387261440

Polysaccharide-based nanoparticles for co-loading mitoxantrone and verapamil to overcome multidrug resistance in breast tumor

Ejemplares similares