Neochlorogenic acid: an anti-HIV active compound identified by screening of Cortex Mori [Morus Alba L. (Moraceae)]

Context Chinese herbs such as Cortex Mori [Morus alba L. (Moraceae)] may inhibit human immunodeficiency virus (HIV), but active compounds are unknown. Objective Screening of Cortex Mori and other herbs for anti-HIV active compounds. Materials and methods HIV-1 virus (multiplicity of infection: 20),...

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Autores principales:	Jing Li, Lu Dou, Shuangfeng Chen, Honghao Zhou, Fangzheng Mou
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	traditional chinese medicine reverse transcriptase enrichment analysis Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/9dad1c7ac2c2414c9b1b9ec9ae49ffa1
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spelling	oai:doaj.org-article:9dad1c7ac2c2414c9b1b9ec9ae49ffa12021-11-04T15:00:42ZNeochlorogenic acid: an anti-HIV active compound identified by screening of Cortex Mori [Morus Alba L. (Moraceae)]1388-02091744-511610.1080/13880209.2021.1995005https://doaj.org/article/9dad1c7ac2c2414c9b1b9ec9ae49ffa12021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2021.1995005https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context Chinese herbs such as Cortex Mori [Morus alba L. (Moraceae)] may inhibit human immunodeficiency virus (HIV), but active compounds are unknown. Objective Screening of Cortex Mori and other herbs for anti-HIV active compounds. Materials and methods HIV-1 virus (multiplicity of infection: 20), and herbs (dissolved in dimethyl sulfoxide, working concentrations: 10, 1, and 0.1 mg/mL) such as Cortex Mori, etc., were added to 786-O cells (105 cell/well). Zidovudine was used as a positive control. Cell survival and viral inhibition rates were measured. The herb that was the closest inactivity to zidovudine was screened. Mass spectrometry identified the active compounds in herbs (mobile phase: 0.05% formic acid aqueous solution and acetonitrile, gradient elution, detection wavelength: 210 nm). The effect of the compounds on reverse transcriptase (RT) products were evaluated by real-time PCR. Gene enrichment was used to analyse underlying mechanisms. Results With a dose of 1 mg/mL of Cortex Mori, the cell survival rate (57.94%) and viral inhibition rate (74.95%) were closest to the effect of zidovudine (87.87%, 79.81%, respectively). Neochlorogenic acid, one of the active ingredients, was identified by mass spectrometry in Cortex Mori. PCR discovery total RT products of neochlorogenic acid group (mean relative gene expression: 6.01) significantly inhibited (control: 35.42, p < 0.0001). Enrichment analysis showed that neochlorogenic acid may act on haemopoietic cell kinase, epidermal growth factor receptor, sarcoma, etc., thus inhibiting HIV-1 infection. Conclusions For people of low socioeconomic status affected by HIV, Chinese medicine (such as Cortex Mori) has many advantages: it is inexpensive and does not easily produce resistance. Drugs based on active ingredients may be developed and could have important value.Jing LiLu DouShuangfeng ChenHonghao ZhouFangzheng MouTaylor & Francis Grouparticletraditional chinese medicinereverse transcriptaseenrichment analysisTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 59, Iss 1, Pp 1517-1527 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	traditional chinese medicine reverse transcriptase enrichment analysis Therapeutics. Pharmacology RM1-950
spellingShingle	traditional chinese medicine reverse transcriptase enrichment analysis Therapeutics. Pharmacology RM1-950 Jing Li Lu Dou Shuangfeng Chen Honghao Zhou Fangzheng Mou Neochlorogenic acid: an anti-HIV active compound identified by screening of Cortex Mori [Morus Alba L. (Moraceae)]
description	Context Chinese herbs such as Cortex Mori [Morus alba L. (Moraceae)] may inhibit human immunodeficiency virus (HIV), but active compounds are unknown. Objective Screening of Cortex Mori and other herbs for anti-HIV active compounds. Materials and methods HIV-1 virus (multiplicity of infection: 20), and herbs (dissolved in dimethyl sulfoxide, working concentrations: 10, 1, and 0.1 mg/mL) such as Cortex Mori, etc., were added to 786-O cells (105 cell/well). Zidovudine was used as a positive control. Cell survival and viral inhibition rates were measured. The herb that was the closest inactivity to zidovudine was screened. Mass spectrometry identified the active compounds in herbs (mobile phase: 0.05% formic acid aqueous solution and acetonitrile, gradient elution, detection wavelength: 210 nm). The effect of the compounds on reverse transcriptase (RT) products were evaluated by real-time PCR. Gene enrichment was used to analyse underlying mechanisms. Results With a dose of 1 mg/mL of Cortex Mori, the cell survival rate (57.94%) and viral inhibition rate (74.95%) were closest to the effect of zidovudine (87.87%, 79.81%, respectively). Neochlorogenic acid, one of the active ingredients, was identified by mass spectrometry in Cortex Mori. PCR discovery total RT products of neochlorogenic acid group (mean relative gene expression: 6.01) significantly inhibited (control: 35.42, p < 0.0001). Enrichment analysis showed that neochlorogenic acid may act on haemopoietic cell kinase, epidermal growth factor receptor, sarcoma, etc., thus inhibiting HIV-1 infection. Conclusions For people of low socioeconomic status affected by HIV, Chinese medicine (such as Cortex Mori) has many advantages: it is inexpensive and does not easily produce resistance. Drugs based on active ingredients may be developed and could have important value.
format	article
author	Jing Li Lu Dou Shuangfeng Chen Honghao Zhou Fangzheng Mou
author_facet	Jing Li Lu Dou Shuangfeng Chen Honghao Zhou Fangzheng Mou
author_sort	Jing Li
title	Neochlorogenic acid: an anti-HIV active compound identified by screening of Cortex Mori [Morus Alba L. (Moraceae)]
title_short	Neochlorogenic acid: an anti-HIV active compound identified by screening of Cortex Mori [Morus Alba L. (Moraceae)]
title_full	Neochlorogenic acid: an anti-HIV active compound identified by screening of Cortex Mori [Morus Alba L. (Moraceae)]
title_fullStr	Neochlorogenic acid: an anti-HIV active compound identified by screening of Cortex Mori [Morus Alba L. (Moraceae)]
title_full_unstemmed	Neochlorogenic acid: an anti-HIV active compound identified by screening of Cortex Mori [Morus Alba L. (Moraceae)]
title_sort	neochlorogenic acid: an anti-hiv active compound identified by screening of cortex mori [morus alba l. (moraceae)]
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/9dad1c7ac2c2414c9b1b9ec9ae49ffa1
work_keys_str_mv	AT jingli neochlorogenicacidanantihivactivecompoundidentifiedbyscreeningofcortexmorimorusalbalmoraceae AT ludou neochlorogenicacidanantihivactivecompoundidentifiedbyscreeningofcortexmorimorusalbalmoraceae AT shuangfengchen neochlorogenicacidanantihivactivecompoundidentifiedbyscreeningofcortexmorimorusalbalmoraceae AT honghaozhou neochlorogenicacidanantihivactivecompoundidentifiedbyscreeningofcortexmorimorusalbalmoraceae AT fangzhengmou neochlorogenicacidanantihivactivecompoundidentifiedbyscreeningofcortexmorimorusalbalmoraceae
_version_	1718444789010006016

Neochlorogenic acid: an anti-HIV active compound identified by screening of Cortex Mori [Morus Alba L. (Moraceae)]

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