Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model

Abstract Temporal lobe epilepsy (TLE) is the most common epilepsy type. TLE onset in infancy aggravates features like severity, drug responsiveness, or development of comorbidities. These aggravations may arise from altered micro RNA (miRNA) expression specific to the early onset of the disease. Alt...

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Autores principales:	Petra Bencurova, Jiri Baloun, Jakub Hynst, Jan Oppelt, Hana Kubova, Sarka Pospisilova, Milan Brazdil
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9db2b901db6249209f30744cba479bd3
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spelling	oai:doaj.org-article:9db2b901db6249209f30744cba479bd32021-12-02T15:38:23ZDynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model10.1038/s41598-021-89084-92045-2322https://doaj.org/article/9db2b901db6249209f30744cba479bd32021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89084-9https://doaj.org/toc/2045-2322Abstract Temporal lobe epilepsy (TLE) is the most common epilepsy type. TLE onset in infancy aggravates features like severity, drug responsiveness, or development of comorbidities. These aggravations may arise from altered micro RNA (miRNA) expression specific to the early onset of the disease. Although the miRNA involvement in TLE is widely studied, the relationship between the onset-age and miRNA expression has not been addressed. Here, we investigated the miRNA profile of infantile and adult-onset TLE in rats combining sequencing and PCR. Since miRNA expression changes with the disease progression, we scrutinized miRNA dynamics across three stages: acute, latent, and chronic. We report that infantile-onset TLE leads to changes in the expression of fewer miRNAs across these stages. Interestingly, the miRNA profile in the acute stage of infantile-onset TLE overlaps in dysregulation of miR-132-5p, -205, and -211-3p with the chronic stage of the disease starting in adulthood. The analysis of putative targets linked the majority of dysregulated miRNAs with pathways involved in epilepsy. Our profiling uncovered miRNA expression characteristic for infantile and adulthood-onset epileptogenesis, suggesting the distinct biology underlying TLE in the onset age-dependent matter. Our results indicate the necessity of addressing the onset age as an important parameter in future epilepsy research.Petra BencurovaJiri BalounJakub HynstJan OppeltHana KubovaSarka PospisilovaMilan BrazdilNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Petra Bencurova Jiri Baloun Jakub Hynst Jan Oppelt Hana Kubova Sarka Pospisilova Milan Brazdil Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model
description	Abstract Temporal lobe epilepsy (TLE) is the most common epilepsy type. TLE onset in infancy aggravates features like severity, drug responsiveness, or development of comorbidities. These aggravations may arise from altered micro RNA (miRNA) expression specific to the early onset of the disease. Although the miRNA involvement in TLE is widely studied, the relationship between the onset-age and miRNA expression has not been addressed. Here, we investigated the miRNA profile of infantile and adult-onset TLE in rats combining sequencing and PCR. Since miRNA expression changes with the disease progression, we scrutinized miRNA dynamics across three stages: acute, latent, and chronic. We report that infantile-onset TLE leads to changes in the expression of fewer miRNAs across these stages. Interestingly, the miRNA profile in the acute stage of infantile-onset TLE overlaps in dysregulation of miR-132-5p, -205, and -211-3p with the chronic stage of the disease starting in adulthood. The analysis of putative targets linked the majority of dysregulated miRNAs with pathways involved in epilepsy. Our profiling uncovered miRNA expression characteristic for infantile and adulthood-onset epileptogenesis, suggesting the distinct biology underlying TLE in the onset age-dependent matter. Our results indicate the necessity of addressing the onset age as an important parameter in future epilepsy research.
format	article
author	Petra Bencurova Jiri Baloun Jakub Hynst Jan Oppelt Hana Kubova Sarka Pospisilova Milan Brazdil
author_facet	Petra Bencurova Jiri Baloun Jakub Hynst Jan Oppelt Hana Kubova Sarka Pospisilova Milan Brazdil
author_sort	Petra Bencurova
title	Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model
title_short	Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model
title_full	Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model
title_fullStr	Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model
title_full_unstemmed	Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model
title_sort	dynamic mirna changes during the process of epileptogenesis in an infantile and adult-onset model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9db2b901db6249209f30744cba479bd3
work_keys_str_mv	AT petrabencurova dynamicmirnachangesduringtheprocessofepileptogenesisinaninfantileandadultonsetmodel AT jiribaloun dynamicmirnachangesduringtheprocessofepileptogenesisinaninfantileandadultonsetmodel AT jakubhynst dynamicmirnachangesduringtheprocessofepileptogenesisinaninfantileandadultonsetmodel AT janoppelt dynamicmirnachangesduringtheprocessofepileptogenesisinaninfantileandadultonsetmodel AT hanakubova dynamicmirnachangesduringtheprocessofepileptogenesisinaninfantileandadultonsetmodel AT sarkapospisilova dynamicmirnachangesduringtheprocessofepileptogenesisinaninfantileandadultonsetmodel AT milanbrazdil dynamicmirnachangesduringtheprocessofepileptogenesisinaninfantileandadultonsetmodel
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Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model

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