A new primer pair for barcoding of bees (Hymenoptera: Anthophila) without amplifying the orthologous coxA gene of Wolbachia bacteria

Abstract Objectives DNA barcoding became an effective method for the identification and monitoring of bees. However, standard primer pairs used for barcoding often result in (co-) amplification of bacterial endosymbionts of the genus Wolbachia, which are widespread among bee species. Here we designe...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Christoph Bleidorn, Katharina Henze
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Apoidea Biomonitoring DNA barcoding Misamplification Medicine R Biology (General) QH301-705.5 Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/9db4fb8f93b5469cb1f3d24e3ddb0606
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9db4fb8f93b5469cb1f3d24e3ddb0606
record_format	dspace
spelling	oai:doaj.org-article:9db4fb8f93b5469cb1f3d24e3ddb06062021-11-28T12:24:57ZA new primer pair for barcoding of bees (Hymenoptera: Anthophila) without amplifying the orthologous coxA gene of Wolbachia bacteria10.1186/s13104-021-05845-91756-0500https://doaj.org/article/9db4fb8f93b5469cb1f3d24e3ddb06062021-11-01T00:00:00Zhttps://doi.org/10.1186/s13104-021-05845-9https://doaj.org/toc/1756-0500Abstract Objectives DNA barcoding became an effective method for the identification and monitoring of bees. However, standard primer pairs used for barcoding often result in (co-) amplification of bacterial endosymbionts of the genus Wolbachia, which are widespread among bee species. Here we designed a new primer pair and compared it with the performance of the standard Folmer-primers for a small sample set of bees representing the main taxonomic groups of bees. Results The newly designed primer pair (BeeCox1F1/BeeCox1R2) outperformed the standard barcoding primer (LCO1490/HCO2198). By generating barcodes for a small test set of bees we found that the new primer pair produced high-quality sequences in all cases for unambiguous species identification using BOLD. Conversely, the standard barcoding primers often co-amplified the homologous Wolbachia gene and resulted in mixed chromatogram signals. These sequences showed high similarity with the bacterial endosymbiont instead of the host.Christoph BleidornKatharina HenzeBMCarticleApoideaBiomonitoringDNA barcodingMisamplificationMedicineRBiology (General)QH301-705.5Science (General)Q1-390ENBMC Research Notes, Vol 14, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Apoidea Biomonitoring DNA barcoding Misamplification Medicine R Biology (General) QH301-705.5 Science (General) Q1-390
spellingShingle	Apoidea Biomonitoring DNA barcoding Misamplification Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 Christoph Bleidorn Katharina Henze A new primer pair for barcoding of bees (Hymenoptera: Anthophila) without amplifying the orthologous coxA gene of Wolbachia bacteria
description	Abstract Objectives DNA barcoding became an effective method for the identification and monitoring of bees. However, standard primer pairs used for barcoding often result in (co-) amplification of bacterial endosymbionts of the genus Wolbachia, which are widespread among bee species. Here we designed a new primer pair and compared it with the performance of the standard Folmer-primers for a small sample set of bees representing the main taxonomic groups of bees. Results The newly designed primer pair (BeeCox1F1/BeeCox1R2) outperformed the standard barcoding primer (LCO1490/HCO2198). By generating barcodes for a small test set of bees we found that the new primer pair produced high-quality sequences in all cases for unambiguous species identification using BOLD. Conversely, the standard barcoding primers often co-amplified the homologous Wolbachia gene and resulted in mixed chromatogram signals. These sequences showed high similarity with the bacterial endosymbiont instead of the host.
format	article
author	Christoph Bleidorn Katharina Henze
author_facet	Christoph Bleidorn Katharina Henze
author_sort	Christoph Bleidorn
title	A new primer pair for barcoding of bees (Hymenoptera: Anthophila) without amplifying the orthologous coxA gene of Wolbachia bacteria
title_short	A new primer pair for barcoding of bees (Hymenoptera: Anthophila) without amplifying the orthologous coxA gene of Wolbachia bacteria
title_full	A new primer pair for barcoding of bees (Hymenoptera: Anthophila) without amplifying the orthologous coxA gene of Wolbachia bacteria
title_fullStr	A new primer pair for barcoding of bees (Hymenoptera: Anthophila) without amplifying the orthologous coxA gene of Wolbachia bacteria
title_full_unstemmed	A new primer pair for barcoding of bees (Hymenoptera: Anthophila) without amplifying the orthologous coxA gene of Wolbachia bacteria
title_sort	new primer pair for barcoding of bees (hymenoptera: anthophila) without amplifying the orthologous coxa gene of wolbachia bacteria
publisher	BMC
publishDate	2021
url	https://doaj.org/article/9db4fb8f93b5469cb1f3d24e3ddb0606
work_keys_str_mv	AT christophbleidorn anewprimerpairforbarcodingofbeeshymenopteraanthophilawithoutamplifyingtheorthologouscoxageneofwolbachiabacteria AT katharinahenze anewprimerpairforbarcodingofbeeshymenopteraanthophilawithoutamplifyingtheorthologouscoxageneofwolbachiabacteria AT christophbleidorn newprimerpairforbarcodingofbeeshymenopteraanthophilawithoutamplifyingtheorthologouscoxageneofwolbachiabacteria AT katharinahenze newprimerpairforbarcodingofbeeshymenopteraanthophilawithoutamplifyingtheorthologouscoxageneofwolbachiabacteria
_version_	1718407972841848832

A new primer pair for barcoding of bees (Hymenoptera: Anthophila) without amplifying the orthologous coxA gene of Wolbachia bacteria

Ejemplares similares