Long-term diet-induced obesity does not lead to learning and memory impairment in adult mice.

Obesity arising from excessive dietary fat intake is a risk factor for cognitive decline, dementia and neurodegenerative diseases, including Alzheimer's disease. Here, we studied the effect of long-term high-fat diet (HFD) (24 weeks) and return to normal diet (ND) on behavioral features, microg...

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Autores principales:	Judith Leyh, Karsten Winter, Madlen Reinicke, Uta Ceglarek, Ingo Bechmann, Julia Landmann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9dbdddb33a9546b8b3e90ed4550bd120
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spelling	oai:doaj.org-article:9dbdddb33a9546b8b3e90ed4550bd1202021-12-02T20:13:59ZLong-term diet-induced obesity does not lead to learning and memory impairment in adult mice.1932-620310.1371/journal.pone.0257921https://doaj.org/article/9dbdddb33a9546b8b3e90ed4550bd1202021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257921https://doaj.org/toc/1932-6203Obesity arising from excessive dietary fat intake is a risk factor for cognitive decline, dementia and neurodegenerative diseases, including Alzheimer's disease. Here, we studied the effect of long-term high-fat diet (HFD) (24 weeks) and return to normal diet (ND) on behavioral features, microglia and neurons in adult male C57BL/6J mice. Consequences of HFD-induced obesity and dietary changes on general health (coat appearance, presence of vibrissae), sensory and motor reflexes, learning and memory were assessed by applying a phenotypic assessment protocol, the Y maze and Morris Water Maze test. Neurons and microglia were histologically analyzed within the mediobasal hypothalamus, hippocampus and frontal motor cortex after long-term HFD and change of diet. Long periods of HFD caused general health issues (coat alterations, loss of vibrissae), but did not affect sensory and motor reflexes, emotional state, memory and learning. Long-term HFD increased the microglial response (increased Iba1 fluorescence intensity, percentage of Iba1-stained area and Iba1 gene expression) within the hypothalamus, but not in the cortex and hippocampus. In neither of these regions, neurodegeneration or intracellular lipid droplet accumulation was observed. The former alterations were reversible in mice whose diet was changed from HFD to ND. Taken together, long periods of excessive dietary fat alone do not cause learning deficits or spatial memory impairment, though HFD-induced obesity may have detrimental consequences for cognitive flexibility. Our data confirm the selective responsiveness of hypothalamic microglia to HFD.Judith LeyhKarsten WinterMadlen ReinickeUta CeglarekIngo BechmannJulia LandmannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257921 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Judith Leyh Karsten Winter Madlen Reinicke Uta Ceglarek Ingo Bechmann Julia Landmann Long-term diet-induced obesity does not lead to learning and memory impairment in adult mice.
description	Obesity arising from excessive dietary fat intake is a risk factor for cognitive decline, dementia and neurodegenerative diseases, including Alzheimer's disease. Here, we studied the effect of long-term high-fat diet (HFD) (24 weeks) and return to normal diet (ND) on behavioral features, microglia and neurons in adult male C57BL/6J mice. Consequences of HFD-induced obesity and dietary changes on general health (coat appearance, presence of vibrissae), sensory and motor reflexes, learning and memory were assessed by applying a phenotypic assessment protocol, the Y maze and Morris Water Maze test. Neurons and microglia were histologically analyzed within the mediobasal hypothalamus, hippocampus and frontal motor cortex after long-term HFD and change of diet. Long periods of HFD caused general health issues (coat alterations, loss of vibrissae), but did not affect sensory and motor reflexes, emotional state, memory and learning. Long-term HFD increased the microglial response (increased Iba1 fluorescence intensity, percentage of Iba1-stained area and Iba1 gene expression) within the hypothalamus, but not in the cortex and hippocampus. In neither of these regions, neurodegeneration or intracellular lipid droplet accumulation was observed. The former alterations were reversible in mice whose diet was changed from HFD to ND. Taken together, long periods of excessive dietary fat alone do not cause learning deficits or spatial memory impairment, though HFD-induced obesity may have detrimental consequences for cognitive flexibility. Our data confirm the selective responsiveness of hypothalamic microglia to HFD.
format	article
author	Judith Leyh Karsten Winter Madlen Reinicke Uta Ceglarek Ingo Bechmann Julia Landmann
author_facet	Judith Leyh Karsten Winter Madlen Reinicke Uta Ceglarek Ingo Bechmann Julia Landmann
author_sort	Judith Leyh
title	Long-term diet-induced obesity does not lead to learning and memory impairment in adult mice.
title_short	Long-term diet-induced obesity does not lead to learning and memory impairment in adult mice.
title_full	Long-term diet-induced obesity does not lead to learning and memory impairment in adult mice.
title_fullStr	Long-term diet-induced obesity does not lead to learning and memory impairment in adult mice.
title_full_unstemmed	Long-term diet-induced obesity does not lead to learning and memory impairment in adult mice.
title_sort	long-term diet-induced obesity does not lead to learning and memory impairment in adult mice.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/9dbdddb33a9546b8b3e90ed4550bd120
work_keys_str_mv	AT judithleyh longtermdietinducedobesitydoesnotleadtolearningandmemoryimpairmentinadultmice AT karstenwinter longtermdietinducedobesitydoesnotleadtolearningandmemoryimpairmentinadultmice AT madlenreinicke longtermdietinducedobesitydoesnotleadtolearningandmemoryimpairmentinadultmice AT utaceglarek longtermdietinducedobesitydoesnotleadtolearningandmemoryimpairmentinadultmice AT ingobechmann longtermdietinducedobesitydoesnotleadtolearningandmemoryimpairmentinadultmice AT julialandmann longtermdietinducedobesitydoesnotleadtolearningandmemoryimpairmentinadultmice
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Long-term diet-induced obesity does not lead to learning and memory impairment in adult mice.

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