Microbiome Analysis of Mucosal Ileoanal Pouch in Ulcerative Colitis Patients Revealed Impairment of the Pouches Immunometabolites

The pathogenesis of ulcerative colitis (UC) is unknown, although genetic loci and altered gut microbiota have been implicated. Up to a third of patients with moderate to severe UC require proctocolectomy with ileal pouch ano-anastomosis (IPAA). We aimed to explore the mucosal microbiota of UC patien...

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Autores principales:	Orazio Palmieri, Stefano Castellana, Giuseppe Biscaglia, Anna Panza, Anna Latiano, Rosanna Fontana, Maria Guerra, Giuseppe Corritore, Tiziana Latiano, Giuseppina Martino, Tommaso Mazza, Angelo Andriulli, Francesco Perri, Fabrizio Bossa
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ulcerative colitis (UC) IBD IPAA pouch pouchitis microbiota Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9dbf4f34c366414090a026ca87cc603f
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spelling	oai:doaj.org-article:9dbf4f34c366414090a026ca87cc603f2021-11-25T17:13:13ZMicrobiome Analysis of Mucosal Ileoanal Pouch in Ulcerative Colitis Patients Revealed Impairment of the Pouches Immunometabolites10.3390/cells101132432073-4409https://doaj.org/article/9dbf4f34c366414090a026ca87cc603f2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3243https://doaj.org/toc/2073-4409The pathogenesis of ulcerative colitis (UC) is unknown, although genetic loci and altered gut microbiota have been implicated. Up to a third of patients with moderate to severe UC require proctocolectomy with ileal pouch ano-anastomosis (IPAA). We aimed to explore the mucosal microbiota of UC patients who underwent IPAA. Methods: For microbiome analysis, mucosal specimens were collected from 34 IPAA individuals. Endoscopic and histological examinations of IPAA were normal in 21 cases, while pouchitis was in 13 patients. 19 specimens from the healthy control (10 from colonic and 9 from ileum) were also analyzed. Data were analyzed using an ensemble of software packages: QIIME2, coda-lasso, clr-lasso, PICRUSt2, and ALDEx2. Results: IPAA specimens had significantly lower bacterial diversity as compared to normal. The microbial composition of the normal pouch was also decreased also when compared to pouchitis. <i>Faecalibacterium prausnitzii</i>, <i>Gemmiger formicilis</i>, <i>Blautia obeum</i>, <i>Ruminococcus torques</i>, <i>Dorea formicigenerans</i>, and an unknown species from <i>Roseburia</i> were the most uncommon in pouch/pouchitis, while an unknown species from <i>Enterobacteriaceae</i> was over-represented. <i>Propionibacterium acnes</i> and <i>Enterobacteriaceae</i> were the species most abundant in the pouchitis and in the normal pouch, respectively. Predicted metabolic pathways among the IPAA bacterial communities revealed an important role of immunometabolites such as SCFA, butyrate, and amino acids. Conclusions: Our findings showed specific bacterial signature hallmarks of dysbiosis and could represent bacterial biomarkers in IPAA patients useful to develop novel treatments in the future by modulating the gut microbiota through the administration of probiotic immunometabolites-producing bacterial strains and the addition of specific prebiotics and the faecal microbiota transplantation.Orazio PalmieriStefano CastellanaGiuseppe BiscagliaAnna PanzaAnna LatianoRosanna FontanaMaria GuerraGiuseppe CorritoreTiziana LatianoGiuseppina MartinoTommaso MazzaAngelo AndriulliFrancesco PerriFabrizio BossaMDPI AGarticleulcerative colitis (UC)IBDIPAApouchpouchitismicrobiotaBiology (General)QH301-705.5ENCells, Vol 10, Iss 3243, p 3243 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ulcerative colitis (UC) IBD IPAA pouch pouchitis microbiota Biology (General) QH301-705.5
spellingShingle	ulcerative colitis (UC) IBD IPAA pouch pouchitis microbiota Biology (General) QH301-705.5 Orazio Palmieri Stefano Castellana Giuseppe Biscaglia Anna Panza Anna Latiano Rosanna Fontana Maria Guerra Giuseppe Corritore Tiziana Latiano Giuseppina Martino Tommaso Mazza Angelo Andriulli Francesco Perri Fabrizio Bossa Microbiome Analysis of Mucosal Ileoanal Pouch in Ulcerative Colitis Patients Revealed Impairment of the Pouches Immunometabolites
description	The pathogenesis of ulcerative colitis (UC) is unknown, although genetic loci and altered gut microbiota have been implicated. Up to a third of patients with moderate to severe UC require proctocolectomy with ileal pouch ano-anastomosis (IPAA). We aimed to explore the mucosal microbiota of UC patients who underwent IPAA. Methods: For microbiome analysis, mucosal specimens were collected from 34 IPAA individuals. Endoscopic and histological examinations of IPAA were normal in 21 cases, while pouchitis was in 13 patients. 19 specimens from the healthy control (10 from colonic and 9 from ileum) were also analyzed. Data were analyzed using an ensemble of software packages: QIIME2, coda-lasso, clr-lasso, PICRUSt2, and ALDEx2. Results: IPAA specimens had significantly lower bacterial diversity as compared to normal. The microbial composition of the normal pouch was also decreased also when compared to pouchitis. <i>Faecalibacterium prausnitzii</i>, <i>Gemmiger formicilis</i>, <i>Blautia obeum</i>, <i>Ruminococcus torques</i>, <i>Dorea formicigenerans</i>, and an unknown species from <i>Roseburia</i> were the most uncommon in pouch/pouchitis, while an unknown species from <i>Enterobacteriaceae</i> was over-represented. <i>Propionibacterium acnes</i> and <i>Enterobacteriaceae</i> were the species most abundant in the pouchitis and in the normal pouch, respectively. Predicted metabolic pathways among the IPAA bacterial communities revealed an important role of immunometabolites such as SCFA, butyrate, and amino acids. Conclusions: Our findings showed specific bacterial signature hallmarks of dysbiosis and could represent bacterial biomarkers in IPAA patients useful to develop novel treatments in the future by modulating the gut microbiota through the administration of probiotic immunometabolites-producing bacterial strains and the addition of specific prebiotics and the faecal microbiota transplantation.
format	article
author	Orazio Palmieri Stefano Castellana Giuseppe Biscaglia Anna Panza Anna Latiano Rosanna Fontana Maria Guerra Giuseppe Corritore Tiziana Latiano Giuseppina Martino Tommaso Mazza Angelo Andriulli Francesco Perri Fabrizio Bossa
author_facet	Orazio Palmieri Stefano Castellana Giuseppe Biscaglia Anna Panza Anna Latiano Rosanna Fontana Maria Guerra Giuseppe Corritore Tiziana Latiano Giuseppina Martino Tommaso Mazza Angelo Andriulli Francesco Perri Fabrizio Bossa
author_sort	Orazio Palmieri
title	Microbiome Analysis of Mucosal Ileoanal Pouch in Ulcerative Colitis Patients Revealed Impairment of the Pouches Immunometabolites
title_short	Microbiome Analysis of Mucosal Ileoanal Pouch in Ulcerative Colitis Patients Revealed Impairment of the Pouches Immunometabolites
title_full	Microbiome Analysis of Mucosal Ileoanal Pouch in Ulcerative Colitis Patients Revealed Impairment of the Pouches Immunometabolites
title_fullStr	Microbiome Analysis of Mucosal Ileoanal Pouch in Ulcerative Colitis Patients Revealed Impairment of the Pouches Immunometabolites
title_full_unstemmed	Microbiome Analysis of Mucosal Ileoanal Pouch in Ulcerative Colitis Patients Revealed Impairment of the Pouches Immunometabolites
title_sort	microbiome analysis of mucosal ileoanal pouch in ulcerative colitis patients revealed impairment of the pouches immunometabolites
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9dbf4f34c366414090a026ca87cc603f
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Microbiome Analysis of Mucosal Ileoanal Pouch in Ulcerative Colitis Patients Revealed Impairment of the Pouches Immunometabolites

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