Target identification of small molecules using large-scale CRISPR-Cas mutagenesis scanning of essential genes

Target identification of small molecules using large-scale CRISPR-Cas mutagenesis scanning of essential genes

Cancer therapy drugs are designed to target genetic vulnerabilities, but loss-of-function screens often fail to identify essential genes in drug mechanism studies. Here the authors demonstrate CRISPRres, which exploits in-frame variation generated by indel formation to discover gene-drug interaction...

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Autores principales: Jasper Edgar Neggers, Bert Kwanten, Tim Dierckx, Hiroki Noguchi, Arnout Voet, Lotte Bral, Kristien Minner, Bob Massant, Nicolas Kint, Michel Delforge, Thomas Vercruysse, Erkan Baloglu, William Senapedis, Maarten Jacquemyn, Dirk Daelemans
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Q
Acceso en línea:https://doaj.org/article/9dbf9e90c64d440ea8f766544059bc01
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spelling oai:doaj.org-article:9dbf9e90c64d440ea8f766544059bc012021-12-02T14:39:09ZTarget identification of small molecules using large-scale CRISPR-Cas mutagenesis scanning of essential genes10.1038/s41467-017-02349-82041-1723https://doaj.org/article/9dbf9e90c64d440ea8f766544059bc012018-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-02349-8https://doaj.org/toc/2041-1723Cancer therapy drugs are designed to target genetic vulnerabilities, but loss-of-function screens often fail to identify essential genes in drug mechanism studies. Here the authors demonstrate CRISPRres, which exploits in-frame variation generated by indel formation to discover gene-drug interactions.Jasper Edgar NeggersBert KwantenTim DierckxHiroki NoguchiArnout VoetLotte BralKristien MinnerBob MassantNicolas KintMichel DelforgeThomas VercruysseErkan BalogluWilliam SenapedisMaarten JacquemynDirk DaelemansNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-14 (2018)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Jasper Edgar Neggers
Bert Kwanten
Tim Dierckx
Hiroki Noguchi
Arnout Voet
Lotte Bral
Kristien Minner
Bob Massant
Nicolas Kint
Michel Delforge
Thomas Vercruysse
Erkan Baloglu
William Senapedis
Maarten Jacquemyn
Dirk Daelemans
Target identification of small molecules using large-scale CRISPR-Cas mutagenesis scanning of essential genes
description Cancer therapy drugs are designed to target genetic vulnerabilities, but loss-of-function screens often fail to identify essential genes in drug mechanism studies. Here the authors demonstrate CRISPRres, which exploits in-frame variation generated by indel formation to discover gene-drug interactions.
format article
author Jasper Edgar Neggers
Bert Kwanten
Tim Dierckx
Hiroki Noguchi
Arnout Voet
Lotte Bral
Kristien Minner
Bob Massant
Nicolas Kint
Michel Delforge
Thomas Vercruysse
Erkan Baloglu
William Senapedis
Maarten Jacquemyn
Dirk Daelemans
author_facet Jasper Edgar Neggers
Bert Kwanten
Tim Dierckx
Hiroki Noguchi
Arnout Voet
Lotte Bral
Kristien Minner
Bob Massant
Nicolas Kint
Michel Delforge
Thomas Vercruysse
Erkan Baloglu
William Senapedis
Maarten Jacquemyn
Dirk Daelemans
author_sort Jasper Edgar Neggers
title Target identification of small molecules using large-scale CRISPR-Cas mutagenesis scanning of essential genes
title_short Target identification of small molecules using large-scale CRISPR-Cas mutagenesis scanning of essential genes
title_full Target identification of small molecules using large-scale CRISPR-Cas mutagenesis scanning of essential genes
title_fullStr Target identification of small molecules using large-scale CRISPR-Cas mutagenesis scanning of essential genes
title_full_unstemmed Target identification of small molecules using large-scale CRISPR-Cas mutagenesis scanning of essential genes
title_sort target identification of small molecules using large-scale crispr-cas mutagenesis scanning of essential genes
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/9dbf9e90c64d440ea8f766544059bc01
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