Identification of Drought Tolerant and Molecular Analysis of DREB2A and BADH2 Genes and Yield Potensial of Lines from Single Crossing Bengkulu Local Rice Varieties

Screening in the seedling stage of 39 progeny of F6 lines to drought stress was carried out in the greenhouse. Drought tolerant and sensitive varieties of IR 20 and Salumpikit, respectively, were used as control plants. The methods for traits identification of leaf curled, dried, and recovery abil...

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Autores principales:	Reny Herawati, ALNOPRI ALNOPRI, MASDAR MASDAR, MARULAK SIMARMATA, SIPRIYADI SIPRIYADI, MIMI SUTRAWATI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	badh2, dreb2a, drought tolerance, gene identification, yield potential Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9ddaf58103014ebc96855dafc715914c
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spelling	oai:doaj.org-article:9ddaf58103014ebc96855dafc715914c2021-11-22T00:54:56ZIdentification of Drought Tolerant and Molecular Analysis of DREB2A and BADH2 Genes and Yield Potensial of Lines from Single Crossing Bengkulu Local Rice Varieties1412-033X2085-472210.13057/biodiv/d220232https://doaj.org/article/9ddaf58103014ebc96855dafc715914c2021-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6884https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Screening in the seedling stage of 39 progeny of F6 lines to drought stress was carried out in the greenhouse. Drought tolerant and sensitive varieties of IR 20 and Salumpikit, respectively, were used as control plants. The methods for traits identification of leaf curled, dried, and recovery ability after exposure to severe drought for two weeks was following the Standard Evaluation System (SES) developed by IRRI. Molecular analysis to detect the presence of the DREB2A gene was carried out by PCR amplification of genomic DNA using forward- and reverse- oligonucleotide primers of CCTCATTGGGTCAGGAAGAA and GGATCTCAGCCACCCACTTA, respectively, while for BADH2 gene using forward- and reverse- oligonucleotide primers of GGCCAAGTACCTCAAGGCGA and TGTCCCCAGCTGCTTCATCC, respectively. Molecular markers of DREB2A and BADH2 genes were also identified in 39 tested lines with approximately 250 and 2300 bp length, respectively. This study concluded that the progeny of F6 lines generating from the crossing of local varieties of IR7858 and IR148 is the potential to become a drought-tolerant variety of upland rice. Line numbers BKL2 B-2-264-6 and BKL4 B-1-268-10 have a potential yield of more than 12 tonnes/ha. These line has the potential to be developed on rainfed lowland rice or dry land because it has drought resistance.Reny HerawatiALNOPRI ALNOPRIMASDAR MASDARMARULAK SIMARMATASIPRIYADI SIPRIYADIMIMI SUTRAWATIMBI & UNS Soloarticlebadh2, dreb2a, drought tolerance, gene identification, yield potentialBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	badh2, dreb2a, drought tolerance, gene identification, yield potential Biology (General) QH301-705.5
spellingShingle	badh2, dreb2a, drought tolerance, gene identification, yield potential Biology (General) QH301-705.5 Reny Herawati ALNOPRI ALNOPRI MASDAR MASDAR MARULAK SIMARMATA SIPRIYADI SIPRIYADI MIMI SUTRAWATI Identification of Drought Tolerant and Molecular Analysis of DREB2A and BADH2 Genes and Yield Potensial of Lines from Single Crossing Bengkulu Local Rice Varieties
description	Screening in the seedling stage of 39 progeny of F6 lines to drought stress was carried out in the greenhouse. Drought tolerant and sensitive varieties of IR 20 and Salumpikit, respectively, were used as control plants. The methods for traits identification of leaf curled, dried, and recovery ability after exposure to severe drought for two weeks was following the Standard Evaluation System (SES) developed by IRRI. Molecular analysis to detect the presence of the DREB2A gene was carried out by PCR amplification of genomic DNA using forward- and reverse- oligonucleotide primers of CCTCATTGGGTCAGGAAGAA and GGATCTCAGCCACCCACTTA, respectively, while for BADH2 gene using forward- and reverse- oligonucleotide primers of GGCCAAGTACCTCAAGGCGA and TGTCCCCAGCTGCTTCATCC, respectively. Molecular markers of DREB2A and BADH2 genes were also identified in 39 tested lines with approximately 250 and 2300 bp length, respectively. This study concluded that the progeny of F6 lines generating from the crossing of local varieties of IR7858 and IR148 is the potential to become a drought-tolerant variety of upland rice. Line numbers BKL2 B-2-264-6 and BKL4 B-1-268-10 have a potential yield of more than 12 tonnes/ha. These line has the potential to be developed on rainfed lowland rice or dry land because it has drought resistance.
format	article
author	Reny Herawati ALNOPRI ALNOPRI MASDAR MASDAR MARULAK SIMARMATA SIPRIYADI SIPRIYADI MIMI SUTRAWATI
author_facet	Reny Herawati ALNOPRI ALNOPRI MASDAR MASDAR MARULAK SIMARMATA SIPRIYADI SIPRIYADI MIMI SUTRAWATI
author_sort	Reny Herawati
title	Identification of Drought Tolerant and Molecular Analysis of DREB2A and BADH2 Genes and Yield Potensial of Lines from Single Crossing Bengkulu Local Rice Varieties
title_short	Identification of Drought Tolerant and Molecular Analysis of DREB2A and BADH2 Genes and Yield Potensial of Lines from Single Crossing Bengkulu Local Rice Varieties
title_full	Identification of Drought Tolerant and Molecular Analysis of DREB2A and BADH2 Genes and Yield Potensial of Lines from Single Crossing Bengkulu Local Rice Varieties
title_fullStr	Identification of Drought Tolerant and Molecular Analysis of DREB2A and BADH2 Genes and Yield Potensial of Lines from Single Crossing Bengkulu Local Rice Varieties
title_full_unstemmed	Identification of Drought Tolerant and Molecular Analysis of DREB2A and BADH2 Genes and Yield Potensial of Lines from Single Crossing Bengkulu Local Rice Varieties
title_sort	identification of drought tolerant and molecular analysis of dreb2a and badh2 genes and yield potensial of lines from single crossing bengkulu local rice varieties
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/9ddaf58103014ebc96855dafc715914c
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Identification of Drought Tolerant and Molecular Analysis of DREB2A and BADH2 Genes and Yield Potensial of Lines from Single Crossing Bengkulu Local Rice Varieties

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