Chitosan Enhances the Anti-Biofilm Activity of Biodentine against an Interkingdom Biofilm Model

Endodontic infection is a biofilm disease that is difficult to irradicate with current treatment protocols, and as such, persistent micro-organisms may lead to ongoing or recurrent disease. The potential for the use of enhanced filling materials to modify biofilm regrowth is a promising strategy. Th...

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Autores principales:	Sumaya Abusrewil, Jason L. Brown, Christopher Delaney, Mark C. Butcher, Mohammed Tiba, J. Alun Scott, Gordon Ramage, William McLean
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	endodontics bioceramics chitosan biofilms antimicrobials interkingdom interactions Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/9de25872b487433fb2c59b2e733b47f8
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spelling	oai:doaj.org-article:9de25872b487433fb2c59b2e733b47f82021-11-25T16:22:40ZChitosan Enhances the Anti-Biofilm Activity of Biodentine against an Interkingdom Biofilm Model10.3390/antibiotics101113172079-6382https://doaj.org/article/9de25872b487433fb2c59b2e733b47f82021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1317https://doaj.org/toc/2079-6382Endodontic infection is a biofilm disease that is difficult to irradicate with current treatment protocols, and as such, persistent micro-organisms may lead to ongoing or recurrent disease. The potential for the use of enhanced filling materials to modify biofilm regrowth is a promising strategy. This current study aimed to evaluate the anti-biofilm efficacy of calcium silicate cements modified with chitosan. The development of mono-species and multi-species biofilms on ProRoot MTA, Biodentine and bovine dentine discs were explored using quantitative microbiology analysis. The effect on regrowth of biofilms was assessed following the addition of chitosan to each cement. In comparison to a dentine substrate, both materials did not show the ability to inhibit biofilm regrowth. Biodentine incorporated with chitosan displayed a dose-dependent reduction in multi-species biofilm regrowth, unlike MTA. Notably, interkingdom biofilms were shown to enhance bacterial tolerance in the presence of chitosan. This study demonstrates the potential to enhance the antimicrobial properties of Biodentine. The findings highlight the need for appropriate model systems when exploring antimicrobial properties of materials in vitro so that interspecies and interkingdom interactions that modify tolerance are not overlooked while still supporting the development of innovative materials.Sumaya AbusrewilJason L. BrownChristopher DelaneyMark C. ButcherMohammed TibaJ. Alun ScottGordon RamageWilliam McLeanMDPI AGarticleendodonticsbioceramicschitosanbiofilmsantimicrobialsinterkingdom interactionsTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1317, p 1317 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endodontics bioceramics chitosan biofilms antimicrobials interkingdom interactions Therapeutics. Pharmacology RM1-950
spellingShingle	endodontics bioceramics chitosan biofilms antimicrobials interkingdom interactions Therapeutics. Pharmacology RM1-950 Sumaya Abusrewil Jason L. Brown Christopher Delaney Mark C. Butcher Mohammed Tiba J. Alun Scott Gordon Ramage William McLean Chitosan Enhances the Anti-Biofilm Activity of Biodentine against an Interkingdom Biofilm Model
description	Endodontic infection is a biofilm disease that is difficult to irradicate with current treatment protocols, and as such, persistent micro-organisms may lead to ongoing or recurrent disease. The potential for the use of enhanced filling materials to modify biofilm regrowth is a promising strategy. This current study aimed to evaluate the anti-biofilm efficacy of calcium silicate cements modified with chitosan. The development of mono-species and multi-species biofilms on ProRoot MTA, Biodentine and bovine dentine discs were explored using quantitative microbiology analysis. The effect on regrowth of biofilms was assessed following the addition of chitosan to each cement. In comparison to a dentine substrate, both materials did not show the ability to inhibit biofilm regrowth. Biodentine incorporated with chitosan displayed a dose-dependent reduction in multi-species biofilm regrowth, unlike MTA. Notably, interkingdom biofilms were shown to enhance bacterial tolerance in the presence of chitosan. This study demonstrates the potential to enhance the antimicrobial properties of Biodentine. The findings highlight the need for appropriate model systems when exploring antimicrobial properties of materials in vitro so that interspecies and interkingdom interactions that modify tolerance are not overlooked while still supporting the development of innovative materials.
format	article
author	Sumaya Abusrewil Jason L. Brown Christopher Delaney Mark C. Butcher Mohammed Tiba J. Alun Scott Gordon Ramage William McLean
author_facet	Sumaya Abusrewil Jason L. Brown Christopher Delaney Mark C. Butcher Mohammed Tiba J. Alun Scott Gordon Ramage William McLean
author_sort	Sumaya Abusrewil
title	Chitosan Enhances the Anti-Biofilm Activity of Biodentine against an Interkingdom Biofilm Model
title_short	Chitosan Enhances the Anti-Biofilm Activity of Biodentine against an Interkingdom Biofilm Model
title_full	Chitosan Enhances the Anti-Biofilm Activity of Biodentine against an Interkingdom Biofilm Model
title_fullStr	Chitosan Enhances the Anti-Biofilm Activity of Biodentine against an Interkingdom Biofilm Model
title_full_unstemmed	Chitosan Enhances the Anti-Biofilm Activity of Biodentine against an Interkingdom Biofilm Model
title_sort	chitosan enhances the anti-biofilm activity of biodentine against an interkingdom biofilm model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9de25872b487433fb2c59b2e733b47f8
work_keys_str_mv	AT sumayaabusrewil chitosanenhancestheantibiofilmactivityofbiodentineagainstaninterkingdombiofilmmodel AT jasonlbrown chitosanenhancestheantibiofilmactivityofbiodentineagainstaninterkingdombiofilmmodel AT christopherdelaney chitosanenhancestheantibiofilmactivityofbiodentineagainstaninterkingdombiofilmmodel AT markcbutcher chitosanenhancestheantibiofilmactivityofbiodentineagainstaninterkingdombiofilmmodel AT mohammedtiba chitosanenhancestheantibiofilmactivityofbiodentineagainstaninterkingdombiofilmmodel AT jalunscott chitosanenhancestheantibiofilmactivityofbiodentineagainstaninterkingdombiofilmmodel AT gordonramage chitosanenhancestheantibiofilmactivityofbiodentineagainstaninterkingdombiofilmmodel AT williammclean chitosanenhancestheantibiofilmactivityofbiodentineagainstaninterkingdombiofilmmodel
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Chitosan Enhances the Anti-Biofilm Activity of Biodentine against an Interkingdom Biofilm Model

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