Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection

Abstract Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging...

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Autores principales:	Annika Schneider, Sandra Kurz, Katrin Manske, Marianne Janas, Mathias Heikenwälder, Thomas Misgeld, Michaela Aichler, Sebastian Felix Weissmann, Hans Zischka, Percy Knolle, Dirk Wohlleber
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9deaae37133b4a689730d1568a261e15
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spelling	oai:doaj.org-article:9deaae37133b4a689730d1568a261e152021-12-02T15:08:58ZSingle organelle analysis to characterize mitochondrial function and crosstalk during viral infection10.1038/s41598-019-44922-92045-2322https://doaj.org/article/9deaae37133b4a689730d1568a261e152019-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-44922-9https://doaj.org/toc/2045-2322Abstract Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.Annika SchneiderSandra KurzKatrin ManskeMarianne JanasMathias HeikenwälderThomas MisgeldMichaela AichlerSebastian Felix WeissmannHans ZischkaPercy KnolleDirk WohlleberNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Annika Schneider Sandra Kurz Katrin Manske Marianne Janas Mathias Heikenwälder Thomas Misgeld Michaela Aichler Sebastian Felix Weissmann Hans Zischka Percy Knolle Dirk Wohlleber Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
description	Abstract Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.
format	article
author	Annika Schneider Sandra Kurz Katrin Manske Marianne Janas Mathias Heikenwälder Thomas Misgeld Michaela Aichler Sebastian Felix Weissmann Hans Zischka Percy Knolle Dirk Wohlleber
author_facet	Annika Schneider Sandra Kurz Katrin Manske Marianne Janas Mathias Heikenwälder Thomas Misgeld Michaela Aichler Sebastian Felix Weissmann Hans Zischka Percy Knolle Dirk Wohlleber
author_sort	Annika Schneider
title	Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
title_short	Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
title_full	Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
title_fullStr	Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
title_full_unstemmed	Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
title_sort	single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/9deaae37133b4a689730d1568a261e15
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Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection

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