Transcriptome and metabolome analyses revealing the potential mechanism of seed germination in Polygonatum cyrtonema

Abstract Polygonatum cyrtonema Hua (Huangjing, HJ) has medicinal and edible value in China. However, the seeds of this plant are naturally difficult to germinate. Therefore, to elucidate the mechanism underlying the germination of this plant in order to meet the market demand, the metabolomic and tr...

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Autores principales:	Rong Liu, Jing Lu, Jiayi Xing, Mei Du, Mingxiu Wang, Lei Zhang, Yunfang Li, Chihong Zhang, Yu Wu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9defa00034c844529d98a60440e11ba4
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spelling	oai:doaj.org-article:9defa00034c844529d98a60440e11ba42021-12-02T17:47:15ZTranscriptome and metabolome analyses revealing the potential mechanism of seed germination in Polygonatum cyrtonema10.1038/s41598-021-91598-12045-2322https://doaj.org/article/9defa00034c844529d98a60440e11ba42021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91598-1https://doaj.org/toc/2045-2322Abstract Polygonatum cyrtonema Hua (Huangjing, HJ) has medicinal and edible value in China. However, the seeds of this plant are naturally difficult to germinate. Therefore, to elucidate the mechanism underlying the germination of this plant in order to meet the market demand, the metabolomic and transcriptomic analyses were performed in this study. We observed that plant hormones and α-amylase activity were differentially regulated when comparing germinated and un-germinated seeds. In addition, the metabolites related to phenylpropanoid and flavonoid biosynthesis were significantly up-accumulated in germinated seeds. Hydroxycinnamoyl derivatives and organic acids were observed to be significantly decreased during germination. The results of this study suggested that compared to un-germinated seeds, germinated seeds promote flavonoid synthesis and inhibit lignin synthesis which could be beneficial to the germination of HJ seeds. Furthermore, these results suggested that starch if hydrolyzed into glucose, which could provide the necessary energy for germination. Our results may help to establish a foundation for further research investigating the regulatory networks of seed germination and may facilitate the propagation of HJ seeds.Rong LiuJing LuJiayi XingMei DuMingxiu WangLei ZhangYunfang LiChihong ZhangYu WuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rong Liu Jing Lu Jiayi Xing Mei Du Mingxiu Wang Lei Zhang Yunfang Li Chihong Zhang Yu Wu Transcriptome and metabolome analyses revealing the potential mechanism of seed germination in Polygonatum cyrtonema
description	Abstract Polygonatum cyrtonema Hua (Huangjing, HJ) has medicinal and edible value in China. However, the seeds of this plant are naturally difficult to germinate. Therefore, to elucidate the mechanism underlying the germination of this plant in order to meet the market demand, the metabolomic and transcriptomic analyses were performed in this study. We observed that plant hormones and α-amylase activity were differentially regulated when comparing germinated and un-germinated seeds. In addition, the metabolites related to phenylpropanoid and flavonoid biosynthesis were significantly up-accumulated in germinated seeds. Hydroxycinnamoyl derivatives and organic acids were observed to be significantly decreased during germination. The results of this study suggested that compared to un-germinated seeds, germinated seeds promote flavonoid synthesis and inhibit lignin synthesis which could be beneficial to the germination of HJ seeds. Furthermore, these results suggested that starch if hydrolyzed into glucose, which could provide the necessary energy for germination. Our results may help to establish a foundation for further research investigating the regulatory networks of seed germination and may facilitate the propagation of HJ seeds.
format	article
author	Rong Liu Jing Lu Jiayi Xing Mei Du Mingxiu Wang Lei Zhang Yunfang Li Chihong Zhang Yu Wu
author_facet	Rong Liu Jing Lu Jiayi Xing Mei Du Mingxiu Wang Lei Zhang Yunfang Li Chihong Zhang Yu Wu
author_sort	Rong Liu
title	Transcriptome and metabolome analyses revealing the potential mechanism of seed germination in Polygonatum cyrtonema
title_short	Transcriptome and metabolome analyses revealing the potential mechanism of seed germination in Polygonatum cyrtonema
title_full	Transcriptome and metabolome analyses revealing the potential mechanism of seed germination in Polygonatum cyrtonema
title_fullStr	Transcriptome and metabolome analyses revealing the potential mechanism of seed germination in Polygonatum cyrtonema
title_full_unstemmed	Transcriptome and metabolome analyses revealing the potential mechanism of seed germination in Polygonatum cyrtonema
title_sort	transcriptome and metabolome analyses revealing the potential mechanism of seed germination in polygonatum cyrtonema
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9defa00034c844529d98a60440e11ba4
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Transcriptome and metabolome analyses revealing the potential mechanism of seed germination in Polygonatum cyrtonema

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