Root Suberin Plays Important Roles in Reducing Water Loss and Sodium Uptake in <i>Arabidopsis thaliana</i>

Suberin is a cell-wall-associated hetero-polymer deposited in specific plant tissues. The precise role of its composition and lamellae structure in protecting plants against abiotic stresses is unclear. In <i>Arabidopsis thaliana</i>, we tested the biochemical and physiological responses...

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Autores principales:	Nayana D. G. de Silva, Jhadeswar Murmu, Denise Chabot, Keith Hubbard, Peter Ryser, Isabel Molina, Owen Rowland
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	suberin waxes chronic drought salt stress root periderm K/Na ratio Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9df0ea21062c4cd38b7a7c85ad5de195
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spelling	oai:doaj.org-article:9df0ea21062c4cd38b7a7c85ad5de1952021-11-25T18:20:32ZRoot Suberin Plays Important Roles in Reducing Water Loss and Sodium Uptake in <i>Arabidopsis thaliana</i>10.3390/metabo111107352218-1989https://doaj.org/article/9df0ea21062c4cd38b7a7c85ad5de1952021-10-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/735https://doaj.org/toc/2218-1989Suberin is a cell-wall-associated hetero-polymer deposited in specific plant tissues. The precise role of its composition and lamellae structure in protecting plants against abiotic stresses is unclear. In <i>Arabidopsis thaliana</i>, we tested the biochemical and physiological responses to water deficiency and NaCl treatment in mutants that are differentially affected in suberin composition and lamellae structure. Chronic drought stress increased suberin and suberin-associated waxes in wild-type plants. Suberin-deficient mutants were not more susceptible than the wild-type to the chronic drought stress imposed in this study. Nonetheless, the <i>cyp86a1-1 cyp86b1-1</i> mutant, which had a severely altered suberin composition and lamellae structure, exhibited increased water loss through the root periderm. <i>Cyp86a1-1 cyp86b1-1</i> also recorded lower relative water content in leaves. The <i>abcg2-1 abcg6-1 abcg20-1</i> mutant, which has altered suberin composition and lamellae, was very sensitive to NaCl treatment. Furthermore, <i>cyp86a1-1 cyp86b1-1</i> recorded a significant drop in the leaf K/Na ratio, indicating salt sensitivity. The <i>far1-2 far4-1 far5-1</i> mutant, which did not show structural defects in the suberin lamellae, had similar responses to drought and NaCl treatments as the wild-type. Our results provide evidence that the suberin amount and lamellae structure are key features in the barrier function of suberin in reducing water loss and reducing sodium uptake through roots for better performance under drought and salt stresses.Nayana D. G. de SilvaJhadeswar MurmuDenise ChabotKeith HubbardPeter RyserIsabel MolinaOwen RowlandMDPI AGarticlesuberinwaxeschronic droughtsalt stressroot peridermK/Na ratioMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 735, p 735 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	suberin waxes chronic drought salt stress root periderm K/Na ratio Microbiology QR1-502
spellingShingle	suberin waxes chronic drought salt stress root periderm K/Na ratio Microbiology QR1-502 Nayana D. G. de Silva Jhadeswar Murmu Denise Chabot Keith Hubbard Peter Ryser Isabel Molina Owen Rowland Root Suberin Plays Important Roles in Reducing Water Loss and Sodium Uptake in <i>Arabidopsis thaliana</i>
description	Suberin is a cell-wall-associated hetero-polymer deposited in specific plant tissues. The precise role of its composition and lamellae structure in protecting plants against abiotic stresses is unclear. In <i>Arabidopsis thaliana</i>, we tested the biochemical and physiological responses to water deficiency and NaCl treatment in mutants that are differentially affected in suberin composition and lamellae structure. Chronic drought stress increased suberin and suberin-associated waxes in wild-type plants. Suberin-deficient mutants were not more susceptible than the wild-type to the chronic drought stress imposed in this study. Nonetheless, the <i>cyp86a1-1 cyp86b1-1</i> mutant, which had a severely altered suberin composition and lamellae structure, exhibited increased water loss through the root periderm. <i>Cyp86a1-1 cyp86b1-1</i> also recorded lower relative water content in leaves. The <i>abcg2-1 abcg6-1 abcg20-1</i> mutant, which has altered suberin composition and lamellae, was very sensitive to NaCl treatment. Furthermore, <i>cyp86a1-1 cyp86b1-1</i> recorded a significant drop in the leaf K/Na ratio, indicating salt sensitivity. The <i>far1-2 far4-1 far5-1</i> mutant, which did not show structural defects in the suberin lamellae, had similar responses to drought and NaCl treatments as the wild-type. Our results provide evidence that the suberin amount and lamellae structure are key features in the barrier function of suberin in reducing water loss and reducing sodium uptake through roots for better performance under drought and salt stresses.
format	article
author	Nayana D. G. de Silva Jhadeswar Murmu Denise Chabot Keith Hubbard Peter Ryser Isabel Molina Owen Rowland
author_facet	Nayana D. G. de Silva Jhadeswar Murmu Denise Chabot Keith Hubbard Peter Ryser Isabel Molina Owen Rowland
author_sort	Nayana D. G. de Silva
title	Root Suberin Plays Important Roles in Reducing Water Loss and Sodium Uptake in <i>Arabidopsis thaliana</i>
title_short	Root Suberin Plays Important Roles in Reducing Water Loss and Sodium Uptake in <i>Arabidopsis thaliana</i>
title_full	Root Suberin Plays Important Roles in Reducing Water Loss and Sodium Uptake in <i>Arabidopsis thaliana</i>
title_fullStr	Root Suberin Plays Important Roles in Reducing Water Loss and Sodium Uptake in <i>Arabidopsis thaliana</i>
title_full_unstemmed	Root Suberin Plays Important Roles in Reducing Water Loss and Sodium Uptake in <i>Arabidopsis thaliana</i>
title_sort	root suberin plays important roles in reducing water loss and sodium uptake in <i>arabidopsis thaliana</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9df0ea21062c4cd38b7a7c85ad5de195
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Root Suberin Plays Important Roles in Reducing Water Loss and Sodium Uptake in <i>Arabidopsis thaliana</i>

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