Biomarker discovery in subclinical mycobacterial infections of cattle.

<h4>Background</h4>Bovine tuberculosis is a highly prevalent infectious disease of cattle worldwide; however, infection in the United States is limited to 0.01% of dairy herds. Thus detection of bovine TB is confounded by high background infection with M. avium subsp. paratuberculosis. T...

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Autores principales:	Meetu Seth, Elise A Lamont, Harish K Janagama, Andrea Widdel, Lucy Vulchanova, Judith R Stabel, W Ray Waters, Mitchell V Palmer, Srinand Sreevatsan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9df1809fcacb47eebc44d776f2c59d77
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spelling	oai:doaj.org-article:9df1809fcacb47eebc44d776f2c59d772021-11-25T06:22:47ZBiomarker discovery in subclinical mycobacterial infections of cattle.1932-620310.1371/journal.pone.0005478https://doaj.org/article/9df1809fcacb47eebc44d776f2c59d772009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19424492/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Bovine tuberculosis is a highly prevalent infectious disease of cattle worldwide; however, infection in the United States is limited to 0.01% of dairy herds. Thus detection of bovine TB is confounded by high background infection with M. avium subsp. paratuberculosis. The present study addresses variations in the circulating peptidome based on the pathogenesis of two biologically similar mycobacterial diseases of cattle.<h4>Methodology/principal findings</h4>We hypothesized that serum proteomes of animals in response to either M. bovis or M. paratuberculosis infection will display several commonalities and differences. Sera prospectively collected from animals experimentally infected with either M. bovis or M. paratuberculosis were analyzed using high-resolution proteomics approaches. iTRAQ, a liquid chromatography and tandem mass spectrometry approach, was used to simultaneously identify and quantify peptides from multiple infections and contemporaneous uninfected control groups. Four comparisons were performed: 1) M. bovis infection versus uninfected controls, 2) M. bovis versus M. paratuberculosis infection, 3) early, and 4) advanced M. paratuberculosis infection versus uninfected controls. One hundred and ten differentially elevated proteins (P < or = 0.05) were identified. Vitamin D binding protein precursor (DBP), alpha-1 acid glycoprotein, alpha-1B glycoprotein, fetuin, and serine proteinase inhibitor were identified in both infections. Transthyretin, retinol binding proteins, and cathelicidin were identified exclusively in M. paratuberculosis infection, while the serum levels of alpha-1-microglobulin/bikunin precursor (AMBP) protein, alpha-1 acid glycoprotein, fetuin, and alpha-1B glycoprotein were elevated exclusively in M. bovis infected animals.<h4>Conclusions/significance</h4>The discovery of these biomarkers has significant impact on the elucidation of pathogenesis of two mycobacterial diseases at the cellular and the molecular level and can be applied in the development of mycobacterium-specific diagnostic tools for the monitoring progression of disease, response to therapy, and/or vaccine based interventions.Meetu SethElise A LamontHarish K JanagamaAndrea WiddelLucy VulchanovaJudith R StabelW Ray WatersMitchell V PalmerSrinand SreevatsanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 5, p e5478 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meetu Seth Elise A Lamont Harish K Janagama Andrea Widdel Lucy Vulchanova Judith R Stabel W Ray Waters Mitchell V Palmer Srinand Sreevatsan Biomarker discovery in subclinical mycobacterial infections of cattle.
description	<h4>Background</h4>Bovine tuberculosis is a highly prevalent infectious disease of cattle worldwide; however, infection in the United States is limited to 0.01% of dairy herds. Thus detection of bovine TB is confounded by high background infection with M. avium subsp. paratuberculosis. The present study addresses variations in the circulating peptidome based on the pathogenesis of two biologically similar mycobacterial diseases of cattle.<h4>Methodology/principal findings</h4>We hypothesized that serum proteomes of animals in response to either M. bovis or M. paratuberculosis infection will display several commonalities and differences. Sera prospectively collected from animals experimentally infected with either M. bovis or M. paratuberculosis were analyzed using high-resolution proteomics approaches. iTRAQ, a liquid chromatography and tandem mass spectrometry approach, was used to simultaneously identify and quantify peptides from multiple infections and contemporaneous uninfected control groups. Four comparisons were performed: 1) M. bovis infection versus uninfected controls, 2) M. bovis versus M. paratuberculosis infection, 3) early, and 4) advanced M. paratuberculosis infection versus uninfected controls. One hundred and ten differentially elevated proteins (P < or = 0.05) were identified. Vitamin D binding protein precursor (DBP), alpha-1 acid glycoprotein, alpha-1B glycoprotein, fetuin, and serine proteinase inhibitor were identified in both infections. Transthyretin, retinol binding proteins, and cathelicidin were identified exclusively in M. paratuberculosis infection, while the serum levels of alpha-1-microglobulin/bikunin precursor (AMBP) protein, alpha-1 acid glycoprotein, fetuin, and alpha-1B glycoprotein were elevated exclusively in M. bovis infected animals.<h4>Conclusions/significance</h4>The discovery of these biomarkers has significant impact on the elucidation of pathogenesis of two mycobacterial diseases at the cellular and the molecular level and can be applied in the development of mycobacterium-specific diagnostic tools for the monitoring progression of disease, response to therapy, and/or vaccine based interventions.
format	article
author	Meetu Seth Elise A Lamont Harish K Janagama Andrea Widdel Lucy Vulchanova Judith R Stabel W Ray Waters Mitchell V Palmer Srinand Sreevatsan
author_facet	Meetu Seth Elise A Lamont Harish K Janagama Andrea Widdel Lucy Vulchanova Judith R Stabel W Ray Waters Mitchell V Palmer Srinand Sreevatsan
author_sort	Meetu Seth
title	Biomarker discovery in subclinical mycobacterial infections of cattle.
title_short	Biomarker discovery in subclinical mycobacterial infections of cattle.
title_full	Biomarker discovery in subclinical mycobacterial infections of cattle.
title_fullStr	Biomarker discovery in subclinical mycobacterial infections of cattle.
title_full_unstemmed	Biomarker discovery in subclinical mycobacterial infections of cattle.
title_sort	biomarker discovery in subclinical mycobacterial infections of cattle.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/9df1809fcacb47eebc44d776f2c59d77
work_keys_str_mv	AT meetuseth biomarkerdiscoveryinsubclinicalmycobacterialinfectionsofcattle AT elisealamont biomarkerdiscoveryinsubclinicalmycobacterialinfectionsofcattle AT harishkjanagama biomarkerdiscoveryinsubclinicalmycobacterialinfectionsofcattle AT andreawiddel biomarkerdiscoveryinsubclinicalmycobacterialinfectionsofcattle AT lucyvulchanova biomarkerdiscoveryinsubclinicalmycobacterialinfectionsofcattle AT judithrstabel biomarkerdiscoveryinsubclinicalmycobacterialinfectionsofcattle AT wraywaters biomarkerdiscoveryinsubclinicalmycobacterialinfectionsofcattle AT mitchellvpalmer biomarkerdiscoveryinsubclinicalmycobacterialinfectionsofcattle AT srinandsreevatsan biomarkerdiscoveryinsubclinicalmycobacterialinfectionsofcattle
_version_	1718413816747786240

Biomarker discovery in subclinical mycobacterial infections of cattle.

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