Carbohydrate availability regulates virulence gene expression in Streptococcus suis.

Streptococcus suis is a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry. S. suis is also an emerging pathogen of humans. Colonization of porcine oropharynx by S. suis is considered to be a high risk factor for invasive disease. In the oropharyngeal cav...

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Autores principales:	M Laura Ferrando, Peter van Baarlen, Germano Orrù, Rosaria Piga, Roger S Bongers, Michiel Wels, Astrid De Greeff, Hilde E Smith, Jerry M Wells
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9df69f296fb5495c833bccb87d6b7d28
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spelling	oai:doaj.org-article:9df69f296fb5495c833bccb87d6b7d282021-11-18T08:27:33ZCarbohydrate availability regulates virulence gene expression in Streptococcus suis.1932-620310.1371/journal.pone.0089334https://doaj.org/article/9df69f296fb5495c833bccb87d6b7d282014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24642967/?tool=EBIhttps://doaj.org/toc/1932-6203Streptococcus suis is a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry. S. suis is also an emerging pathogen of humans. Colonization of porcine oropharynx by S. suis is considered to be a high risk factor for invasive disease. In the oropharyngeal cavity, where glucose is rapidly absorbed but dietary α-glucans persist, there is a profound effect of carbohydrate availability on the expression of virulence genes. Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source. Additionally the production of suilysin, a toxin that damages epithelial cells, was increased more than ten-fold when glucose levels were low and S. suis was growing on pullulan. Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood. This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.M Laura FerrandoPeter van BaarlenGermano OrrùRosaria PigaRoger S BongersMichiel WelsAstrid De GreeffHilde E SmithJerry M WellsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e89334 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M Laura Ferrando Peter van Baarlen Germano Orrù Rosaria Piga Roger S Bongers Michiel Wels Astrid De Greeff Hilde E Smith Jerry M Wells Carbohydrate availability regulates virulence gene expression in Streptococcus suis.
description	Streptococcus suis is a major bacterial pathogen of young pigs causing worldwide economic problems for the pig industry. S. suis is also an emerging pathogen of humans. Colonization of porcine oropharynx by S. suis is considered to be a high risk factor for invasive disease. In the oropharyngeal cavity, where glucose is rapidly absorbed but dietary α-glucans persist, there is a profound effect of carbohydrate availability on the expression of virulence genes. Nineteen predicted or confirmed S. suis virulence genes that promote adhesion to and invasion of epithelial cells were expressed at higher levels when S. suis was supplied with the α-glucan starch/pullulan compared to glucose as the single carbon source. Additionally the production of suilysin, a toxin that damages epithelial cells, was increased more than ten-fold when glucose levels were low and S. suis was growing on pullulan. Based on biochemical, bioinformatics and in vitro and in vivo gene expression studies, we developed a biological model that postulates the effect of carbon catabolite repression on expression of virulence genes in the mucosa, organs and blood. This research increases our understanding of S. suis virulence mechanisms and has important implications for the design of future control strategies including the development of anti-infective strategies by modulating animal feed composition.
format	article
author	M Laura Ferrando Peter van Baarlen Germano Orrù Rosaria Piga Roger S Bongers Michiel Wels Astrid De Greeff Hilde E Smith Jerry M Wells
author_facet	M Laura Ferrando Peter van Baarlen Germano Orrù Rosaria Piga Roger S Bongers Michiel Wels Astrid De Greeff Hilde E Smith Jerry M Wells
author_sort	M Laura Ferrando
title	Carbohydrate availability regulates virulence gene expression in Streptococcus suis.
title_short	Carbohydrate availability regulates virulence gene expression in Streptococcus suis.
title_full	Carbohydrate availability regulates virulence gene expression in Streptococcus suis.
title_fullStr	Carbohydrate availability regulates virulence gene expression in Streptococcus suis.
title_full_unstemmed	Carbohydrate availability regulates virulence gene expression in Streptococcus suis.
title_sort	carbohydrate availability regulates virulence gene expression in streptococcus suis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/9df69f296fb5495c833bccb87d6b7d28
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_version_	1718421757312892928

Carbohydrate availability regulates virulence gene expression in Streptococcus suis.

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