Assessing the functional impact of PfRh5 genetic diversity on ex vivo erythrocyte invasion inhibition

Abstract The PfRh5-Basigin ligand–receptor interaction is an essential step in the merozoite invasion process and represents an attractive vaccine target. To reveal genotype–phenotype associations between naturally occurring allelic variants of PfRh5 and invasion inhibition, we performed ex vivo inv...

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Autores principales:	Adam J. Moore, Khadidiatou Mangou, Fatoumata Diallo, Seynabou D. Sene, Mariama N. Pouye, Bacary D. Sadio, Ousmane Faye, Alassane Mbengue, Amy K. Bei
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9dfa5e505d1c455888d29f291b816639
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spelling	oai:doaj.org-article:9dfa5e505d1c455888d29f291b8166392021-12-02T13:24:07ZAssessing the functional impact of PfRh5 genetic diversity on ex vivo erythrocyte invasion inhibition10.1038/s41598-021-81711-92045-2322https://doaj.org/article/9dfa5e505d1c455888d29f291b8166392021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81711-9https://doaj.org/toc/2045-2322Abstract The PfRh5-Basigin ligand–receptor interaction is an essential step in the merozoite invasion process and represents an attractive vaccine target. To reveal genotype–phenotype associations between naturally occurring allelic variants of PfRh5 and invasion inhibition, we performed ex vivo invasion inhibition assays with monoclonal antibodies targeting basigin coupled with PfRh5 next-generation amplicon sequencing. We found dose-dependent inhibition of invasion across all isolates tested, and no statistically significant difference in invasion inhibition for any single nucleotide polymorphisms. This study demonstrates that PfRh5 remains highly conserved and functionally essential, even in a highly endemic setting, supporting continued development as a strain-transcendent malaria vaccine target.Adam J. MooreKhadidiatou MangouFatoumata DialloSeynabou D. SeneMariama N. PouyeBacary D. SadioOusmane FayeAlassane MbengueAmy K. BeiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adam J. Moore Khadidiatou Mangou Fatoumata Diallo Seynabou D. Sene Mariama N. Pouye Bacary D. Sadio Ousmane Faye Alassane Mbengue Amy K. Bei Assessing the functional impact of PfRh5 genetic diversity on ex vivo erythrocyte invasion inhibition
description	Abstract The PfRh5-Basigin ligand–receptor interaction is an essential step in the merozoite invasion process and represents an attractive vaccine target. To reveal genotype–phenotype associations between naturally occurring allelic variants of PfRh5 and invasion inhibition, we performed ex vivo invasion inhibition assays with monoclonal antibodies targeting basigin coupled with PfRh5 next-generation amplicon sequencing. We found dose-dependent inhibition of invasion across all isolates tested, and no statistically significant difference in invasion inhibition for any single nucleotide polymorphisms. This study demonstrates that PfRh5 remains highly conserved and functionally essential, even in a highly endemic setting, supporting continued development as a strain-transcendent malaria vaccine target.
format	article
author	Adam J. Moore Khadidiatou Mangou Fatoumata Diallo Seynabou D. Sene Mariama N. Pouye Bacary D. Sadio Ousmane Faye Alassane Mbengue Amy K. Bei
author_facet	Adam J. Moore Khadidiatou Mangou Fatoumata Diallo Seynabou D. Sene Mariama N. Pouye Bacary D. Sadio Ousmane Faye Alassane Mbengue Amy K. Bei
author_sort	Adam J. Moore
title	Assessing the functional impact of PfRh5 genetic diversity on ex vivo erythrocyte invasion inhibition
title_short	Assessing the functional impact of PfRh5 genetic diversity on ex vivo erythrocyte invasion inhibition
title_full	Assessing the functional impact of PfRh5 genetic diversity on ex vivo erythrocyte invasion inhibition
title_fullStr	Assessing the functional impact of PfRh5 genetic diversity on ex vivo erythrocyte invasion inhibition
title_full_unstemmed	Assessing the functional impact of PfRh5 genetic diversity on ex vivo erythrocyte invasion inhibition
title_sort	assessing the functional impact of pfrh5 genetic diversity on ex vivo erythrocyte invasion inhibition
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9dfa5e505d1c455888d29f291b816639
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Assessing the functional impact of PfRh5 genetic diversity on ex vivo erythrocyte invasion inhibition

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