Synergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification

Abstract Background Starch is one of the most important renewable polysaccharides in nature for production of bio-ethanol. The starch saccharification step facilitates the depolymerization of starch to yield glucose for biofuels production. The filamentous fungus Aspergillus niger (A. niger) is the...

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Autores principales:	Wenzhu Guo, Jianhua Yang, Tianchen Huang, Dandan Liu, Qian Liu, Jingen Li, Wenliang Sun, Xingji Wang, Leilei Zhu, Chaoguang Tian
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Aspergillus niger Glucoamylase α-Amylase Starch saccharification Synergistic effects Fuel TP315-360 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/9e0203fd9f1a4cd1ba2daf92706f0724
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spelling	oai:doaj.org-article:9e0203fd9f1a4cd1ba2daf92706f07242021-11-28T12:22:19ZSynergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification10.1186/s13068-021-02074-x1754-6834https://doaj.org/article/9e0203fd9f1a4cd1ba2daf92706f07242021-11-01T00:00:00Zhttps://doi.org/10.1186/s13068-021-02074-xhttps://doaj.org/toc/1754-6834Abstract Background Starch is one of the most important renewable polysaccharides in nature for production of bio-ethanol. The starch saccharification step facilitates the depolymerization of starch to yield glucose for biofuels production. The filamentous fungus Aspergillus niger (A. niger) is the most used microbial cell factory for production of the commercial glucoamylase. However, the role of each component in glucoamylases cocktail of A. niger O1 for starch saccharification remains unclear except glucoamylase. Results In this study, we identified the key enzymes contributing to the starch saccharification process are glucoamylase, α-amylase and acid α-amylase out of 29 glycoside hydrolases from the 6-day fermentation products of A. niger O1. Through the synergistic study of the multienzymes for the starch saccharification in vitro, we found that increasing the amount of α-amylase by 5-10 times enhanced the efficiency of starch saccharification by 14.2-23.2%. Overexpression of acid α-amylase in strain O1 in vivo increased the total glucoamylase activity of O1 cultures by 15.0%. Conclusions Our study clarifies the synergistic effects among the components of glucoamylases cocktail, and provides an effective approach to optimize the profile of saccharifying enzymes of strain O1 for improving the total glucoamylase activity.Wenzhu GuoJianhua YangTianchen HuangDandan LiuQian LiuJingen LiWenliang SunXingji WangLeilei ZhuChaoguang TianBMCarticleAspergillus nigerGlucoamylaseα-AmylaseStarch saccharificationSynergistic effectsFuelTP315-360BiotechnologyTP248.13-248.65ENBiotechnology for Biofuels, Vol 14, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Aspergillus niger Glucoamylase α-Amylase Starch saccharification Synergistic effects Fuel TP315-360 Biotechnology TP248.13-248.65
spellingShingle	Aspergillus niger Glucoamylase α-Amylase Starch saccharification Synergistic effects Fuel TP315-360 Biotechnology TP248.13-248.65 Wenzhu Guo Jianhua Yang Tianchen Huang Dandan Liu Qian Liu Jingen Li Wenliang Sun Xingji Wang Leilei Zhu Chaoguang Tian Synergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification
description	Abstract Background Starch is one of the most important renewable polysaccharides in nature for production of bio-ethanol. The starch saccharification step facilitates the depolymerization of starch to yield glucose for biofuels production. The filamentous fungus Aspergillus niger (A. niger) is the most used microbial cell factory for production of the commercial glucoamylase. However, the role of each component in glucoamylases cocktail of A. niger O1 for starch saccharification remains unclear except glucoamylase. Results In this study, we identified the key enzymes contributing to the starch saccharification process are glucoamylase, α-amylase and acid α-amylase out of 29 glycoside hydrolases from the 6-day fermentation products of A. niger O1. Through the synergistic study of the multienzymes for the starch saccharification in vitro, we found that increasing the amount of α-amylase by 5-10 times enhanced the efficiency of starch saccharification by 14.2-23.2%. Overexpression of acid α-amylase in strain O1 in vivo increased the total glucoamylase activity of O1 cultures by 15.0%. Conclusions Our study clarifies the synergistic effects among the components of glucoamylases cocktail, and provides an effective approach to optimize the profile of saccharifying enzymes of strain O1 for improving the total glucoamylase activity.
format	article
author	Wenzhu Guo Jianhua Yang Tianchen Huang Dandan Liu Qian Liu Jingen Li Wenliang Sun Xingji Wang Leilei Zhu Chaoguang Tian
author_facet	Wenzhu Guo Jianhua Yang Tianchen Huang Dandan Liu Qian Liu Jingen Li Wenliang Sun Xingji Wang Leilei Zhu Chaoguang Tian
author_sort	Wenzhu Guo
title	Synergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification
title_short	Synergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification
title_full	Synergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification
title_fullStr	Synergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification
title_full_unstemmed	Synergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification
title_sort	synergistic effects of multiple enzymes from industrial aspergillus niger strain o1 on starch saccharification
publisher	BMC
publishDate	2021
url	https://doaj.org/article/9e0203fd9f1a4cd1ba2daf92706f0724
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Synergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification

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