Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants

Abstract The quali-quantitative characterization of the oral microbiota is crucial for an exhaustive knowledge of the oral ecology and the modifications of the microbial composition that occur during periodontal pathologies. In this study, we designed and validated a new phylogenetic DNA-microarray...

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Autores principales:	Carola Parolin, Barbara Giordani, Rogers Alberto Ñahui Palomino, Elena Biagi, Marco Severgnini, Clarissa Consolandi, Giada Caredda, Stefano Storelli, Laura Strohmenger, Beatrice Vitali
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e04b4f33c0941d0b92e1c48d6f5f2ef
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spelling	oai:doaj.org-article:9e04b4f33c0941d0b92e1c48d6f5f2ef2021-12-02T15:05:28ZDesign and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants10.1038/s41598-017-06743-62045-2322https://doaj.org/article/9e04b4f33c0941d0b92e1c48d6f5f2ef2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06743-6https://doaj.org/toc/2045-2322Abstract The quali-quantitative characterization of the oral microbiota is crucial for an exhaustive knowledge of the oral ecology and the modifications of the microbial composition that occur during periodontal pathologies. In this study, we designed and validated a new phylogenetic DNA-microarray (OralArray) to quickly and reliably characterize the most representative bacterial groups that colonize the oral cavity. The OralArray is based on the Ligation Detection Reaction technology associated to Universal Arrays (LDR-UA), and includes 22 probe sets targeted to bacteria belonging to the phyla Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, Fusobacteria, and Spirochaete. The tool is characterized by high specificity, sensitivity and reproducibility. The OralArray was successfully tested and validated on different oral samples (saliva, lingual plaque, supragingival plaque, and healing cap) collected from 10 healthy subjects. For each specimen, a microbial signature was obtained, and our results established the presence of an oral microbial profile specific for each subject. Moreover, the tool was applied to evaluate the efficacy of a disinfectant treatment on the healing caps before their usage. The OralArray is, thus, suitable to study the microbiota associated with various oral sites and to monitor changes arising from therapeutic treatments.Carola ParolinBarbara GiordaniRogers Alberto Ñahui PalominoElena BiagiMarco SevergniniClarissa ConsolandiGiada CareddaStefano StorelliLaura StrohmengerBeatrice VitaliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carola Parolin Barbara Giordani Rogers Alberto Ñahui Palomino Elena Biagi Marco Severgnini Clarissa Consolandi Giada Caredda Stefano Storelli Laura Strohmenger Beatrice Vitali Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants
description	Abstract The quali-quantitative characterization of the oral microbiota is crucial for an exhaustive knowledge of the oral ecology and the modifications of the microbial composition that occur during periodontal pathologies. In this study, we designed and validated a new phylogenetic DNA-microarray (OralArray) to quickly and reliably characterize the most representative bacterial groups that colonize the oral cavity. The OralArray is based on the Ligation Detection Reaction technology associated to Universal Arrays (LDR-UA), and includes 22 probe sets targeted to bacteria belonging to the phyla Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, Fusobacteria, and Spirochaete. The tool is characterized by high specificity, sensitivity and reproducibility. The OralArray was successfully tested and validated on different oral samples (saliva, lingual plaque, supragingival plaque, and healing cap) collected from 10 healthy subjects. For each specimen, a microbial signature was obtained, and our results established the presence of an oral microbial profile specific for each subject. Moreover, the tool was applied to evaluate the efficacy of a disinfectant treatment on the healing caps before their usage. The OralArray is, thus, suitable to study the microbiota associated with various oral sites and to monitor changes arising from therapeutic treatments.
format	article
author	Carola Parolin Barbara Giordani Rogers Alberto Ñahui Palomino Elena Biagi Marco Severgnini Clarissa Consolandi Giada Caredda Stefano Storelli Laura Strohmenger Beatrice Vitali
author_facet	Carola Parolin Barbara Giordani Rogers Alberto Ñahui Palomino Elena Biagi Marco Severgnini Clarissa Consolandi Giada Caredda Stefano Storelli Laura Strohmenger Beatrice Vitali
author_sort	Carola Parolin
title	Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants
title_short	Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants
title_full	Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants
title_fullStr	Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants
title_full_unstemmed	Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants
title_sort	design and validation of a dna-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9e04b4f33c0941d0b92e1c48d6f5f2ef
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Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants

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