Simultaneous readout of multiple FRET pairs using photochromism

Performing multiple FRET measurements at once can be challenging. Here the authors report a method to discriminate between overlapping FRET pairs, even if the fluorophores display almost identical absorption and emission spectra, based on the photochromism of the donor fluorophores.

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Autores principales:	Thijs Roebroek, Wim Vandenberg, François Sipieter, Siewert Hugelier, Christophe Stove, Jin Zhang, Peter Dedecker
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/9e086db852c1424faa90ec134becce7a
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id	oai:doaj.org-article:9e086db852c1424faa90ec134becce7a
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spelling	oai:doaj.org-article:9e086db852c1424faa90ec134becce7a2021-12-02T14:25:25ZSimultaneous readout of multiple FRET pairs using photochromism10.1038/s41467-021-22043-02041-1723https://doaj.org/article/9e086db852c1424faa90ec134becce7a2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22043-0https://doaj.org/toc/2041-1723Performing multiple FRET measurements at once can be challenging. Here the authors report a method to discriminate between overlapping FRET pairs, even if the fluorophores display almost identical absorption and emission spectra, based on the photochromism of the donor fluorophores.Thijs RoebroekWim VandenbergFrançois SipieterSiewert HugelierChristophe StoveJin ZhangPeter DedeckerNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Thijs Roebroek Wim Vandenberg François Sipieter Siewert Hugelier Christophe Stove Jin Zhang Peter Dedecker Simultaneous readout of multiple FRET pairs using photochromism
description	Performing multiple FRET measurements at once can be challenging. Here the authors report a method to discriminate between overlapping FRET pairs, even if the fluorophores display almost identical absorption and emission spectra, based on the photochromism of the donor fluorophores.
format	article
author	Thijs Roebroek Wim Vandenberg François Sipieter Siewert Hugelier Christophe Stove Jin Zhang Peter Dedecker
author_facet	Thijs Roebroek Wim Vandenberg François Sipieter Siewert Hugelier Christophe Stove Jin Zhang Peter Dedecker
author_sort	Thijs Roebroek
title	Simultaneous readout of multiple FRET pairs using photochromism
title_short	Simultaneous readout of multiple FRET pairs using photochromism
title_full	Simultaneous readout of multiple FRET pairs using photochromism
title_fullStr	Simultaneous readout of multiple FRET pairs using photochromism
title_full_unstemmed	Simultaneous readout of multiple FRET pairs using photochromism
title_sort	simultaneous readout of multiple fret pairs using photochromism
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9e086db852c1424faa90ec134becce7a
work_keys_str_mv	AT thijsroebroek simultaneousreadoutofmultiplefretpairsusingphotochromism AT wimvandenberg simultaneousreadoutofmultiplefretpairsusingphotochromism AT francoissipieter simultaneousreadoutofmultiplefretpairsusingphotochromism AT siewerthugelier simultaneousreadoutofmultiplefretpairsusingphotochromism AT christophestove simultaneousreadoutofmultiplefretpairsusingphotochromism AT jinzhang simultaneousreadoutofmultiplefretpairsusingphotochromism AT peterdedecker simultaneousreadoutofmultiplefretpairsusingphotochromism
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Simultaneous readout of multiple FRET pairs using photochromism

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