Simultaneous readout of multiple FRET pairs using photochromism

Performing multiple FRET measurements at once can be challenging. Here the authors report a method to discriminate between overlapping FRET pairs, even if the fluorophores display almost identical absorption and emission spectra, based on the photochromism of the donor fluorophores.

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Autores principales: Thijs Roebroek, Wim Vandenberg, François Sipieter, Siewert Hugelier, Christophe Stove, Jin Zhang, Peter Dedecker
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/9e086db852c1424faa90ec134becce7a
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