Gold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells

Carlos Lara-Cruz,1 Javier E Jiménez-Salazar,2 Marcela Arteaga,2 Michelle Arredondo,1 Eva Ramón-Gallegos,3 Nikola Batina,1 Pablo Damián-Matsumura21Nanotechnology and Molecular Engineering Laboratory, Department of Chemistry, Division of Basic Science and Engineering (...

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Autores principales:	Lara-Cruz C, Jiménez-Salazar JE, Arteaga M, Arredondo M, Ramón-Gallegos E, Batina N, Damián-Matsumura P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Nanotherapy hormone therapy estrogen-induced vesicle formation AuNP cellular uptake membrane roughness endocytosis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9e0ec409463044d4919a729e87456bab
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spelling	oai:doaj.org-article:9e0ec409463044d4919a729e87456bab2021-12-02T03:15:59ZGold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells1178-2013https://doaj.org/article/9e0ec409463044d4919a729e87456bab2019-05-01T00:00:00Zhttps://www.dovepress.com/gold-nanoparticle-uptake-is-enhanced-by-estradiol-in-mcf-7-breast-canc-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Carlos Lara-Cruz,1 Javier E Jiménez-Salazar,2 Marcela Arteaga,2 Michelle Arredondo,1 Eva Ramón-Gallegos,3 Nikola Batina,1 Pablo Damián-Matsumura21Nanotechnology and Molecular Engineering Laboratory, Department of Chemistry, Division of Basic Science and Engineering (DCBI), Universidad Autónoma Metropolitana (UAM), Mexico City, Mexico; 2Department of Biology of Reproduction, Division of Biological Sciences and Health (DCBS), Universidad Autónoma Metropolitana (UAM), Mexico City, Mexico; 3Department of Morphology, National School of Biological Sciences, Instituto Politécnico Nacional, Mexico City, MexicoPurpose: In the present study, we investigated the effects of 17β-estradiol (E2) on membrane roughness and gold nanoparticle (AuNP) uptake in MCF-7 breast cancer cells.Methods: Estrogen receptor (ER)-positive breast cancer cells (MCF-7) were exposed to bare 20 nm AuNPs in the presence and absence of 1×10−9 M E2 for different time intervals for up to 24 hrs. The effects of AuNP incorporation and E2 incubation on the MCF-7 cell surface roughness were measured using atomic force microscopy (AFM). Endocytic vesicle formation was studied using confocal laser scanning microscopy (CLSM). Finally, the results were confirmed by hyperspectral optical microscopy.Results: High-resolution AFM images of the surfaces of MCF-7 membranes (up to 250 nm2) were obtained. The incubation of cells for 12 hrs with AuNP and E2 increased the cell membrane roughness by 95% and 30% compared with the groups treated with vehicle (ethanol) or AuNPs only, respectively. This effect was blocked by an ER antagonist (7α,17β-[9-[(4,4,5,5,5-Pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-triene-3,17-diol [ICI] 182,780). Higher amounts of AuNPs were localized inside MCF-7 cells around the nucleus, even after 6 hrs of E2 incubation, compared with vehicle-treated cells. Endolysosome formation was induced by E2, which may be associated with an increase in AuNP-uptake.Conclusions: E2 enhances AuNP incorporation in MCF-7 cells by modulating of plasma membrane roughness and inducing lysosomal endocytosis. These findings provide new insights into combined nanotherapies and hormone therapies for breast cancer.Keywords: nanotherapy, hormone therapy, estrogen-induced vesicle formation, AuNP cellular uptake, membrane roughness, endocytosisLara-Cruz CJiménez-Salazar JEArteaga MArredondo MRamón-Gallegos EBatina NDamián-Matsumura PDove Medical PressarticleNanotherapyhormone therapyestrogen-induced vesicle formationAuNP cellular uptakemembrane roughnessendocytosisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 2705-2718 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanotherapy hormone therapy estrogen-induced vesicle formation AuNP cellular uptake membrane roughness endocytosis Medicine (General) R5-920
spellingShingle	Nanotherapy hormone therapy estrogen-induced vesicle formation AuNP cellular uptake membrane roughness endocytosis Medicine (General) R5-920 Lara-Cruz C Jiménez-Salazar JE Arteaga M Arredondo M Ramón-Gallegos E Batina N Damián-Matsumura P Gold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells
description	Carlos Lara-Cruz,1 Javier E Jiménez-Salazar,2 Marcela Arteaga,2 Michelle Arredondo,1 Eva Ramón-Gallegos,3 Nikola Batina,1 Pablo Damián-Matsumura21Nanotechnology and Molecular Engineering Laboratory, Department of Chemistry, Division of Basic Science and Engineering (DCBI), Universidad Autónoma Metropolitana (UAM), Mexico City, Mexico; 2Department of Biology of Reproduction, Division of Biological Sciences and Health (DCBS), Universidad Autónoma Metropolitana (UAM), Mexico City, Mexico; 3Department of Morphology, National School of Biological Sciences, Instituto Politécnico Nacional, Mexico City, MexicoPurpose: In the present study, we investigated the effects of 17β-estradiol (E2) on membrane roughness and gold nanoparticle (AuNP) uptake in MCF-7 breast cancer cells.Methods: Estrogen receptor (ER)-positive breast cancer cells (MCF-7) were exposed to bare 20 nm AuNPs in the presence and absence of 1×10−9 M E2 for different time intervals for up to 24 hrs. The effects of AuNP incorporation and E2 incubation on the MCF-7 cell surface roughness were measured using atomic force microscopy (AFM). Endocytic vesicle formation was studied using confocal laser scanning microscopy (CLSM). Finally, the results were confirmed by hyperspectral optical microscopy.Results: High-resolution AFM images of the surfaces of MCF-7 membranes (up to 250 nm2) were obtained. The incubation of cells for 12 hrs with AuNP and E2 increased the cell membrane roughness by 95% and 30% compared with the groups treated with vehicle (ethanol) or AuNPs only, respectively. This effect was blocked by an ER antagonist (7α,17β-[9-[(4,4,5,5,5-Pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-triene-3,17-diol [ICI] 182,780). Higher amounts of AuNPs were localized inside MCF-7 cells around the nucleus, even after 6 hrs of E2 incubation, compared with vehicle-treated cells. Endolysosome formation was induced by E2, which may be associated with an increase in AuNP-uptake.Conclusions: E2 enhances AuNP incorporation in MCF-7 cells by modulating of plasma membrane roughness and inducing lysosomal endocytosis. These findings provide new insights into combined nanotherapies and hormone therapies for breast cancer.Keywords: nanotherapy, hormone therapy, estrogen-induced vesicle formation, AuNP cellular uptake, membrane roughness, endocytosis
format	article
author	Lara-Cruz C Jiménez-Salazar JE Arteaga M Arredondo M Ramón-Gallegos E Batina N Damián-Matsumura P
author_facet	Lara-Cruz C Jiménez-Salazar JE Arteaga M Arredondo M Ramón-Gallegos E Batina N Damián-Matsumura P
author_sort	Lara-Cruz C
title	Gold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells
title_short	Gold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells
title_full	Gold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells
title_fullStr	Gold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells
title_full_unstemmed	Gold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells
title_sort	gold nanoparticle uptake is enhanced by estradiol in mcf-7 breast cancer cells
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/9e0ec409463044d4919a729e87456bab
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Gold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells

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