Acetylsalicylic acid differentially limits the activation and expression of cell death markers in human platelets exposed to Staphylococcus aureus strains

Abstract Beyond their hemostatic functions, platelets alter their inflammatory response according to the bacterial stimulus. Staphylococcus aureus is associated with exacerbated inflammation and thrombocytopenia, which is associated with poor prognosis during sepsis. Acetylsalicylic acid and statins...

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Autores principales:	Adrien Chabert, Pauline Damien, Paul O. Verhoeven, Florence Grattard, Philippe Berthelot, Fabrice Zeni, Laurence Panicot-Dubois, Stéphane Robert, Françoise Dignat-George, Marie-Ange Eyraud, Bruno Pozzetto, Bernard Payrastre, Fabrice Cognasse, Olivier Garraud, Hind Hamzeh-Cognasse
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e0f65c65f85426e9f2d1f6446e28768
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spelling	oai:doaj.org-article:9e0f65c65f85426e9f2d1f6446e287682021-12-02T11:53:08ZAcetylsalicylic acid differentially limits the activation and expression of cell death markers in human platelets exposed to Staphylococcus aureus strains10.1038/s41598-017-06024-22045-2322https://doaj.org/article/9e0f65c65f85426e9f2d1f6446e287682017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06024-2https://doaj.org/toc/2045-2322Abstract Beyond their hemostatic functions, platelets alter their inflammatory response according to the bacterial stimulus. Staphylococcus aureus is associated with exacerbated inflammation and thrombocytopenia, which is associated with poor prognosis during sepsis. Acetylsalicylic acid and statins prevent platelet aggregation and decrease the mortality rate during sepsis. Therefore, we assessed whether these two molecules could reduce in vitro platelet activation and the inflammatory response to S. aureus. Platelets were exposed to clinical strains of S. aureus in the presence or absence of acetylsalicylic acid or fluvastatin. Platelet activation, aggregation, and release of soluble sCD62P, sCD40 Ligand, RANTES and GROα were assessed. Platelet cell death was evaluated by analyzing the mitochondrial membrane potential, phosphatidylserine exposure, platelet microparticle release and caspase-3 activation. All S. aureus strains induced platelet activation but not aggregation and decreased the platelet count, the expression of cell death markers and the release of RANTES and GROα. Acetylsalicylic acid but not fluvastatin limited platelet activation and inflammatory factor release and restored the platelet count by protecting platelets from Staphylococcus-induced expression of cell death markers. This study demonstrates that acetylsalicylic acid limits S. aureus-induced effects on platelets by reducing cell death, revealing new strategies to reduce the platelet contribution to bacteremia-associated inflammation.Adrien ChabertPauline DamienPaul O. VerhoevenFlorence GrattardPhilippe BerthelotFabrice ZeniLaurence Panicot-DuboisStéphane RobertFrançoise Dignat-GeorgeMarie-Ange EyraudBruno PozzettoBernard PayrastreFabrice CognasseOlivier GarraudHind Hamzeh-CognasseNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adrien Chabert Pauline Damien Paul O. Verhoeven Florence Grattard Philippe Berthelot Fabrice Zeni Laurence Panicot-Dubois Stéphane Robert Françoise Dignat-George Marie-Ange Eyraud Bruno Pozzetto Bernard Payrastre Fabrice Cognasse Olivier Garraud Hind Hamzeh-Cognasse Acetylsalicylic acid differentially limits the activation and expression of cell death markers in human platelets exposed to Staphylococcus aureus strains
description	Abstract Beyond their hemostatic functions, platelets alter their inflammatory response according to the bacterial stimulus. Staphylococcus aureus is associated with exacerbated inflammation and thrombocytopenia, which is associated with poor prognosis during sepsis. Acetylsalicylic acid and statins prevent platelet aggregation and decrease the mortality rate during sepsis. Therefore, we assessed whether these two molecules could reduce in vitro platelet activation and the inflammatory response to S. aureus. Platelets were exposed to clinical strains of S. aureus in the presence or absence of acetylsalicylic acid or fluvastatin. Platelet activation, aggregation, and release of soluble sCD62P, sCD40 Ligand, RANTES and GROα were assessed. Platelet cell death was evaluated by analyzing the mitochondrial membrane potential, phosphatidylserine exposure, platelet microparticle release and caspase-3 activation. All S. aureus strains induced platelet activation but not aggregation and decreased the platelet count, the expression of cell death markers and the release of RANTES and GROα. Acetylsalicylic acid but not fluvastatin limited platelet activation and inflammatory factor release and restored the platelet count by protecting platelets from Staphylococcus-induced expression of cell death markers. This study demonstrates that acetylsalicylic acid limits S. aureus-induced effects on platelets by reducing cell death, revealing new strategies to reduce the platelet contribution to bacteremia-associated inflammation.
format	article
author	Adrien Chabert Pauline Damien Paul O. Verhoeven Florence Grattard Philippe Berthelot Fabrice Zeni Laurence Panicot-Dubois Stéphane Robert Françoise Dignat-George Marie-Ange Eyraud Bruno Pozzetto Bernard Payrastre Fabrice Cognasse Olivier Garraud Hind Hamzeh-Cognasse
author_facet	Adrien Chabert Pauline Damien Paul O. Verhoeven Florence Grattard Philippe Berthelot Fabrice Zeni Laurence Panicot-Dubois Stéphane Robert Françoise Dignat-George Marie-Ange Eyraud Bruno Pozzetto Bernard Payrastre Fabrice Cognasse Olivier Garraud Hind Hamzeh-Cognasse
author_sort	Adrien Chabert
title	Acetylsalicylic acid differentially limits the activation and expression of cell death markers in human platelets exposed to Staphylococcus aureus strains
title_short	Acetylsalicylic acid differentially limits the activation and expression of cell death markers in human platelets exposed to Staphylococcus aureus strains
title_full	Acetylsalicylic acid differentially limits the activation and expression of cell death markers in human platelets exposed to Staphylococcus aureus strains
title_fullStr	Acetylsalicylic acid differentially limits the activation and expression of cell death markers in human platelets exposed to Staphylococcus aureus strains
title_full_unstemmed	Acetylsalicylic acid differentially limits the activation and expression of cell death markers in human platelets exposed to Staphylococcus aureus strains
title_sort	acetylsalicylic acid differentially limits the activation and expression of cell death markers in human platelets exposed to staphylococcus aureus strains
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9e0f65c65f85426e9f2d1f6446e28768
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Acetylsalicylic acid differentially limits the activation and expression of cell death markers in human platelets exposed to Staphylococcus aureus strains

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