Molecular detection of Mycobacterium tuberculosis from buccal swabs among adult in Peru

Abstract Tuberculosis (TB) diagnosis relies on a sputum sample, which cannot be easily obtained from all symptomatic patients. Mycobacterium tuberculosis DNA can be detected from oral swabs, a noninvasive, safe alternative sample type; however, reported sensitivities have been variable and likely de...

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Autores principales:	Annelies W. Mesman, Roger I. Calderon, Nira R. Pollock, Martín Soto, Milagros Mendoza, Julia Coit, Zibiao Zhang, Juan Aliaga, Leonid Lecca, Rebecca C. Holmberg, Molly F. Franke
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e0fe21e03234824b968a15e89aeb02f
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spelling	oai:doaj.org-article:9e0fe21e03234824b968a15e89aeb02f2021-12-02T11:57:58ZMolecular detection of Mycobacterium tuberculosis from buccal swabs among adult in Peru10.1038/s41598-020-79297-92045-2322https://doaj.org/article/9e0fe21e03234824b968a15e89aeb02f2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79297-9https://doaj.org/toc/2045-2322Abstract Tuberculosis (TB) diagnosis relies on a sputum sample, which cannot be easily obtained from all symptomatic patients. Mycobacterium tuberculosis DNA can be detected from oral swabs, a noninvasive, safe alternative sample type; however, reported sensitivities have been variable and likely depend on sample collection, processing procedures and host characteristics. We analyzed three buccal swab samples from 123 adults with culture-confirmed TB in Lima, Peru. We compared the sensitivity and specificity of two sample collection devices (OmniSwab and EasiCollect FTA cards) and examined factors associated with detection. DNA was extracted with a commercially available kit and detected via real-time PCR IS6110 amplification. Overall sensitivity for buccal samples was 51% (95% Confidence Interval [CI] 42–60%). Specificity from a single sample among healthy controls was 96.7% (95% CI 83–99.9%). Positive sputum smear and cavitary disease, correlates of disease burden, were associated with detection via buccal swab. Although we observed higher sensitivities with the Omniswab samples, this appeared to be due primarily to differences in patient characteristics (e.g., cavitary disease). Overall, our findings support the potential for a buccal sample-based TB assay. Future work should focus on assay optimization and streamlining the assay workflow.Annelies W. MesmanRoger I. CalderonNira R. PollockMartín SotoMilagros MendozaJulia CoitZibiao ZhangJuan AliagaLeonid LeccaRebecca C. HolmbergMolly F. FrankeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Annelies W. Mesman Roger I. Calderon Nira R. Pollock Martín Soto Milagros Mendoza Julia Coit Zibiao Zhang Juan Aliaga Leonid Lecca Rebecca C. Holmberg Molly F. Franke Molecular detection of Mycobacterium tuberculosis from buccal swabs among adult in Peru
description	Abstract Tuberculosis (TB) diagnosis relies on a sputum sample, which cannot be easily obtained from all symptomatic patients. Mycobacterium tuberculosis DNA can be detected from oral swabs, a noninvasive, safe alternative sample type; however, reported sensitivities have been variable and likely depend on sample collection, processing procedures and host characteristics. We analyzed three buccal swab samples from 123 adults with culture-confirmed TB in Lima, Peru. We compared the sensitivity and specificity of two sample collection devices (OmniSwab and EasiCollect FTA cards) and examined factors associated with detection. DNA was extracted with a commercially available kit and detected via real-time PCR IS6110 amplification. Overall sensitivity for buccal samples was 51% (95% Confidence Interval [CI] 42–60%). Specificity from a single sample among healthy controls was 96.7% (95% CI 83–99.9%). Positive sputum smear and cavitary disease, correlates of disease burden, were associated with detection via buccal swab. Although we observed higher sensitivities with the Omniswab samples, this appeared to be due primarily to differences in patient characteristics (e.g., cavitary disease). Overall, our findings support the potential for a buccal sample-based TB assay. Future work should focus on assay optimization and streamlining the assay workflow.
format	article
author	Annelies W. Mesman Roger I. Calderon Nira R. Pollock Martín Soto Milagros Mendoza Julia Coit Zibiao Zhang Juan Aliaga Leonid Lecca Rebecca C. Holmberg Molly F. Franke
author_facet	Annelies W. Mesman Roger I. Calderon Nira R. Pollock Martín Soto Milagros Mendoza Julia Coit Zibiao Zhang Juan Aliaga Leonid Lecca Rebecca C. Holmberg Molly F. Franke
author_sort	Annelies W. Mesman
title	Molecular detection of Mycobacterium tuberculosis from buccal swabs among adult in Peru
title_short	Molecular detection of Mycobacterium tuberculosis from buccal swabs among adult in Peru
title_full	Molecular detection of Mycobacterium tuberculosis from buccal swabs among adult in Peru
title_fullStr	Molecular detection of Mycobacterium tuberculosis from buccal swabs among adult in Peru
title_full_unstemmed	Molecular detection of Mycobacterium tuberculosis from buccal swabs among adult in Peru
title_sort	molecular detection of mycobacterium tuberculosis from buccal swabs among adult in peru
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/9e0fe21e03234824b968a15e89aeb02f
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Molecular detection of Mycobacterium tuberculosis from buccal swabs among adult in Peru

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